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We narrowed to 798 results for: KIN

Showing: 561 - 570 of 798 results
  1. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time PCR, each...introduced how digital PCR works, let’s explain what kind of assays can be used to sniff out your desired ...
  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative and...
  3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...behind. While commercial kits are available for making small point mutations, large deletions/insertions...-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which the phage...
  4. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ... blue, indicating that your IPTG and x-gal are working as they should be. Don't rush the process: It...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...
  5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22...
  6. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...of the same technical advantages that come with working with prokaryotes. This includes but is not limited...selection rather than antibiotic selection when working with yeast, due to high rates of spontaneously ...
  7. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...about the history. How had investigators done this kind of work before? Musunuru: In the "olden days," not...
Showing: 561 - 570 of 798 results