We narrowed to 904 results for: CAN

...resource for bench scientists everywhere. In it, you can find an introduction to viral vectors and their elements...content in a clean and easy-to-navigate design. You can find it, along with other eBooks in our collection...

...Understanding the different types of CRISPR systems can be as confusing as reading Shakespeare for the first...CRISPR systems are less popular in the lab. This can be attributed to the multi-Cas effector, Cascade....power of Class 1 systems. Class 1 CRISPR systems can be further broken down into type I, type III, and...CRISPR systems. Type III systems are unique, as they can technically target both RNA and DNA, though it is...that they are involved in plasmid competition or can hijack machinery from other CRISPR systems to carry...people are familiar with. Class 2 CRISPR systems can be further broken down into type II, type V, and ...alternatives to Cas9. Cas12 is unique because it can process multiple gRNAs under a single promoter, allowing...

...these multi-component systems can be complicated to implement, they can be highly specific with a small...comfortable with it or can find a colleague to help you, synthesizing your own ligands can open up more possibilities...more). So, labeling a HaloTag or SNAP-tag construct can help you better detect dim structures or low levels...the same imaging workflows you already use. Or you can really capitalize on the dye’s performance and use...’re working with purified proteins in vitro, you can even use the same tag for multiple proteins but label...limited to fluorescent ligands! Self-labeling tags can also be used for surface immobilization, purification...

...transfected it into your target cells, now what? How can you tell if the protein you are so keen to study ...detect the presence or absence of a protein, but can also determine if proteins are being up- or downregulated...standards, detect the cellular location of proteins, and can be a readout for protein interaction studies such...standard of known molecular weight in a separate lane can help confirm the size of the protein of interest....high binding capacity. In addition, PVDF membranes can be repeatedly stripped of an antibody and reprobed...plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your search, first pay...useful readout for a number of cellular assays. They can determine protein interactions following immunoprecipitation...

...yielding 1-10% knock-in. Read on to learn how you can make CRISPR mouse model generation easi-er! Generating...process takes ~8-10 months. With CRISPR (B), you can inject CRISPR components directly into a zygote and...repair, with researchers hypothesizing that they can efficiently integrate into the DNA through an HDR...sequence or place it far from the PAM; if not, you can add a silent PAM or guide mutation. Designing an ... How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template!...insertion feature (e.g., a fluorescent protein), you can amplify the desired region using primers containing...the ssDNA construct as described in Figure 2.  You can also use Addgene plasmids to purify Cas9 protein....

...presentations and interviews. But how can I adapt this setup for the lab, so we can get back to producing protocol...for free. We’re a small operation, so sometimes it can be a big challenge. But ask any filmmaker or videographer...on-screen, somebody who really knows the protocol and can guide viewers through. I know cameras, but we needed...the scenes footage in the last several years, you can usually see a director sitting in their chair not...some more important lab work from being done. You can watch the first video in our Intro to the Lab Bench...

... aptamer? RNA is not inherently fluorescent and can be challenging to track intracellularly, but aptamers...to cells. Because it rapidly photobleaches, DFHBI can only be used for qualitative, but not quantitative...expression of coding RNA. Spinach-based riboswitches can be used as stable metabolite sensors for imaging ...developed in a study of artificial guide RNAs that can be used to simultaneously target cellular RNA to ...Broccoli has been developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer...observed when these RNAs complex and the entire aptamer can hybridize and fold. (Alam et al., 2017). Yellow fluorescent...selection and directed evolution." Journal of the American Chemical Society 136.46 (2014): 16299-16308. PubMed...

...school has its ups and downs for everyone but you can come out on top if you leverage your community, think...'s) thesis defense. This wonderful piece of work can be found here: https://www.youtube.com/watch?v=lQGAsgjjBDI...post-doc project 19:55 - 22:40 : Improvements that can be made to the grad school process 22:41 - END : ...beyond graduate school. Ben J. Vincent is a Ph.D candidate in the Program in Genetics and Genomics at Harvard...

...different experiments/conditions can change binding ability – i.e., the antibody can no longer bind to the target...-target proteins), variability (separate batches can perform differently – happens when the antibody is...antibodies onto the mAb3D atlas website. This website can then be used by thousands of scientists to confidently...antibodies because of their immunoglobin component that can be produced in large “copies.” This homogeneity circumvents...antibodies cause research that cannot be reproduced and projects that cannot be completed. A 2015 review ...

...particularly excited about the dTAG system as we can use it to evaluate the effects of rapid and selective...approaches. In addition, the dTAG system is versatile and can be used in both cell-based assays and mouse models...protein-of-interest, it is important to determine which terminus can accommodate the FKBP12F36V tag without disrupting...disrupting protein function. A literature search can help determine which terminus is favorable for tagging. However...terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality...Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted protein degradation In the ...PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. He is a chemical biologist interested...