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Addgene

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We narrowed to 892 results for: CAN

Showing: 571 - 580 of 892 results

  1. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...disease is caused by a mutation in a single gene, it can potentially be treated by correcting the mutation.... However, many diseases require techniques that can deliver gene therapies directly to the cells that... tools such as nanoparticles. These technologies can have serious adverse effects, and their immunogenic... human population. These pre-existing antibodies can interfere with virus-cell interactions and seriously...the immune system – patients’ own immune systems can develop antibodies that neutralize the AAV vectors...disease, and HIV. A notable landmark study showed significant clinical efficacy against a form of blindness... of LCA and mutations in RPE65 (n=3), showed significant improvements in vision over a period of 1 to ...

  2. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...particularly excited about the dTAG system as we can use it to evaluate the effects of rapid and selective...approaches. In addition, the dTAG system is versatile and can be used in both cell-based assays and mouse models...protein-of-interest, it is important to determine which terminus can accommodate the FKBP12F36V tag without disrupting...disrupting protein function. A literature search can help determine which terminus is favorable for tagging. However...terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality...Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted protein degradation In the ...PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. He is a chemical biologist interested...

  3. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...different experiments/conditions can change binding ability – i.e., the antibody can no longer bind to the target...-target proteins), variability (separate batches can perform differently – happens when the antibody is...antibodies onto the mAb3D atlas website. This website can then be used by thousands of scientists to confidently...antibodies because of their immunoglobin component that can be produced in large “copies.” This homogeneity circumvents...antibodies cause research that cannot be reproduced and projects that cannot be completed. A 2015 review ...

  4. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...microelectronic fibers made from thermoplastic polymers that can work with all the bells and whistles. The fibers ... molecules.   Fun fact: A 1 cm block of polymer can generate many meters of fibers!     Fig...its associated fibers across multiple channels and can also record tissue temperature proximal to the implanted...a rechargeable battery, is relatively small, and can be detached easily whenever necessary. This is all...

  5. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...what appears to be a funky old garage, but looks can be deceiving. Step into this building and you will...volunteers. Transforming citizens into scientists Anyone can become a member of BosLab and previous laboratory...environment, and openly publish protocols so that anyone can engineer yeast to do similar things. The focus is... To learn more about what we’re up to or how you can help BosLab, visit our website and follow us on twitter...

  6. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...this can lead to wasted time or retractions. Using a catalog number for identifying a reagent can be helpful...specific Addgene ID # listed in a publication, you can quickly pull up the associated plasmid page on the...87155 ; RRID:Addgene_87155).” So when citing, you can copy and paste the text attributing the PI who generated...

  7. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...protein are overexpressed in cells, microtubule growth can be switched off with blue light which causes LOV2...recently developed “Riboglow,” a set of tools that can be used to visualize RNA localization in mammalian... tags using the same number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches...APOBEC1. Finally, they show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting its ...This paper demonstrated that the migration of a cancer cell line equipped with photodissociable EB1 could...

  8. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...record yourself. You can watch yourself to critique and figure out what to improve. You can share it with others...charge before others do”. Candidates who have “started something” which they can share on their resumes ...There are many different kinds of grants that you can apply for. If you are in a lab, your PI is likely...to work-from-home and just now realizing that we can’t bide our time and wait for it to be over to work...roles but for almost every position, we look for candidates that have demonstrated initiative. In fact, hear...have a definite advantage in our process. We see candidates who have done the following types of things: ...relationships when you are more desperate or needy so you can’t wait until you imminently need a job. Start now....

  9. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...-inducible Proteins (FLiPs) Fluorescent proteins can be applied in a long list of creative ways. In her...photoswitchable fluorescent proteins to create enzymes that can be turned on and off with light.  Xin hypothesized...presented her work at the 2017 Winter qBio Meeting. You can learn more about Xin’s work with photoswitchable ...expression of GFP from the lac operon. GFP expression can then be used to determine the amount of HMO originally...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...

  10. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...calcium sensors, which are available at Addgene, can be used for ratiometric in vivo imaging. Griesbeck...on" in the presence of calcium. This color change can be observed under a microscope using commercially... beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila, C. elegans, and zebrafish...and his colleagues took to optimize sensitivities can now be applied to dozens of other published FRET-...
Showing: 571 - 580 of 892 results