...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...blog posts Learn more about Addgene Resources on the Addgene website Find plasmids for your research  ...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...and the opportunities we have for the future. At Addgene we will never ever stop planning, perfecting, and...new projects. Diversification is happening. The Addgene team will have some fun challenges this year and...get excited about making their first deposit to Addgene. We are also lauded by another set of stakeholders...we received some entirely unsolicited praise: “Addgene, for one, is a sterling example of collaboration...

...first antibodies distributed by Addgene.  The plasmids used to generate these antibodies were based off... used to generate cDNA, which  was used as a template for PCR amplification with a degenerate primer set...Introduction to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the construction...et. al., Elife  A limitation of hybridoma generated mAbs is that if the frozen cell line becomes non-recoverable...grow up the cells. This RNA could then be used to generate functional recombinant monoclonal antibody (R-...

...Antibody, Addgene gave to me… The Addgene Antibody Data Hub!    🎶On the sixth day of Antibody, Addgene gave...Antibody, Addgene gave to me…  An Antibodies 101 eBook!  🎶On the second day of Antibody, Addgene gave to...Antibody, Addgene gave to me…  An online Antibody Guide!    🎶On the fourth day of Antibody, Addgene gave ...Antibody, Addgene gave to me… Antibodies developed by IPI!   🎶On the eighth day of Antibody, Addgene gave ...Antibody, Addgene gave to me…  A recombinant Anti-APP!   🎶On the eleventh day of Antibody, Addgene gave to...Anti-DYKDDDDK!   🎶On the ninth day of Antibody, Addgene gave to me… A neuroscience antibody collection!...flow plots!   🎶On the twelfth day of Antibody, Addgene gave to me…  A growing recombinant antibody collection...

...new to Addgene this year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s catalog...imaging. Vladislav Verkhusha’s lab has generated a new genetically encoded calcium indicator using small...on the Addgene blog Fluorescent Proteins 101: Fluorescent Biosensors Resources on Addgene.org Fluorescent...Here at Addgene’s headquarters, the skies are getting dark and wintry with the end of year approaching...other targets. Figure 1: MTRIA biosensors are generated by inserting a circularly-permuted GFP into an...localized for synaptic glutamate response. They generated variants optimized for different applications ...St-Pierre lab and colleagues have developed a new genetically-encoded voltage indicator (GEVI) called JEDI-...

...learning.” Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since...work at Addgene. I posed this question to all nine of my interviewees. Fabienne Furt, Addgene’s Associate...resources on the Addgene blog Browse our science career blog posts Download Addgene's science career guide... guide Resources on Addgene.org Learn more about working at Addgene ...Flynn and I am currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member...go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community...community college when she took her first job at Addgene as an Order Processing Assistant. After a few months...

...Resources on the Addgene Blog Learn About the Addgene Help Center Get Help with the Addgene Deposit Process...emails in my six years as a senior scientist at Addgene. Having spent that long in customer service, I'...Though my experience is based solely on my time at Addgene, I’m confident that these tips and tricks will ...another great source of information. At least at Addgene, the questions in our FAQ section are really, truly...not realize it, all of the reagents available at Addgene come from researchers, and the vast majority of...that for you” meme is infamous enough to have generated it’s own tongue-in-cheek website.  If you are ...happens more frequently than you might think. The Addgene scientists answering the phone are smart, but they...

...from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation Toolbox from Addgene     ...only antibodies. (A) Overview of genetic modifications made to generate the nanomouse. (VHH = variable ...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...to human populations around the world. Here at Addgene, we love camelids too, though not just for the ...chromobodies,” which are single-domain antibodies genetically fused to fluorescent proteins and used to visualize... synthetic single-domain antibody libraries to generate new sdAbs. There has also been some interesting...) (Xu, et al., 2021).      Figure 2: Genetically modified mice offer an alternative method to ...

...been generated for yeast (Kim et al., 2019) and fruit flies (Bischof et al., 2018), the generation of such...quantities (and the correct stoichiometric ratios) to generate useable data. What’s more, measuring and quantifying...straightforward nature of BiFC also makes it easier to generate a large number of constructs for high-throughput...screening of PPIs. For example, Bischof et al. (2018) generated an open reading frame (ORF) library to screen ... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for...pair. Find BiFC plasmids for your experiment at Addgene! How to Design a BiFC Experiment Much like FRET...optimized to be successful. A BiFC experiment generally involves three steps: fusion protein construction...

...scientists are generous Besides some of the scientists I've already mentioned, Addgene depositors Marius...What an honor for Addgene to be a part of that!  Additional Resources on the Addgene Blog Learn about...landmark Cell paper showing that it was possible to generate induced pluripotent stem cells (iPSCs) capable...called OSKM factors). As the Yamanaka lab's primary Addgene contact for many years, I've been looking forward...example of the fast movement in this field.  As an Addgene scientist, I'm exposed to many scientific communities...clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work with Parkinson's...quality control process alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took...

...communication blog posts Resources on Addgene.org Find careers at Addgene ... project I’d seen in my already long tenure at Addgene. I would work with our research team and software...pinpointed and (mostly) solved two main problems with Addgene’s cross team communication. Although these real ...these plates now” to a fellow team member. But, at Addgene, we use seven different types of “plates” (Fig....not the container was barcoded (this is pretty Addgene specific) The types or purposes of each given container...that there were a lot of words that nearly all Addgene teams used that seemed simple, but they could in...solution for this problem was the creation of an Addgene lexicon. The base lexicon was and continues to ...