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Addgene

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We narrowed to 892 results for: CAN

Showing: 581 - 590 of 892 results

  1. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...recommends to seek out advice from seasoned PIs who can offer support. “It is an exciting and emotional time... not rush when growing your lab. “One bad choice can be counter-productive." He also emphasizes the point... at about 65,000 messages and dealing with email can be half of my day sometimes.” He points out that ... to companies.” Take home message Starting a lab can be a challenging process. Each PI emphasizes the ... team so getting everyone talking to each other cannot be undervalued.” Connie Cepko points out the limited...

  2. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    Published
    Feb. 25, 2021, 2:15 p.m.
    ...a student or postdoc ends up in a lab where they can learn the plus skills needed to succeed on the career...disseminating career training approaches in a way that can be implemented widely in the community.  Talk alone...scheduled for March 4th from 2:30-4:00 pm ET.  You can learn more about the report's recommendations and...

  3. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...process of starting a forum where non-scientists can directly approach scientists and doctors with their...visual representation of science to our audience. How can researchers work with you? We welcome researchers...contributing to The Addictive Brain. A contribution can either be an article, a short post, a video, or a...

  4. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ... we have to change to ensure that all scientists can have successful careers doing science in some form...the upcoming "Future of Research" conference. You can register for the conference here. The Future of Research...programs or peer mentoring groups. But for now, you can click here to register and join your postdoc colleagues...

  5. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...DNA sequencing troubleshooting and analysis 1. You can use any of the following programs to view your .ab1...all inclusive service some companies offer), you can purify the product either via the Sodium Acetate/...strongly recommend using a silica spin column. They can be pricey, but well worth it. 6. Edit your DNA ...

  6. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...tools.  Distributing and depositing In Figure 1, you can see cumulative numbers of CRISPR plasmid distribution...categorized by function, from 2014 to 2023. As you can see, nucleases, the original CRISPR tool, remain ...the most popular with our requestors. However, we can again see quick adaptation and steady use of all ...categorized by “age” or year they were deposited, you can see that from 2013 and on, popular CRISPR plasmids...ordering the plasmids is the easy part; using them can be a bit more challenging. Addgene’s free educational...other countries, from Japan to Australia, are significant depositors and requestors. CRISPR tools are developed...

  7. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...cell genome can be packaged inside the vectors during production. These impurities cannot be removed during...capabilities and Seqwell’s plexWell technology, we can now perform NGS at Addgene to sequence all the DNA.... In most cases, the source of the contamination can be identified, and we will discard any AAV prep we...developed custom open-access Python scripts that can perform a deeper analysis of the data. Our serotype...Next-generation sequencing (NGS) is a powerful tool that can be used to identify DNA contaminants in AAV preparations...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...were first designed in 2005. The DNA-binding domain can be engineered to target specific DNA sequences while...then induces a double strand break. These breaks can then be repaired by homologous recombination with...triplet and when several ZFNs are strung together, it can read many base pairs simultaneously to increase specificity...geneticist named Zobrist who designs a virus that can potentially kill one-third of the human beings (just...rely on the cellular DNA repair machinery, which can lead to high frequency of indel formation in the ...called pegRNA (prime editing RNA), the prime editor can install all possible point mutations, delete short... understanding of the adenine base editors which can help accelerate the engineering of novel base editor...

  9. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...critical control in biological experiments. Replicates can be technical or biological and are included to improve...such as bovine serum albumin. Secondary antibodies can also react with endogenous antibodies naturally present...in mouse tissue. Such species-on-species staining can be partially addressed with stringent staining protocols...way to reduce, if not eliminate, the noise. This can be done either by modifying the blocking conditions...autofluorescence, also interferes with data interpretation and can be addressed with a label control. In a label control...allows you to detect dead or dying cells so that you can base your analysis on living cells only (for example...results, the signal from your target needs to be significantly higher than the background noise (Waters 2009...

  10. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... to specific subcellular regions, laser ablation can damage other cellular structures, and genetic mutations...Restricting the effect to a specific time or location can be critical when, say, the gene is essential during...with dozens of family members and many functions it can be difficult to unravel their specific roles. Zebrafish...using directed evolution (Tei et al., 2023).  PLD can hydrolyze phosphatidylcholine (PC), the predominant... membrane lipid, to phosphatidic acid (PA) or it can swap phosphatidylchonine’s choline head group for...to plasma membrane or lysosomes and its activity can be monitored by phosphatidic acid probe GFP-PASS....
Showing: 581 - 590 of 892 results