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We narrowed to 803 results for: Cre

Showing: 581 - 590 of 803 results
  1. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...
  2. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...solution is more complicated. You’ll need to be creative to be able to work with others to come up with...
  3. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...must be utilized or a new combination needs to be created in advance of the experiment. The marker provided...physiological levels due to high copy numbers. This creates a potential metabolic burden on the yeast cells...
  4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...the group mentoring format Adding peer advice increases the diversity of input and perspectives Provides...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...
  5. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com     The modularity of antibodies...antibody and common antigen binding fragments. Created with biorender.com.   Why Fabs?  Antibody fragments...
  6. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...including rapidly depleting a protein-of-interest, increasing target selectivity, overcoming resistance to ...molecule binders. To overcome this limitation, we created a generalizable tag-based strategy, which we refer...
  7. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...excitation light less efficiently. Similarly, decreasing the power of the excitation light reduces the... excitation/emission cycles per unit of time, decreasing the likelihood that a fluorophore will bleach...
  8. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...In both cases, red fluorescence in the vacuole increased with longer exposure to autophagy-inducing conditions...
Showing: 581 - 590 of 803 results