...approaches to control transgene expression by restricting expression to genetically defined cell types. ...neuroscientists have begun to use more precise genetic tools: activators, inhibitors, sensors, recombinases...vectors (AAV), neuroscientists can combine classical genetic tools with the manipulation of specific neuronal...changes affect the phenotype. Viral vectors and genetic approaches are continuously refined and developed...molecular tools and sensors Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT, with AAV.... Specific transgene expression can be also achieved in nontransgenic animals by using cis regulatory ... one location can result in expression of an optogenetic payload in neurons that project to different ...

...CRISPR, Addgene gave to me: a primer on prime editing.  On the eleventh day of CRISPR, Addgene gave to...all of them here.  On the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian... mammalian use. On the second day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing...editing efficiency. On the third day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation ... Delivery (SEND). On the fourth day of CRISPR, Addgene gave to me: enhanced prime editing systems through...manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me: efficient CRISPR editing with a hypercompact...engineered guide RNAs. On the sixth day of CRISPR, Addgene gave to me: PRIME-del (Precise genomic deletions...

...analyze and annotate any DNA sequence. SnapGene Viewer: The free SnapGene Viewer is great for looking at plasmid... biology tools.  Addgene Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors...Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA.   DNA / .../ protein database search and analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs...restriction enzyme cut sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled...available sources, as well as those deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences...tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence manipulation...

...C-terminal region of the human proto-oncogene c-Myc. Since the generation of the first anti-c-Myc [9E10] (...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...approach and outcomes of the screen for improved genetically-encoded voltage indicators (GEVIs) based on the...kinetics. They demonstrate the use of HcKCR1 as an optogenetic silencer in experiments with mouse cortical neurons... HcKCR2, making it more suitable for in vivo optogenetic use.   Fig. 2: A) HcKCR1 is highly selective...control K+ gradients and can be used as a potent optogenetic silencer. The current work also proposes promising... light-gated potassium channels that mediate optogenetic inhibition. Nat Neurosci 25, 967–974 (2022). ...

...preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the... AAV1 Optogenetics Karl Deisseroth New serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...other new AAV vectors as well — including next-generation biosensors for GABA, dopamine, serotonin, and...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV5 Optogenetics Ofer Yizhar New viral prep pAAV_hSyn-DIO-PdCO-mScarlet-WPRE...-PdCO-mScarlet-WPRE AAV1, AAV5 Optogenetics Ofer Yizhar New viral prep with multiple serotypes pAAV-Syn-FLEX-rc...

...pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also expanded our inventory of chemogenetic AAV for activating...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional...FDA-approved drugs. See our inventory of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name ...AAV5 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) New chemogenetics AAV Plasmid Serotype Name 119741  AAV5 ...pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors New optogenetics inhibitors Plasmid Serotype Name 99039 ...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ...

... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!)...Streamlined Processing: When you place an order, Addgene generates the MTA and emails it straight to you, or ... on collaboration (it’s the heart and soul of Addgene’s mission), but legal complexities can arise when...institutions require them for material transfers. When Addgene entered the plasmid sharing space, we heard over...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological... review by the Provider Limited liability Addgene makes it easy Even with all those benefits, we ... handle all the paperwork. Record Keeping: Addgene will let you know exactly how many times your materials...

...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...candidate regulatory kinase is known, it’s possible to generate non-phosphorylated recombinant protein and perform...Creating phosphoserine-incorporated proteins The generation of pSer-incorporated proteins requires a molecular...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized...position. Practical tips for your experiments To generate as much pSer-incorporated protein of interest ...

... biology over the past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers...proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp Addgene depositor...2020! Additional resources on the Addgene Blog Read other Addgene news Find the most recent plasmid ...posts Resources at Addgene Browse the repository Find educational resources at Addgene Search for plasmids...discovery. We’ve documented many new tools on the Addgene blog and have created and updated guides on various...blogger Joachim Goedhart shares some strategies for generating GFP fusion proteins. From considering protein... by leaving a comment or emailing us at blog@addgene.org.  Thank you again for a fantastic year and happy...

...executive director of Addgene, it is my privilege to uphold the values that shape Addgene’s mission and work...thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed 188,729 materials to 39,844 scientists... three–year project that touched every team at Addgene. As a resource, it will allow scientists around...Institute for Protein Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed...focus on expanding international access to the Addgene repository, partially through work with our distributors...mention that 2024 is an incredibly special year for Addgene: it marks our 20th anniversary. Throughout the ...months, we’ll also share our work in evolving Addgene’s strategic vision for the future. Over the years...