... team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed itself ...compatible with optogenetic control. The researchers also showed that Archon1 is functional in a wide range of...activity using the fluorescent voltage indicator Archon Article contributed by Shreya Vedantam  Listen ...membrane where cell voltage can then be measured. Archon1 is  bright, exhibits good localization, has high...models such as mice, C.elegans, and Zebrafish. Find Archon1 plasmids at Addgene! Piatkevich KD et al, Nat Chem...

...assign four different barcodes to each of the gRNAs and to embed these barcodes within the gRNA sequences...method that makes use of guide RNAs with internal barcodes (iBARs) in their loop region. After infecting ...Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just a fraction...titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively...labeled), intermediate (12%), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell...interest in the cells you are studying. Read our SPARC blog post for more details. Figure 2: Dense...

... electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...into a beta-galactosidase cassette allowing a researcher to perform blue-white screening after transformation...

...associated with neuronal activity, such as Fos, Egr3, and Arc. Thus, CRISPRa represents a powerful method to dissect...commentary on their application to neuroscience research. What is CRISPR-based activation? To summarize...immunohistochemistry, it is not amenable to FACS.  Thus, the researchers inserted human CD8, a gene that encodes a surface...

...based on research that has been published, but many discuss in-progress research, proposed research looking... like ResearchGate, GoogleScholar, Academia, ORCID, and MyScienceWork all help us share research. Open... Article, since the 1980s researchers have been increasingly citing research from outside of their disciplines...support researchers as they try to keep up with the growing amount of interdisciplinary research while ...their own work. Of course not all research has to be influenced by research in other disciplines. But there...video     Video abstracts about research, especially published research, have grown in popularity over ...publishers and researchers have Facebook pages, few share any details about their research itself. Pinterest...

... data sources used in research; (2) connectivity to other tools used in research; and (3) connectivity...inter-operability with other research tools and infrastructure in use at research universities not only today...the record of research compiled in the ELN. Without such references the record of research is incomplete... Connectivity to other tools used in research Just as researchers need to access and reference external...used in producing research data, and without access to them, the record of research captured in the ELN...about research that can take place in Slack normally never become part of the permanent research record...for making research data available for public access, review, and query. Gates Open Research and Wellcome...

...shift towards prioritizing research into this disease. A key reason this research has been able to progress...COVID-19 research? We decided to give it a try, by creating a service for COVID-19 researchers. So far,...translational impact of research Universities have also been stepping up to support COVID-19 research. The new Center...to any researcher studying COVID-19. “Open scientific sharing is important for creating research synergies...impact of research activities. By streamlining the development and accessibility of research resources...on methods for coronavirus research, sharing resources for COVID-19 research in their field including datasets...other research, scientific sharing will remain an extremely important part of the future of research. To...

...of biological research that are often forgotten or ignored. If we wish to conduct research that fails less...gender analysis into research. Why are sex and gender analysis important? Doing research wrong costs lives...experiments—which can lead to failed research. For example, an international research team from Norway and Australia...variable in all NIH-funded research. Janine Clayton, Director of the Office of Research on Women’s Health, emphasizes...common worry for researchers wanting to consider sex is cost. Especially in animal research, they worry about...of doing the research correctly from the get go. The future of sex and gender in research There is much...innovation and discovery. Tackling sex and gender in research Governments, universities, and, increasingly, ...

...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach...Organize your research vision into the chalk talk format Once you have developed your research vision, you...Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute....hour. You will get 5-10 minutes to present your research vision uninterrupted and then you will begin working... steps to chalk talk preparation: Define your research vision. Organize your vision into a chalk talk...talk format. Practice. Define your research vision A chalk talk could be your plans for your first NIH ... A well-defined outcome makes it easier for the search committee to understand your goal, what you expect...

...these solutions to your own research and thereby help move the biological research enterprise towards greater... reliable reagents for future research. I’ve talked to many researchers who have complaints about the ...working hard to enable researchers by making it easier to find information about research tools. BenchSci collates... Reproducibility Overview - Jeffrey S. Flier, Researcher at Harvard Medical School, former dean of the... every study should be reproducible. Indeed, researchers are pushing the limits of knowledge as they develop...irreproducibility undermines public trust in science and research. Nonetheless, I’d like to steer clear of saying...Inability to repeat previously performed experiments Researchers should be able to follow one another’s protocols...