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Showing: 51 - 60 of 630 results

  1. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...Johnny Kung, Director of New Initiatives for the Personal Genetics Education Project (pgEd). Advances in...exacerbating existing inequalities and injustices. The Personal Genetics Education Project (pgEd), where I currently...conversations about both the benefits as well as the personal, social and ethical implications of genetics. ...cultural or religious affiliation, and ethnic or personal identity. We do this through a variety of activities...activities, including creating educational materials and lesson plans that are made freely available online; running...translating all these into social media and blog posts, lesson plans, or information briefs for policymakers. ...Kung is the Director of New Initiatives for the Personal Genetics Education Project (pgEd). He is broadly...

  2. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...labelled with horseradish peroxidase (HRP; Kristensson and Olsson, 1971), plant lectins (wheat germ agglutinin...24336151. PubMed Central PMCID: PMC4004172. Kristensson, K., and Olsson, Y. (1971). Retrograde axonal transport...difficult to accurately map network connections (Lo & Anderson, 2011). In the past two decades, several virus...PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R., Holzbaur, E.L., and Wolfe...29, 363–365. PubMed PMID: 4107258. Lo, L., and Anderson, D.J. (2011). A Cre-dependent, anterograde transsynaptic..., J.S., Hyman, B.T., and McLean, P.J. (2009). Comparison of transduction efficiency of recombinant AAV... V., Thiry, I., Deroose, C. M., Mortelmans, L., Wilson, J.M., Debyser, Z., and Baekelandt, V. (2007). ...

  3. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...a price of a few dollars per reaction. By comparison, Gibson assembly can cost over $15.00 per reaction...In today’s post, we will focus on a high-level comparison of popular cloning methodologies, with a special...as 30 minutes! Alternatively, you might consider Gibson cloning. In this technique, you first perform a...reaction if you purchase a pre-made mix. Nonetheless, Gibson assembly is a highly versatile and efficient way...propagating. The ligation-independent methods like Gibson and SLIC are also quite efficient; although efficiency...them are some of the homology based methods, like Gibson or SLIC, since one can pool the components and ...

  4. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...students who are unable to attend in-person interviews for a variety of reasons (logistical, financial, etc…)...visit in person, things like sickness or scheduling issues can and do interfere with those in-person plans...COVID-19 and standards for hybrid, virtual, and in-person events change seemingly daily (and are inconsistent...about the program and having the time to ask personalized questions might be difficult. However, an online...Even if you had planned a campus visit and the in-person portion got canceled, taking advantage of virtual...

  5. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...used to call copy numbers should be as small as reasonably possible and set using the variance seen within...negative for each reaction, math happens (this time, Poisson statistics), and the readout gives an absolute ... through concentration values, rather than by comparison to a standard curve, and most results can be ...7778-9_9. PMID: 29717442. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel HR. Basic principles... S., Hollox, E. J., & Boon Peng, H. (2015). A comparison of assays for accurate copy number measurement...journal.pone.0116791 Providenti MA, O'Brien JM, Ewing RJ, Paterson ES, Smith ML. The copy-number of plasmids and ... Rachel Leeson...

  6. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ... of a two-part interview with Vini Mani and Amy Gilson from Science in the News at Harvard University....your science, we sat down with Vini Mani and Amy Gilson from Science in the News, a graduate student organization...Podcast Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani is a graduate student at Harvard University...News, and member of the SITN podcast team.     Amy Gilson recently received her PhD from Harvard University...SITN podcast team. You can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer...McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use Reddit for Science Communication ...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...changes in specificity than AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX...happy transducing! This post was written by Rachel Leeson, with significant contributions from Cynthia Arokiaraj...Altermatt, M., Dobreva, T., Chen, S., Wang, A., Thomson, M., & Gradinaru, V. (2021). Deep Parallel Characterization...s41565-023-01419-x Deverman, B. E., Pravdo, P. L., Simpson, B. P., Kumar, S. R., Chan, K. Y., Banerjee, A....., Wang, Q., Katz, N., Buza, E. L., Bell, P., & Wilson, J. M. (2018). The Neurotropic Properties of AAV-PHP.B.... J., Bell, P., Raymond, A., Stanford, W. L., & Wilson, J. M. (2019). The GPI-Linked Protein LY6A Drives...journal.pbio.3002112 Jang, M. J., Coughlin, G. M., Jackson, C. R., Chen, X., Chuapoco, M. R., Vendemiatti,...

  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...them for use in your lab. Structural Comparison Functional Comparison Advantages of Nanobodies How to Use...for a comparison of nanobody, HCab, and traditional IgG antibody structures. Functional comparison Strong...Nanobody Toolbox Find Nanobody Plasmids Structural comparison As the name suggests, nanobodies are smaller ...Large: Advantages of Antibodies One of the main reasons antibodies are great indirect immunodetection tools... antibody incubation. See figure 4A below for comparison of this one-step staining with nanobodies vs ...

  9. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... is adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify...DNA gel extraction method described in Kim and Morrison is nearly identical to that of commercially available...Purify DNA using glass filters. Image: Kim and Morrison, 2009. This method provides DNA yield and quality... kits for different purposes, with the Kim and Morrison method, you can just adjust the number of glass... protocol video library References Kim, Y., & Morrison, S.L. (2009). A Rapid and Economic In-House DNA...://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G., Wang, Y., Wee, E.J., Turni, C., Blackall,...

  10. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...out their own novel experiments in semester two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates...capabilities and laboratory and critical thinking skills (Knutson et al., 2010a,b). Classroom-based research projects...before a CURE trainee goes on to win a Nobel!   Jason Pellettieri is Professor and Chair of the Biology...Einstein Discovers America. New York./  Callahan, K., Peterson, C., et al. 2022. External collaboration results...planarians. Developmental Biology. 457:119-127. Knutson, K., Smith, J., Wallert, M., Provost, J. 2010a....and Molecular Biology Education. 38(5):317-323. Knutson, K., Smith, J., Nichols, P., Wallert, M., Provost...Education. 38(5):324-329. Kowalski, J., Hoops, G., Johnson, J. 2016. Implementation of a collaborative series...
Showing: 51 - 60 of 630 results