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We narrowed to 331 results for: nans

Showing: 51 - 60 of 331 results
  1. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...techniques. For example, restriction mapping led to recombinant DNA technologies that are now commonplace in ...sequencing world, one up and coming innovation is nanopore-based DNA sequencing which reads the nucleotides...nucleotides (8). Compared to older technologies nanopore-based DNA sequencing machines are relatively small...have high error rates (10). It is projected that nanopore-based sequencing technologies could one day sequence...PubMed PMID: 1925562. 10. Feng, Yanxiao, et al. "Nanopore-based fourth-generation DNA sequencing technology...
  2. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...focus on defining these resources and not on the financial aspects. Money often carries an emotional weight...administrative services, managers, and techs’ financial books.  To find personnel salary ranges, go to...specific aims. 2. Then make decisions based on financial values. All too often, we see early investigators...save you money in the short term. However, the financial risks associated with old equipment, like service.... Always focus on the science When negotiating finances, instead of solely discussing numerical values...
  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...brain samples from the James Trimmer Lab, using recombinant antibodies with different isotypes. (A) Neocortex... affinity reagents like fluorescently-labeled nanobodies or scFvs. Many, many options for fluorescently.... (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...Beuschel, R. T., Kabat, J., Croteau, J., Davis, J., Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for...
  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...fluorescently tagged nanocellulose for nanotoxicological studies. Environmental Science: Nano, 6(5), 1516–1526...isothiocyanate (FITC) can be stably combined with nanoparticles, maintaining intensity at ~85% of its original...specificity to ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes ...
  5. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique in modern ...only excites fluorophores within a few hundred nanometers of the glass/water interface. Super resolution...only excites fluorophores within a few hundred nanometers of the coverslip (4). Essentially, an optical...10 fold increase in resolution, down to 10s of nanometers. Thick dynamic samples Ex: 3D cell cultures, ...KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics 48, 178-243 (...
  6. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...CRISPR/Cas9 could be used to edit several autosomal-dominant mutations that have been connected to the early...Chinese Academy of Sciences found that mice with a dominant mutation in a gene that causes cataracts could... chose to explore its potential in mice with a dominant cataract disorder caused by a single copy of a...applications are also possible through the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR...
  7. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...and the ITRs are retained with the gene in a recombinant AAV (rAAV) vector (Figure 1). Everything else...outside the ITRs gets left behind.    Figure 1: Recombinant AAV vector with ITRs that flank the gene cassette...plasmid DNA with mutated ITR sequences. Using recombinant-deficient bacterial strains such as NEB Stable...individual reads allows for identification of non-dominant ITR sequences.  Restriction digests with the enzymes...
  8. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... The binding of the GFP nanobody to its target stabilizes the TurboID-nanobody protein and allows TurboID...TurboID fused to a conditionally stabilized GFP-nanobody rather than a specific protein of interest. This...GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity of the system...
  9. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S. in accounting... on average 20 PhD’s a year and we have a very dominant presence in the biotech sector in both patent ...effectiveness of the Plan's risk management, control, and governance processes. Prior to Tufts Health Plan, Carl worked...
  10. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...others you’ll encounter include monoclonal and recombinant antibodies). By the end, you’ll have a better...polyclonal antibody, or select a monoclonal or recombinant antibody instead. Otherwise, you might experience...of background noise compared to monoclonal or recombinant antibodies. This is because the polyclonal antibody...antibodies Learn about the advantages of using recombinant plasmid-based antibodies Resources on Addgene.org...
Showing: 51 - 60 of 331 results