We narrowed to 798 results for: KIN

...the clock for others to dive in. The real cost of making data accessible A more reasonable concern with ... the scientific community is thus not averse to making data available, but there remains concern about... by others. Other new data initiatives are also taking root. Nature Publishing Group recently launched...efficient searching and extraction of information. Looking a little further ahead, journals may move towards...Instead of providing a “dead end” figure or table, clicking on the published chart could reveal the underlying...reader as possible. Rather than adding to demands, making effective data sharing the norm should open up ...Canada where he studies protein-serine/threonine kinases implicated in human diseases. His spare time is...

...scientific discovery and career development. They are working with limited resources but limitless potential....by facilitating connections to mentorship and networking opportunities, publishing assistance, and more...international scientific community and are always taking steps to increase plasmid distribution and disseminate...distribution As you can see from the chart below, we are making great progress toward spreading the Addgene community...countries. As part of these efforts, we will be working with Seeding Labs to award plasmids to research...well as shine a light on some of the great minds working in developing countries. As the world continues...

...been extremely helpful for scientists that are looking for more detailed information about this new technology...topics you want to learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular...additional questions. We’re happy to see scientists making their own cells to save money and create the tools...feature in the coming year. Finally, we are always looking for guest bloggers who want to share their research...Replication By Kendall Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids...Marcy Patrick Will You Be My Mentor? Finding and Asking for Mentoring Support By Joanne Kamens Plasmids...

...of naked DNA. Other investigators who had been working to develop high efficiency gene targeting approaches...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... CRISPR-based approaches for the generation of knockins via homologous recombination with a homologous...application - the creation of sequence-specific knockins - for which the AAV approach is most well suited...suited, since it routinely generates knockin efficiencies of 1-40% with G418-selected clones. However, there...approach requires G418 selection of infected clones, making it unsuitable for most in vivo applications.  Third... CRISPR-based approaches for the generation of knockins, it is not always ideal. Because of these limitations...

...epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment...minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds of particles...molar ratio) with 10 minutes of glutaraldehyde crosslinking: representative micrograph (C), particle orientation...could be overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated form of...compatible with the buffer additive MgCl2 and the crosslinking agent glutaraldehyde. Adding RvLEAMshort to ...j.cell.2021.03.025.    Recombinant antibodies for marking cell types in the nervous system By Ashley Waldron...filament expressed in astrocytes throughout the brain, making it a popular target for labeling these cells. Check...

... pathway. Those can include cytokines and chemokines; enzymes like kinases, phosphatases, and ubiquitin...false positive signal. Dump gating When you are working with heterogeneous cell mixes, like bulk lympho... buffer solutions. Fixing causes protein cross-linking, killing the cell but preserving the cellular state...gating uses the same color for different markers, making it easy to gate on the negative cell population...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

...in vitro kinase reactions. Often, however, researchers lack knowledge of the regulatory kinase, access ...access to active kinase for the reaction, or have difficulties with kinase specificity in vitro. The establishment...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...phosphoserine into recombinant proteins, such as the kinase MEK1 (5). MEK1 is inactive without requisite dual...create a pSer/phosphomimetic dual mutant) elevated kinase activity over 70-fold over dual phosphomimetic ...

...every day, sharing ideas for the company, but also talking about sports, movies, and just about anything. ...cite lunch as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can...Chef-style competitions Every few months, we’ll have a cooking competition featuring a selected ingredient. Anyone...cook-off last week, and the featured ingredient was pumpkin. Everyone gets to vote for their favorites, and...the stiff competition or says something about my cooking.   This is a creative (and delicious) team building...work better and keeps us smiling even when we’re packing the 400th plasmid that day.   As we head into Thanksgiving...

...Does this sound familiar? Well, when it comes to working with Addgene, we certainly hope not! One of our...email. You need help right at the moment you’re looking for it, or you may just prefer to troubleshoot ...icon, search for answers, and choose a result. By clicking on the result links themselves right from within...ll navigate to articles answering your questions making it easier than ever to get the support you need...some reason you can’t find the answer that you’re looking for, or you just have a general question or concern...valuable feedback. This is Addgene after all, and making your research easier always comes first. Resources...

...have an opportunity to improve my networking with mentors who are working in USA and Europe, something that...who migrated from their country some years ago looking for their career improvement) and bright students...world in flux. The professional development and networking resources offered by programs like IMP USA-Europe...Institute of Technology, Boston University, Johns Hopkins University, the Salk Institute, and the Karolinska...and contacts with Spanish laboratories to start working on projects together. List of discussion topics...Addgene Read Other Career Blog Posts Read the Networking for Scientists Series Read our Blog Post about...

...its value. My master’s strengthened my critical thinking and problem solving abilities, increased my technical...writer,” among others. Don’t discount job postings looking for PhD applicants since many will also accept ...people describe their actual work. To do this, try looking at LinkedIn profiles of people who have masters...Is their career path one you could see yourself taking? If so, consider setting up an informational interview...degree prepared them for their career.  2. Are you looking for career advancement and growth? A master’s is...project.  3. Do you prefer being a generalist? Working on a variety of projects and in different fields...

...fused to calmodulin (CaM) and the chicken myosin kinase calmodulin binding region RS20. When calcium is...place for the biosensor to be expressed. Are you looking at an intracellular or extracellular molecule? ...extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end ...with your lab and experimental setup? Consider what kind of instrumentation you have available, and the conditions...and multi-color imaging experiments. Finally, what kind of system will you use to detect your fluorescent...method depends on what type of information you are looking for. Below we have summarized some of the common...

...equipment acquisition, public speaking and personnel management?  If you are thinking about a non-academic path...advisors learning how to be a scientist. It is shocking how little pre-work most PhD students and postdocs...learn how to be a scientist. Your PI should be the kind of mentor that will teach you how to: interpret ...with the potential advisor and don’t be shy about asking hard questions. Talk to as many lab members as ... time with everyone. Additionally, if you are looking for more resources to help you choose a good mentor...

...Welcome to our Summer SciComm Series, where we’ll be talking about science communication, best practices in ...science communication research.   This summer, we’re making time to talk about my favorite thing - science ...but the foundation of all good communication is making sure everyone’s on the same page. So for our first... endless.    Activity Example audience  Talking about your career as a scientist underrepresented...enjoy listening to podcasts A series of Tweets breaking down a new publication that could advance treatments...Communication - 9 Ways to Get Your Foot in the Door Taking Science to Elementary and Middle Schools   Image...

...cell membrane.  Picking the Right Antibody  It can get even more complex if you’re looking for proteins ...keep epitope availability in mind whenever you're working with antibodies.) Conformation changes usually ...Processes  If you’re feeling relieved that you’re working with native proteins instead of denatured ones ...are interacting with one another, or if you’re looking for a protein of interest while it’s in a transition...validated for ELISAs, it has a good chance of also working for a Western because they both typically use denatured...

... sponsors a global competition for researchers working on rare diseases, offering them the chance to win...Claudio Aguilar (right) at Purdue University and is working to understand Lowe Syndrome, a devastating genetic...great for us,” Wang said. “We were planning on and looking at a bunch of different strategies with CRISPR-...hearing loss and changes in coloring of the hair, skin, and eyes. The researchers found that seven patients... budget is approximately 20,000 USD and we are working on several genetic diseases at the same time. The...studies.” Thank you to all the BeHeard Awardees for speaking with us! Additional Resources Find out more ...

... to email strangers asking to meet. I have cold emailed hundreds of people asking for meetings to discuss... carry a heavy price tag, especially if you're working on something other than software.  If you are a... your entire career in science, you are likely lacking the necessary business background and/or connections... Start with your network You've spent your life making connections and finding mentors -- well, it's time...discuss each project and business decision before making it, to have an internal review system.  On top ...

... and high resolution functional tracking of T lymphocytes, too, making them a versatile tool for application...Max Planck Institute for Neurobiology has been working on genetically encoded indicators of calcium and...has a particular advantage for observing events taking place inside cells that are on the move, such as...element-binding protein (CREB) in living cells. “Now we are making this really good,” Griesbeck said. “We know CREB...genetically encoded magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start...

... optogenetic tools in a deep part of the brain, making it sensitive to light. When they illuminated this...patients? Over a quarter of a million people have some kind of neural implant already. If we can use light to...maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other conditions.... emotions, to see how a monkey changes decision making, to cure a human patient. We can't do it all by...you have any special tips or tricks for someone looking to try these optogenetic tools out for the first...always tell people, 'Be driven by your science.' Looking for Optogenetics Plasmids? Plasmids for Optogenetics...

...gene edit. I wanted to inactivate the gene BRAF (a kinase implicated in several human cancers) in A549 cells...Vinculin is a loading control. 3. In terms of making the monoclonal line: I had never done it before...found here. Figure 3: A surprise for the author. Checking Cas9 expression Overview: By Western blotting ...difficult part of the assay was designing primers flanking my edits in genomic DNA and doing PCR on genomic...agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene knockout was straightforward...who you are, you could quickly be on your way to making new scientific observations. References 1. Rago...C, Vogelstein B, Bunz F. Genetic knockouts and knockins in human somatic cells. Nature Protocols 2007;...

...something difficult, be excited about the prospect of working toward a solution. At the end of the meeting, make...person to add a bullet or two about what they are working on. Building this into the agenda ensures that ...items, and Accountability Agendas: Send around talking points for the meeting a day in advance. This gives...Accountability: At your next meeting, start by checking in on the action items from last meeting. Use ...project moving. Use time wisely Meetings are for talking, not reading: Send around relevant documents, like... Executive Director Joanne Kamens FInd Tips on Picking a Research Project Read Other Inside Addgene Posts...

...the day you defend to start thinking about your next step. Start thinking now. Start browsing job postings...in your resume. If the job posting says “French-speaking is an asset,” mention “speaks French.” Employers...groups? Perhaps you had some supervisory experience working with undergraduate students? On top of that, perhaps...universal. Some universities even have officers working specifically to assist graduate students in job...postings and salary expectations public, so start by looking at these university postings to give yourself a...

...you build your lab team and foster an effective working environment? Tom Ellis was offered a start-up package...ideas, and ways to improve the lab. “An effective working environment for research is totally dependent on...communication amongst the team so getting everyone talking to each other cannot be undervalued.” Connie Cepko...of slowly building up your lab and selectively picking the first couple of hires to build a strong, collaborative...resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc... Dr. George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists...

... these files to your working directory (the folder that you are currently working in) every time you use...notebooks just won't cut it for bioinformatics. All kinds of biologists are finding themselves using computational... task will keep you from repeating your work or making similar mistakes in the future. Stay organized...comment in C++ and Java Command line history: Tracking command line history by recording all commands...system that does is through trial and error, and by asking for tips from others. Comment below to add your...

...Blog Mentoring for Scientists Series. I have been thinking a lot about Mentoring for over 10 years. Many ...Cepko to learn about her mentoring style. While working on this project, I read the book “Every Other Thursday...Form your own peer mentoring group In my travels speaking to grad students and postdocs I have started to...example, a group of people who are all interested in making a transition. Schedule your first meeting and get...breakfast once a month. All participants must commit to making the meetings a scheduling priority (and last minute...career paths and get their advice. Attend a local networking event together and help each other practice meeting...

...like multi-color imaging (Figure 2). If you’re working with purified proteins in vitro, you can even use...tags makes updating your labels easy! Instead of taking the time to clone a new construct for every new-and-improved...time points.   Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins...developed over the years; improving the labeling kinetics or the fluorophore’s microenvironment to enhance...A., Juillerat, A., Heinis, C., Corrêa, I. R., Kindermann, M., Beaufils, F., & Johnsson, K. (2008). An ...Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling...Selecting Fluorescent Dyes and Ligands GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic...

..., consider making you own DNA ladders with these plasmids from Penn State Need help picking the right ...cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell culture media. Their...HA staining is weaker than reporter staining. Picking the right reporter for your RANBody While all three...staining GFP, RFP, H2A2B   Are you ready to start making your own RANbodies? Great! All of the RANbody plasmids...learn more about nanobodies Additional Resources Looking for primary monoclonal antibodies to use with the...

... Research. On December 1st 2016, many postdocs working more than 40 hours per week could expect to see... salaried workers receive overtime payment for working more than 40 hours per week increasing from $23,660... postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...going ahead with their planned raises, some are taking the opportunity of the lapsed mandate (or are being...University of Massachusetts Medical School. We are working with postdocs at other institutions to see if we...that many postdocs already made plans and budgets working around issues such as childcare using money from...

...heavily with other skills such as critical thinking, decision making, creativity, or data analysis. Conveniently...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...you spend time in a lab learning techniques and taking on independent projects, you will inevitably develop... skills passively. But I have always found that taking a conscious or active approach can be hugely helpful...are, while also practicing systematic, logical thinking.  If you are already a lab veteran at this point...

... be useful. Mainly because, well, antibodies are kind of hard to see. To solve this issue, researchers...post, where you can find some additional tips for working with these tools.          Enzymes An equally, ...properties allow for efficient signal amplification, making biotin conjugated antibodies good options for detecting...lead perfectly blissful, productive lives without thinking about these chemical reactions. After all, there... are ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...antibodies’ performance after the reaction.  If you are looking to conjugate antibodies yourself, there are kits...