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Showing: 201 - 240 of 507 results

  1. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that...Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio, a system...

  2. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and...

  4. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

  6. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much...

  7. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and ... Tsang, Science Communications and Marketing Coordinator Non-science favorites  The Mistborn series by...

  8. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...skills include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor..., including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...bodies do for you? R bodies are robust linear actuators that could be used in a variety of applications...applications, purified R bodies could also perform as actuators in microelectromechanical systems (MEMS) devices...

  10. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...37825  AAV1 pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472  AAV5, AAV8 pAAV-...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120  AAV1 pAAV-ihSyn1...

  12. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry, microbiology...the time I was ready to graduate with my BSc in Anatomy, the University of Otago had established the Centre...

  13. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation...

  14. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...

  15. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...will choose to start their own academic research laboratory. How does one even go about starting a lab? Obviously... does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager, researcher, adviser...

  18. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...

  19. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...respectively, act as RNA polymerase II promoter and terminator regions and are slightly different in sequence.... Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector ...

  20. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ... Expression Low Tropism for Mouse Cells and Hematopoietic Cells Low Packaging Capacity (~4.5kb From...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed...

  21. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website...graphic designer upon request that the science communicator "just sketch a western blot out quickly." Final...

  22. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...by the fusion of Cas12f with a transcriptional activator, CasMINI is able to induce high levels of gene...

  23. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...notice within 24 hrs, send a note to the program coordinator.   6. Virtual interview prep Prepare for your...

  24. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... sequence (“microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of...JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided...

  25. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ..., one that is not food, not medicine, and not mandatory – people can decide for themselves if they want...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside...

  26. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...your sequencing results back, you can use the chromatogram to spot whether any discrepancies between your...

  27. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...

  29. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains contested...counselor, Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research Associate...

  30. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...our curated list of biosensors too, as another laboratory may have already created the sensor that you ...PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based on green fluorescent proteins ...

  31. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first...

  32. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...this study laid the foundation for how different anatomical regions of the brain receive inputs and compute.... 5. Nassi JJ, Cepko, CL, Born RT, Beier KT. Neuroanatomy goes viral! Front Neuroanat. 2015 Jul 1;9:80...

  35. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...open worm browser, which allows you to view the anatomy of worm layer by layer, the NeuroML C. elegans ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ...

  36. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section...

  39. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...human tRNA genes and, until now, we've overlooked regulatory fragments of these transcripts. I remember when...Medicine presented another unexpected finding in the regulatory RNA field. The Rajewsky lab is studying circular...

  40. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...protocol, the size of the cuvettes your lab’s electroporator uses, etc. The DNA amount can be anywhere from...Screening Zhang Lab - Multiplexed Overexpression of Regulatory Factors (MORF) Library 3,548 250 - 10,000...
Showing: 201 - 240 of 507 results