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... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science...applied and I got it.” Science writing at Fred Hutchinson Cancer Research Center Work as a Science Writer...as you can to get your name on things.” Susan personally found this easier to do while still in school...

...Abrahrnsen L, Oesterlof B, Josephson S, Oestling M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale...

...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies... toxic protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded...killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol...

... of tea, and you are more of an audio or visual person. Why don’t you record a podcast or put some effort...yourself as an educator for a short, animated TED-Ed lesson. 6) Network – Mingle with pros Where do you meet...Communication. In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication...

... both NeuroMab and the Allen Brain Atlas. The comparison with two atlases enables us to see the reproducibility...literature suggest, providing an expectation and comparison on the antibody signal’s location. If a distinct... discuss the results together, explaining our reasoning to determine if the antibody is negative (poor...

....34870.  Kaas GA, Zhong C, Eason DE, Ross DL, Vachhani RV, Ming G-L, King JR, Song H, Sweatt JD (2013) TET1...linked to disease has now become much like the 1980s song “Don’t Stop Believin’”; whenever you hear it (whether...

...at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level...General viral handling safety   Containment and personal protection are key to lab safety and the same ...reduce the risk of an accident (the most common reason for lab accidents is not following safety protocols...

...spike protein, called HexoPro, was developed by Jason McLellan’s lab. This version of the spike protein... Lee AG, Liu Y, Chou C-W, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, ...Seaman MS, Schmidt AG, Chen B, Neuberg D, Bajic G, Harrison SC, Wesemann DR (2021) Memory B cell repertoire...

...Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, ...piece of music. It was tough to share or remix the songs, the CDs could get scratched over time, and it was...

...to non-scientists and may make your perfectly reasonable paragraph unintelligible to a lay audience. ...HR on the judging panel. It’s important for this person to get a clear and concise explanation as to why...Writing Professional Facilitate Career Planning and Personal Development with an IDP Get Tips on Keeping Up...

...PMID: 14723844. 3. Atasoy D, Aponte Y, Su HH, and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin...PubMed Central PMCID: PMC2593125. 4. Saunders A, Johnson CA, Sabatini BL. 2012. Novel recombinant adeno-...Wenbiao Chen Liquin Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui...

...adolescence passed on a heritable trait to their grandsons that decreased their likelihood of death from ... Ong-Abdullah, Meilina, et al. "Loss of Karma transposon methylation underlies the mantled somaclonal .... 3. Kaati, Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined...

...slightly if you know a bit more about the other person. If you have a poster or talk at the conference...there parts of your elevator pitch where the other person seemed confused, bored, or particularly excited...after. For one conference I went to, I made it a personal goal to ask one question each day (this may be...

...., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants, B. W., & Robinson, J. T. (2019).....4124.  Kattnig, D. R., Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel...

...recommend using a Tris-based lysis buffer and sonication.  The obtained virions from cells and supernatant....2020.120 Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination of single-stranded...L. H., Xiao, R., Lock, M., Lin, J., Korn, M., & Wilson, J. M. (2010). Efficient serotype-dependent release...

...efficiency. Figure used with permission from the Mark Dawson lab Re-transformation of the SPLINTR Libraries ...recombined backbones that may replicate faster. For this reason, if your library has inserts of different sizes... Hannah Dotson...

...is very tricky to use and fussy to optimize. One reason could be the instability of the supposedly alien...many may find this inconvenient. Another possible reason for the trickiness of NgAgo is the inability of... not snipping DNA, he tries random things (101 lessons on Spanish, C++, guitar, photography) and often...

...next thing. If I made a mistake, I evaluated what lessons I had learned – call it “troubleshooting.” Trying...I asked some colleagues to share valuable life lessons they have learned during their scientific training... them in a blog post on Born to Science: Life Lessons from Scientific Training More and more voices ...

...Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74... Ross A, J. Hecksher-Sorensen, R. Baldock,  D. Davidson. (2002) Science, 296, 541-545. Pubmed. A. Ertürk.... Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et al. (2013) Nature, 497, 332-337. Pubmed. R....

...gross comparison of AAV.PHP.eB and AAV.PHP.S to their parental vectors. To quantify this comparison, capsids...PubMed PMID: 28671695. 2. Deverman BE, Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants...

...Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease of transformation...a protocol for antibiotic selection, check out Harrison et al., 2006. Tips: Don’t plate seeds too densely... 24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method...

...the statistical independence assumption of the Poisson process. This modification also eases the interpretation...orders.  Our model factors the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t)....contains an intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects...

...genes that apparently contribute to virulence (Tollefson et al., 1996) – cell death has been reported when...6301263. Servellita, V., Sotomayor Gonzalez, A., Lamson, D. M., Foresythe, A., Huh, H. J., Bazinet, A. ...10.1182/BLOOD-2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, ...

...qPCR runs before a new one is needed. For these reasons, we are in the process of transitioning our titering...for the detection of low copy numbers. For this reason, it can be used in the detection of rare sequences...PMID: 24328707. PubMed Central PMCID: PMC3991984. Song, Neng, et al. "Detection of circulating Mycobacterium...

...vertebrate embryogenesis. To illustrate some of the reasons that zebrafish have been embraced as a model organism...examine the consequences of manipulating genes. One reason for the success of zebrafish as a model organism... 2016). One common and robust strategy uses a transposon called Tol2 to randomly integrate foreign DNA...

...Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity.... Kim E, Koo T, Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim ...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...

...mammalian cell lines. However, in those same comparisons, the AbuGoot lab reported no detectable effect...and can be easily programmed for new genes. In comparison with other large insert techniques like HITI ... Rachel Leeson...

...screening! This blog was written by Addgenie Hannah Dotson.  Resources and references References Genome-...protocols.  References McDade JR, Waxmonsky NC, Swanson LE, Fan M. Practical Considerations for Using Pooled...  Hannah Dotson...

...meeting who felt isolated at a conference for any reason was a Singleton. Figure 1: The Binning Singletons...Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric Griffis, Janet Iwasa,...Dunivin, Jen Greenwich, Susanna Harris, Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg,...

... can arise, and has arisen, for many different reasons. Exposure to antibacterial drugs provides a selection...households by observing proper cleanliness and personal hygiene standards. Handwashing is an essential...spread of antibiotic resistant bacteria from one person to the next. For more information and tips on preventing...

...lack of documentation and high turn-over of lab personnel. If you are unsure of the background of the lines... such as serum, other cell lines, or infected personnel and can persist undetected; unlike infections ..., Kassem H.S., Pepper S.D., Hey Y., Ward T.H., Margison G.P. “Mycoplasma infection significantly alters...

... a fluorophore (Figure 1).   Figure 1: Comparison between how direct immunofluorescence and indirect... will focus on this technique here. One of the reasons that indirect IF is more common is that secondary...primary, resulting in signal amplification. Another reason is cost effectiveness. Generating and validating...

...samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be damaged at the air-water...  Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M....Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021). Genome-wide...

...different projects, you might not be able to make any reasonable progress on any one of them. That’s why, as I...recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do lists. Because...Bored and Brilliant” for learning more fantastic reasons to remove yourself from distractions every once...

...be quantified by the strength of the signal in comparison to a control sample. Southern blotting applications... sequencing of many samples at once for later comparison. These next-generation sequencing techniques ...Sanger, Frederick, Steven Nicklen, and Alan R. Coulson. "DNA sequencing with chain-terminating inhibitors...

...being mentally challenged. For these and other reasons, I started  looking for other career options after...government loans to study a MSc, so I had to take out a personal bank loan. I saved money from my role in Industry...or another country of the Commonwealth. Another reason why I chose Australia was because it is an English...

...cross country boundaries for both professional and personal development, is essential to excelling in the ...adapt their mentoring styles to the mentee’s personality and professional needs at any time. In its Pilot...advisor. For their own part, mentors receive immense personal gratification as they witness their student’s ...

... were suitable for our needs for the following reasons: Safety: Since AAVs are nonpathogenic, we are...Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors (i.e. chemogenetics...applications, we may also be able to use these comparisons to enhance our understanding of receptor physiology...

...better plan your transductions and use a range of reasonable MOIs. 2. Create a monoclonal Cas9-expression ...used existing BRAF gRNA viruses. There are a few reasons to target different regions of the gene with multiple...testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months to knockout a...

...commonly used for recombineering can be found in Thomason et al and Sharan et al (a few strains can also...ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL. Recombineering: A ...19180090. PubMed Central PMCID: PMC2790811. 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N...