We narrowed to 291 results for: Fus

...construct is inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific...

... single point mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these...

... bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin...

...inquiry – purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and...

...sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining...

...directed binding site. These methods include the fusion of an activation domain such as VP16 to dCas9 or...

...Cas9 expression is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic...

... Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...

...Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined...

... containerization, but haven’t tried it or are confused by the concept, don’t worry–you are not alone!...

...envelope protein causes cells that it infects to fuse together and form syncytias that cause the cells...

...also small enough to deliver with AAV. This system fuses catalytically dead dCas13b to the catalytic domain...

...Editing for Programmable A to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific...

.... They don’t simply rely on chance (or random diffusion) to make this happen.  There are several different...

...might be calling it an aliquot. This can lead to confusion as to what the code is supposed to be doing. When...

... Our Stem Cell Collection Transcription Factor Fusions for Enhanced Reprogramming Find plasmids from the...

...e.g. GFP) can be used in many ways. Often a FP is fused to a protein of interest to elucidate the subcellular...

...economic importance as harmful weeds. The parasitic fusion is such that the two plants share RNA, proteins...

...Specifically, SHH is a morphogen, a chemical signal that diffuses down a concentration gradient exerting a different...

...for the upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity...

...the microinjection was done as the pronuclei were fusing, you may have several mosaic mice. To figure out...

... combining Cas9 DNA-targeting technology with a fused cytidine deaminase to create C→T base edits at a...

...visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely, while others ...

...antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All bind specific ...

...answer an expert's question than to leave everyone confused about your research because you waded too deep...

...taking care of that”, because that can indicate confusion around... Ownership: Misunderstandings about who...

...less interference with the oligomeric state of a fusion partner. Therefore, we recommend using miRFPs and...

...tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including Xenopus. ...

...templates for applications that require large gene fusions. The current state-of-the-art in combining the ...

... composed of a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence is observed...

...available from Addgene. This system uses the dCas9-VP64 fusion commonly used to activate transcription, but with...

...techniques, such as Golgi staining, injection of diffusible labels, or electron microscopy of stained sections...

... antibody against your target protein, consider fusing a protein tag to the target and using an epitope-based...

...actually performed, potentially curtailing future confusion (and hours sifting through paper lab notebooks...

... of the skyline of Acadia National Park. I was confused. It was supposed to be hard. I was mentally prepared...

...axonal transport desirable or not desirable? If diffuse gene expression is tolerated, then axonal transport...

...minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF...

...stimulus. Using an epifluorescence microscope and GFP fusion proteins controlled by promoters specific to the...

...coli The Kondo lab expressed a cytidine deaminase fusion with Cas9 with a uracil DNA glycosylase inhibitor...

...genetic material. Proteins are delivered to cells by fusing them with peptides that help mediate their transduction...

...150 µm to image a blot. This is because proteins diffuse semiradially during the transfer, making membranes...