Skip to main content
Addgene
Showing: 251 - 282 of 282 results

  1. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...might be calling it an aliquot. This can lead to confusion as to what the code is supposed to be doing. When...

  5. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...for the upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity...

  6. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...the microinjection was done as the pronuclei were fusing, you may have several mosaic mice. To figure out...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... containerization, but haven’t tried it or are confused by the concept, don’t worry–you are not alone!...

  11. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...envelope protein causes cells that it infects to fuse together and form syncytias that cause the cells...

  12. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely, while others ...

  13. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All bind specific ...

  14. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...answer an expert's question than to leave everyone confused about your research because you waded too deep...

  15. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... composed of a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence is observed...

  18. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including Xenopus. ...

  24. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ... of the skyline of Acadia National Park. I was confused. It was supposed to be hard. I was mentally prepared...

  27. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...axonal transport desirable or not desirable? If diffuse gene expression is tolerated, then axonal transport...

  30. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...coli The Kondo lab expressed a cytidine deaminase fusion with Cas9 with a uracil DNA glycosylase inhibitor...

  31. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...genetic material. Proteins are delivered to cells by fusing them with peptides that help mediate their transduction...

  32. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...150 µm to image a blot. This is because proteins diffuse semiradially during the transfer, making membranes...
Showing: 251 - 282 of 282 results