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Showing: 101 - 120 of 390 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ... colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia virus DNA topoisomerase...coli is sequence specific."  Proc Natl Acad Sci U S A.  1991 Nov 15;88(22):10104-8. PubMed PMID: 1658796...synthesis using vaccinia DNA topoisomerase. Shuman S. J Biol Chem.  1994 Dec 23;269(51):32678-84. PubMed...

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H., Lim, Z. F. S., & Ng, S. K. (2021). HEK293 cell line as a platform...Plasmids and Resources References Shaw, G., Morse, S., Ararat, M., & Graham, F. L. (2002). Preferential...

  3. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...26225354. PubMed Central PMCID: PMC4515362 Robinson, W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid...Rous sarcoma virus: Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology.../0022-2836(73)90277-5. PubMed PMID: 4355596 King, S. R., Duggal, N. K., Ndongmo, C. B., Pacut, C., & Telesnitsky...18094172. PubMed Central PMCID: PMC2258930 Hu, W.-S., & Temin, H. M. (1990). Genetic consequences of packaging...

  4. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... et al. used a phagemid system to target virulent S. aureus, a common hospital-borne infection. Two rounds...phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just 24 hours.   ... to target the sensitive population. Yosef et al.’s proof-of-concept work targeted two distinct beta-lactam...antibiotic-resistant bacteria.” Proc Natl Acad Sci U S A. 9;112(23):7267-72. (2015). PubMed: PMID 26060300...

  6. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...Genesis 34, 1–15 (2002).PubMed PMID: 12324939. 3. S. E. McGuire, G. Roman, R. L. Davis, Gene expression... PMID: 21356876. 5. J. Chow, Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress Causes Lethal...PMID: 22205518. PubMed Central PMCID: PMC3574576. 7. S.-L. Lai, T. Lee, Genetic mosaic with dual binary transcriptional...

  8. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ... data only, information on requesting PI(s) & organization(s) is not shown on the dashboard. Key features...

  9. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...861-72. PubMed PMID: 18035408. 3. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from...temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234-9. PubMed PMID: 21821793... of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848-57. PubMed PMID: 22645348...10): p. 1836-46. PubMed PMID: 22976355. 34. Wang, S., et al., Differentiation of human induced pluripotent...p. 82-94. PubMed PMID: 23329641. 36. Jopling, C., S. Boue, and J.C. Izpisua Belmonte, Dedifferentiation...): p. 987-1000. PubMed PMID: 3690668. 40. Ferber, S., et al., Pancreatic and duodenal homeobox gene 1 ...

  10. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Powers S, Miranda C, Dennys-Rivers C, Huffenberger A, Braun L, Rinaldi F, Wein N, Meyer KC, Solano S, Nguyen....cell.2013.01.038 Guy J, Gan J, Selfridge J, Cobb S, Bird A (2007) Reversal of Neurological Defects in...Sinnamon JR, Kim SY, Fisk JR, Song Z, Nakai H, Jeng S, McWeeney SK, Mandel G (2020) In Vivo Repair of a ...

  11. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...PubMed Central PMCID: PMC3193421. 4. Hacein-Bey-Abina, S., et al., LMO2-associated clonal T cell proliferation...5644): p. 415-9. PubMed PMID: 14564000. 5. Seki, T., S. Yuasa, and K. Fukuda, Generation of induced pluripotent...(4): p. 718-28. PubMed PMID: 22422317. 6. Diecke, S., et al., Recent technological updates and clinical...transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010. 107(32): p. 14152-7. PubMed PMID: 20660764...

  13. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could use information...target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki...activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena...
Showing: 101 - 120 of 390 results