...’t have to worry about having enough space! There’s enough space here at our new location to grow. We ... welcoming, inclusive workplace for everyone. We had Unbiasing Training in place before it was cool or...supportive Board, we have a robust committee process in place and continue to incorporate additional Board best... hard but as a 3 year Boston Globe and BBJ Best Place to Work with a positive, supportive culture, we ...

...other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, ...they are not currently provided. Eye protection or face shields are required whenever there’s a splash or... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ...decontaminate after use. Virus-exposed plastics and other surfaces should be thoroughly decontaminated after use,...basically, you’ll need to get trained in each new place (and pay attention to the trainings!), even if you...

...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine... GFP on the surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells...cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells. What’...

...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...research, and prevent technological advances from exacerbating existing inequalities and injustices. The Personal...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....quickly into sensitive topics and uncomfortable places, from sticky ethical dilemmas to dark episodes ...policy requires broad interest in issues at the interface of science and society, and an ability to talk...in the legal, ethical and policy issues at the interface of biology and society.     Additional resources...

...target cell line, researchers often find themselves faced with a roadblock. In this post, we will provide ...concentrated by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin...starting. We recommend calculating the ratio of the surface area of the larger vessel to the 10 cm2 dish and...some final adjustments, this is generally a good place to start. If you don’t anticipate that you will ...relatively inexpensive and does not require an ultracentrifuge, PEG will precipitate all proteins in a solution...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

... by ultracentrifugation (75,000 - 225,000 g for 1.5 – 4 hours at 4 °C). After ultracentrifugation, virus...vectors) you can concentrate your virus by ultracentrifugation of the viral stock, followed by resuspension...packaging cell debris before proceeding to ultracentrifugation to avoid contaminating your transduction ... to concentrate a virus solution without ultracentrifugation, you can reduce the volume of cell culture...exchanging the medium after transfecting cells, replace the medium with half the volume typically used ...

... Specially constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous...targeting takes advantage of this natural process to replace a targeted genetic locus with homologous sequence...gene will be removed from the chromosome and and replaced by the resistance gene.The gene is thus disrupted...recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted gene is disrupted ...normally because one of its exons has simply been replaced with the same sequence flanked by LoxP sites while... while the resistance cassette has been placed into an intron.    Figure 4: A targeting vector...

...student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will...Clutter not only makes it difficult to clean your workspace properly, but can also disrupt the laminar air...materials into the hood. This will give you enough space to move around freely without causing spills. Also... than 80-85% confluent because cells like their space. If they reach confluence, they stop growing (contact...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology...

... they not only traced the 3-D path of the protein’s backbone in three-dimensional space, but their flexible...ribosomal subunit, he kept sliding his palm across the surface of the protein where the nascent polypeptide emerges...realized how perfectly flat the complex is on this surface”….where the complex is docked on the membrane of...serious way. We represented the Cas9 protein in a surface format made of rock-hard, inflexible plaster. As...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...

...in-house software has been crucial for us to keep pace with our current need to analyze nearly 300 sequences...sequencing data collection and analysis eventually outpaced our software, even with continuous updates. The...other bioinformatics purposes  Integrated user interface, so our QC team could initiate and monitor the...open-source sequence assembly tools at the appropriate place in our workflow without having to translate or convert...Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with the basic...smoothly operate the pipeline, we needed a user interface. In our next post, we’ll share our story about...

...of common genetically encoded neuronal tracers Neuronal tracers such as epitope tags and fluorescent proteins...Spaghetti Monster - A new and improved neuronal tracer With the limitations of epitope tags and fluorescent...fluorescent proteins, it has been crucial to develop new tracers that enable accurate labeling of neurons. As discussed...Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins such as GFP have been used...labeling experiment using smFPs and standard neuronal tracers. 2. Visualization of neuronal sub-cellular structures...

...simply embellish and adorn your lab space or your work from home space in a fun, festive holiday or winter...winter or holiday theme decor in your work from home space… but with a science twist. Maybe you have a collection...using life science catalog pages. Make your WFH space feel a little more festive this holiday season! ...Instagram as we share pictures of our decked out spaces.       ...

...non-scientists alike will march to advocate for science’s place in education, government, and civilization in general...attribute that enables science: “humility in the face of evidence” (4). Frustration among scientists has...aspects of research at National Aeronautics and Space Agency, the Environmental Protection Agency, and...inclusion of science) they come from many different places supporting many different positions. It would be...and 514 satellite marches are scheduled to take place around the world. There is no requirement that marchers...plan Science Magazine: NIH, DOE Office of Science face deep cuts in Trump’s first budget Environmental ...

...information is consolidated to one place, easily retrievable, searchable and traceable. However, switching from ...that bring more to the table in terms of data traceability and reproducibility of research. Electronic ... enables researchers to store everything in one place. From pictures to excel sheets, researchers today...enable researchers to keep all their data in one place and avoid scattering intellectual property across...run, ELNs enable labs to keep their data in one place and therefore make it easier for them to track and...

...(Brinkman et al., NAR). This technique uses the trace file generated in Sanger sequencing and a simple...Fig. 1: Overview of TIDE. The input is sequencing trace files of WT and edited cell lines. Then decomposition...and your Cas9-targeted cells. Simply upload both trace files to the TIDE tool online along with your sgRNA...outlined above), except that it uses a 3rd sequencing trace file from the donor DNA. This method can precisely...edited sequence would look like (the 3rd sequencing trace). While it requires a little extra wet lab work,...quantitative assessment of genome editing by sequence trace decomposition. (2014) Nucleic Acids Research Volume...

...versions obsolete, the techniques we use in lab are replaced by improved versions that save both time and money...observed. Today Southern blotting has been largely replaced by real-time PCR to answer the same experimental...unknown sequence. In the era before sequencing was commonplace, researchers used restriction mapping to investigate...this technology, radiolabelled nucleotides were replaced with fluorescently labeled ones allowing for fluorometric-based...discoveries (5, 8). While newer techniques have largely replaced these, these techniques still have useful applications...to recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly...

...world continues to face global challenges, we hope to empower those that need to face these challenges ...global challenges. However, these same researchers face significant barriers to scientific discovery and...near future, we hope to eliminate much of white space on this map and increase plasmid sharing across ...Follow Seeding Labs on Twitter @SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn...

...interesting, much of what a phage faces as it approaches a host cell surface are complex polysaccharide structures...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...colonies overnight), flip the membrane over and place it colony-side up onto a fresh LB plate containing... is done, peel the membranes off the plates and place each onto a lysis solution-soaked filter paper (... blocking solution (around 108 CFU/mL is a good place to start) and add your membranes to the solution...

...your Addgene account.  Orders do not need to be placed immediately upon request approval. Once approved...request will remain in your account until you have placed your order. You will only be charged once you have...  What happens after I order?  Once an order is placed, the Addgene viral vectors team gets to work! They... before you order.  Timeline Once your order is placed, we estimate it will take 6-8 weeks for the service... you’ll be able to work with the distributor to place a request and order if approved.  I want to stay...

...researchers from different locations. Having virtual face-to-face meetings as opposed to phone calls most closely...feel included in the project and accelerates the pace at which problems can be addressed and new ideas...that work best for them. This also accelerates the pace of research by circumventing the slower, sequential...Scientific conferences and workshops are another great place to meet up with collaborators. Being able to present...