We narrowed to 612 results for: ACE

...choose, whether it's tweaking the antibody-antigen interface or fine-tuning the regions you want to evolve....high-throughput screening techniques such as ELISA and cell surface display are common. ELISA enables quantitative ...comparing relative affinities across mutants. Cell surface display systems, such as bacterial, yeast, or mammalian...the presentation of your mutant libraries on the surface of cells. This enables direct screening via flow...recombinant antibodies that target families of cell surface and secreted proteins (glypicans, integrins, and...H., Lax, B. M., & Wittrup, K. D. (2022). Yeast Surface Display for Protein Engineering: library generation... M., Manglik, A., & Kruse, A. C. (2018). Yeast surface display platform for rapid discovery of conformationally...

... entered human cells via hACE2, could not enter cells expressing murine ACE2 (Subbarao et al., 2006). ...SARS-CoV-2. The resurgence in popularity of the hACE2 transgenic mouse strain, for example, is sustained...the University of Iowa (McCray et al., 2006), the hACE2 transgenic mice were designed after it was discovered...Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing logistic hurdles...Look DC, Perlman S (2006) Lethal Infection of K18-hACE2 Mice Infected with Severe Acute Respiratory Syndrome...

... that helps attack extracellular pathogens.  Transportation of antibodies to places they can’t reach on...valency of 10 antigens. An extra constant domain replaces the hinge region in IgM antibodies. IgM has no.... IgD While IgD is coexpressed with IgM on the surface of most immature B cells, its function is unknown.... They are found bound to IgE receptors on the surface of basophils and mast cells, two types of white... the intervening DNA sequence is deleted, which places the variable region next to a different constant...

...target proteins. The surface-exposed loops of nanobodies seemed like a good place to put AsLOV2 because...inserted AsLOV2 into conserved sites in all eight surface-exposed loops of an mCherry nanobody. Only insertion...-dependent shift in mCherry fluorescent to the surface of the bead, suggesting that OptoNBs still bound...bound their target protein even when stuck to the surface of a bead.  Using OptoNBs to control cell signaling...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade...

...’t have to worry about having enough space! There’s enough space here at our new location to grow. We ... welcoming, inclusive workplace for everyone. We had Unbiasing Training in place before it was cool or...supportive Board, we have a robust committee process in place and continue to incorporate additional Board best... hard but as a 3 year Boston Globe and BBJ Best Place to Work with a positive, supportive culture, we ...

...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine... GFP on the surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells...cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells. What’...

...other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, ...they are not currently provided. Eye protection or face shields are required whenever there’s a splash or... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ...decontaminate after use. Virus-exposed plastics and other surfaces should be thoroughly decontaminated after use,...basically, you’ll need to get trained in each new place (and pay attention to the trainings!), even if you...

...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...research, and prevent technological advances from exacerbating existing inequalities and injustices. The Personal...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....quickly into sensitive topics and uncomfortable places, from sticky ethical dilemmas to dark episodes ...policy requires broad interest in issues at the interface of science and society, and an ability to talk...in the legal, ethical and policy issues at the interface of biology and society.     Additional resources...

...target cell line, researchers often find themselves faced with a roadblock. In this post, we will provide ...concentrated by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin...starting. We recommend calculating the ratio of the surface area of the larger vessel to the 10 cm2 dish and...some final adjustments, this is generally a good place to start. If you don’t anticipate that you will ...relatively inexpensive and does not require an ultracentrifuge, PEG will precipitate all proteins in a solution...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

... by ultracentrifugation (75,000 - 225,000 g for 1.5 – 4 hours at 4 °C). After ultracentrifugation, virus...vectors) you can concentrate your virus by ultracentrifugation of the viral stock, followed by resuspension...packaging cell debris before proceeding to ultracentrifugation to avoid contaminating your transduction ... to concentrate a virus solution without ultracentrifugation, you can reduce the volume of cell culture...exchanging the medium after transfecting cells, replace the medium with half the volume typically used ...

... Specially constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous...targeting takes advantage of this natural process to replace a targeted genetic locus with homologous sequence...gene will be removed from the chromosome and and replaced by the resistance gene.The gene is thus disrupted...recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted gene is disrupted ...normally because one of its exons has simply been replaced with the same sequence flanked by LoxP sites while... while the resistance cassette has been placed into an intron.    Figure 4: A targeting vector...

...student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will...Clutter not only makes it difficult to clean your workspace properly, but can also disrupt the laminar air...materials into the hood. This will give you enough space to move around freely without causing spills. Also... than 80-85% confluent because cells like their space. If they reach confluence, they stop growing (contact...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology...

... they not only traced the 3-D path of the protein’s backbone in three-dimensional space, but their flexible...ribosomal subunit, he kept sliding his palm across the surface of the protein where the nascent polypeptide emerges...realized how perfectly flat the complex is on this surface”….where the complex is docked on the membrane of...serious way. We represented the Cas9 protein in a surface format made of rock-hard, inflexible plaster. As...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...

...in-house software has been crucial for us to keep pace with our current need to analyze nearly 300 sequences...sequencing data collection and analysis eventually outpaced our software, even with continuous updates. The...other bioinformatics purposes  Integrated user interface, so our QC team could initiate and monitor the...open-source sequence assembly tools at the appropriate place in our workflow without having to translate or convert...Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with the basic...smoothly operate the pipeline, we needed a user interface. In our next post, we’ll share our story about...

...of common genetically encoded neuronal tracers Neuronal tracers such as epitope tags and fluorescent proteins...Spaghetti Monster - A new and improved neuronal tracer With the limitations of epitope tags and fluorescent...fluorescent proteins, it has been crucial to develop new tracers that enable accurate labeling of neurons. As discussed...Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins such as GFP have been used...labeling experiment using smFPs and standard neuronal tracers. 2. Visualization of neuronal sub-cellular structures...

...simply embellish and adorn your lab space or your work from home space in a fun, festive holiday or winter...winter or holiday theme decor in your work from home space… but with a science twist. Maybe you have a collection...using life science catalog pages. Make your WFH space feel a little more festive this holiday season! ...Instagram as we share pictures of our decked out spaces.       ...

...(Brinkman et al., NAR). This technique uses the trace file generated in Sanger sequencing and a simple...Fig. 1: Overview of TIDE. The input is sequencing trace files of WT and edited cell lines. Then decomposition...and your Cas9-targeted cells. Simply upload both trace files to the TIDE tool online along with your sgRNA...outlined above), except that it uses a 3rd sequencing trace file from the donor DNA. This method can precisely...edited sequence would look like (the 3rd sequencing trace). While it requires a little extra wet lab work,...quantitative assessment of genome editing by sequence trace decomposition. (2014) Nucleic Acids Research Volume...

...non-scientists alike will march to advocate for science’s place in education, government, and civilization in general...attribute that enables science: “humility in the face of evidence” (4). Frustration among scientists has...aspects of research at National Aeronautics and Space Agency, the Environmental Protection Agency, and...inclusion of science) they come from many different places supporting many different positions. It would be...and 514 satellite marches are scheduled to take place around the world. There is no requirement that marchers...plan Science Magazine: NIH, DOE Office of Science face deep cuts in Trump’s first budget Environmental ...

...information is consolidated to one place, easily retrievable, searchable and traceable. However, switching from ...that bring more to the table in terms of data traceability and reproducibility of research. Electronic ... enables researchers to store everything in one place. From pictures to excel sheets, researchers today...enable researchers to keep all their data in one place and avoid scattering intellectual property across...run, ELNs enable labs to keep their data in one place and therefore make it easier for them to track and...

...versions obsolete, the techniques we use in lab are replaced by improved versions that save both time and money...observed. Today Southern blotting has been largely replaced by real-time PCR to answer the same experimental...unknown sequence. In the era before sequencing was commonplace, researchers used restriction mapping to investigate...this technology, radiolabelled nucleotides were replaced with fluorescently labeled ones allowing for fluorometric-based...discoveries (5, 8). While newer techniques have largely replaced these, these techniques still have useful applications...to recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly...