We narrowed to 174 results for: ADAP

1. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al.   You can also search by ...

2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...

3. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-...

4. A Deep Dive into BioBuilder

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   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current...

5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4...

6. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...

7. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...

8. Antibodies 101: Isotypes

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   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ... constant domains. This switching helps B cells adapt during the course of an immune response since it...

9. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ... in much of the world, we have found ourselves adapting to a new way of doing things. These new processes...

10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...

11. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ... Fig. 3 dTag mechanism of protein degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral...

12. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence...

13. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...

14. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

15. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...

16. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to work in your lab. Find...

17. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    Published

    July 18, 2019, 12:55 p.m.

    ...Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered in plants to conditionally...

18. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    Published

    July 11, 2019, 1:26 p.m.

    ...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

19. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...