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We narrowed to 173 results for: ADAP

Showing: 101 - 120 of 173 results

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Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

Published

Nov. 5, 2019, 1:28 p.m.

...years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research...
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

Published

June 6, 2019, 12:41 p.m.

...group inserted sgRNAs targeting various innate and adaptive immune genes into pXPR_053. They expressed this...
Hot Plasmids - February 2022

Type

Blog Post

Published

Feb. 22, 2022, 2:15 p.m.

...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...
Antibodies 101: Isotypes

Type

Blog Post

Published

Oct. 19, 2021, 1:15 p.m.

... constant domains. This switching helps B cells adapt during the course of an immune response since it...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

Published

Jan. 25, 2024, 2:15 p.m.

...Comparison of different Cas enzymes using CasPEDIA. Adapted from Adler et al. You can also search by ...
A Deep Dive into BioBuilder

Type

Blog Post

Published

Oct. 11, 2017, 1:44 p.m.

...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current...
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Type

Blog Post

Published

July 28, 2017, 1:30 p.m.

...cell differentiation (a cell type important for adaptive immune responses, which flies do not have!) (4...
Luminescent Imaging with Nano-lanterns

Type

Blog Post

Published

May 25, 2017, 2:30 p.m.

...Nano-lantern, showing the technique’s versatility and adaptability to many different contexts. Improving Nano-...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

Published

June 2, 2015, 6:06 p.m.

...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...
Plasmids 101: The protein expression toolbox

Type

Blog Post

Published

Feb. 16, 2023, 7:05 p.m.

... Fig. 3 dTag mechanism of protein degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

Published

March 19, 2019, 1:31 p.m.

...sequence TOM20 (pTriEX Tom20 mVenus BoPD). In dark adapted cells expressing these plasmids, tgRFP fluorescence...
Plasmids 101: Cre-lox

Type

Blog Post

Published

Jan. 13, 2015, 3:47 p.m.

...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

Published

June 14, 2018, 11:44 a.m.

...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Type

Blog Post

Published

May 15, 2018, 12:43 p.m.

...protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to work in your lab. Find...
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Type

Blog Post

Published

Aug. 20, 2019, 12:31 p.m.

...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...
Open Resources and Plasmid Tools For Studying C. elegans

Type

Blog Post

Published

July 18, 2019, 12:55 p.m.

...Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered in plants to conditionally...
Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

Published

July 11, 2019, 1:26 p.m.

...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Type

Blog Post

Published

April 6, 2021, 12:30 p.m.

...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

Published

Dec. 14, 2021, 2:15 p.m.

...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021. Let me leave...
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Type

Blog Post

Published

Aug. 24, 2021, 1:15 p.m.

...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

Showing: 101 - 120 of 173 results

Results per page:

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Hot Plasmids - February 2022

Antibodies 101: Isotypes

CasPEDIA: A Functional Classification of Cas Enzymes

A Deep Dive into BioBuilder

Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Luminescent Imaging with Nano-lanterns

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Plasmids 101: The protein expression toolbox

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Plasmids 101: Cre-lox

Adenoviral Vector Production and Troubleshooting

Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

Open Resources and Plasmid Tools For Studying C. elegans

Mouse Modeling, Part 1: Genetically Engineered Mice

Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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