Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 158 results for: Arch-GFP

Showing: 101 - 120 of 158 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...via phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly...around 100-fold. These improvements will allow researchers to better fine-tune the level of output gene ...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...be another method to assess transfection success. GFP is often used as a reporter and we will be covering...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for...and their applications can be exhausting. A quick search for "CRISPR", short-hand for Clustered Regularly...

  6. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...line, Hep3B. From left to right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay).   ...German Cancer Research Center (DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors...fluorescent proteins in their research. These awards help early career researchers attend a conference of their...opened up applications to research technicians and undergraduate researchers, in addition to postdocs ...postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where..., Pedre Pérez’s biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore.... Below, read about the five winners and their research.   Abhi Aggarwal: Glutamate sensing reporters...

  7. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...is a higher risk cargo for a researcher to come into contact with than GFP. Similarly, if the viral vector...than cell cultures do, and tired researchers are accident-prone researchers. Note that safety policies will...have become a regular part of basic biological research as well as clinical therapy. These biological ...in non-target cells, including yourself as the researcher.   Figure 1: Separation of retroviral ...need to be done in a biosafety cabinet. Many researchers choose to wear eye protection when they are working...likely to want to cut a few corners. For many researchers, viruses might be the most dangerous things they...

  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...targeting. By employing various AAV serotypes, researchers can precisely direct the calcium sensor to specific...complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino...

  9. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Expression with Cre-lox Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids...photoswitchable kinases in vivo with C. elegans. Researchers commonly use rectal bacterial infection to induce...

  12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...luminescence have been widely used in biological research for a long time, chromoproteins are just now being...history of fluorescent proteins in molecular biology research, chromoproteins have a shorter history. In 1987...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple... can be used, why not add some color into your research? If you have a favorite chromoprotein, either ...Addgene.org Find fluorescent proteins for your research Read our Molecular Biology Reference Find plasmids...

  13. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ..., Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral...fluorescent proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs... brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered...

  14. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...select both over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio... biological screens. For example, for cancer researchers, if growth properties in agarose are of interest...

  15. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...Biotechnology and Biological Sciences Research Council (BBSRC) and Medical Research Council (MRC) require authentic...the amount of money wasted in basic oncological research due to contamination are in the tens or hundreds...lines deposited with them and used by different researchers are free of contamination and match their original...perhaps a working cell bank has been made by a research institution (an excellent practice), or maybe ...Warwick University, gaining extensive cell culture research experience through working for a variety of pharmaceutical..., HeLa cells, and cell culture contamination." Archives of pathology & laboratory medicine 133.9 (2009...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...used this cassette to knock out genes, knock in a GFP cassette, and introduced site-directed mutations,...selection. In a subcutaneous cancer model, the researchers showed that after 24 hours, the unstabilized ...in nature, these systems have been adapted by researchers for many uses when plasmid retention without ...for long-term experiments. They’ve also helped researchers design and optimize cloning methodologies using...genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369. Kulakauskas....org Check out our molecular biology reference Search for a plasmid here Find plasmids from our special...

  17. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted... in Biomedical Research from Albert Einstein College of Medicine. Her doctoral research was conducted ...Figure 1). When behaving properly, FPs enable researchers to investigate the localization and dynamics ...co-mentorship Blossom Damania and Jack Griffith, her research focuses on the replication machinery of Kaposi...secretory pathway."Biochemical and biophysical research communications 430.3 (2013): 1114-1119. Pubmed...

  18. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...Fluorescent Protein Aggregation Catch Up On Your GFP Basics Get Advice on Choosing Your Fluorescent Protein...to monitoring localization; using FP timers, researchers can look at a single image of a cell and understand... production of the initial FP timer in 2000, researchers have realized how problematic aggregation-prone...expression. In a developing embryo, for example, a researcher could determine whether or not newly synthesized...synthetic biology, FP timers might be able to tell researchers whether old or new cells are better at producing...

  19. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial ... doors for scientific research - how will you use these systems in your research? Cary Valley contributed...developed and potential ways you can use it in your research. RNA editing advantages RNA editing has multiple... forward to the expanded use of RNA editing in research and its potential therapeutic uses We are also...

  20. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your Research     ...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...
Showing: 101 - 120 of 158 results