We narrowed to 458 results for: BLI
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TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m....Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time...2):131-3. PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM...nmeth.1664. PubMed. Find plasmids from this publication at Addgene. Addgene’s Fluorescent Protein...
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CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....Base editing publication highlights In the table below, you'll find a list of publications we've highlighted...editor was named ABEmax. In 2020, two papers were published describing additional ABEs evolved from ABE7.10...highlighted in this article. Publication Plasmids Highlights Komor et al., 2016 BE1, BE2, BE3 BE3... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog PostPublishedJune 11, 2019, 1:16 p.m.... have access to through your university or your public library. If you want to use pre-drawn images in...without the watermark, which are better suited for publications, if you sign up for a paid account. I’m proud...easier to give attribution, than for journal publications. Google Image Search Google image search is ... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....c)(3). That means we were formed to benefit the public, not private interests. On this nonprofit awareness...initiatives as opposed to fundraising. Aside from enabling us to make all decisions with the goal of benefiting... well. We work every day to do a better job of enabling more scientists in labs all over the world to ... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....interested public.” Subscribers to Scientific America would be considered the interested public and it would...podcasts A series of Tweets breaking down a new publication that could advance treatments for a rare disease... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....arrange permits to visit places not accessible to the public and retrofitting our car with a freezer wired to...nts, and is thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS...conditions. One example application from the publication is the removal of unwanted cDNA derived from ... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....science is much like industry but the “product” is publications—the pressure to produce is the same You get ... like you What is different about academia? Publishing in peer reviewed journals is absolutely necessary...is different about non-academia? Scientific publications are a nice to have, not a need to have Must ... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo ...PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al. “DNA-... PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog PostPublishedJuly 7, 2015, 3:36 p.m.... of print] PubMed. Find plasmids from this publication at Addgene. Other strategies for CRISPR/Cas9...2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and versatile genome editing...2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional Resources on the Addgene... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....she would be looking at where I had published, who I had published with and whether I would be able to...blogger! Seán was a PhD student at University College Dublin & a post-doc a Cambridge University where he studied... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....pathology, and by disabling neurons you can figure out what they are necessary for. We published our first paper...using the power of light. In a recent article published in Nature Methods, Boyden and his team describe... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m.... and written communication skills are key to publication and acquisition of funding. If a grant committee...Understanding) Writing Communication Publicity Consulting internships Communication...Blogging Writing Communication Networking Publicity Business competitions Leadership ... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence...animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a...rectify this the Shu lab first improved the self-assembling capability of sfCherry approximately 20 fold ... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...collaborative writing and this recent article published in PLOS Computational Biology (Frassl et al., ...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....funded by the public and performed by researchers who received their educations from public schools and ... BSc in Anatomy, the University of Otago had established the Centre for Science communication and I was... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....directly in DNA assembly will still yield scarless assemblies. Overview of NEBuilder HiFi DNA assembly method...simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the desired mutations... of these techniques require more complex DNA assemblies and improvements in these methodologies offer... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....libraries is well suited to large-scale de novo assemblies of short constructs like plasmids. The plexWell..., this has resulted in high quality NGS-based assemblies for over 3000 plasmids in the Addgene collection...of Toronto. He is an author of 20+ scientific publications and patents. ... -
Fluorescent Proteins 101: Luciferase
TypeBlog PostPublishedJune 24, 2014, 3:59 p.m....Imaging Improving Well-established High-throughput assays Improving Well-established Biological sources...describing the cloning of firefly luciferase was published in 1985, several studies utilized luciferase as... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m.... the example of our Antibody Guide, which was published alongside our new antibody services in the spring...through an editing process and final check before publication. Do research! Nearly everything we write entails...final review to catch those pesky typos, and hit publish. Making content is an iterative process. We are... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m.... now. Enter the SAM pooled library In a paper published in Nature in December, Konermann and her colleagues...Zhang lab for GeCKO, a lentiviral CRISPR library enabling genome-scale, knockout screening). As the Zhang...pooled CRISPR libraries, also available at Addgene, enabling systematic investigation of the cellular consequences...