Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 271 results for: CAD

Showing: 101 - 120 of 271 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...Activation of membrane receptors and signaling cascades conceivably allows the genome to sense the impact...different tissues in normal and disease states, their circadian regulation, as well as in important physiological...

  2. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular “spring...fluorescent proteins." Proceedings of the National Academy of Sciences 96.20 (1999): 11241-11246. PubMed PMID...

  4. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ... that there is a more emphasis today about non-academia and industry jobs. Since there are a variety of...and collaborative.” She knew she wanted to leave academia, and once she joined an investment club and discovered...

  5. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...In the last decade, the use of degron tags has become increasingly popular for the modulation of endogenously...degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073...

  6. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...’s program, the answer might be to continue in academia and pursue a PhD. However, if you’re anything ...process. COVID-19 has changed many aspects of academia, and the PhD application process is certainly ...

  7. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...within and outside of your organization. In my non-academic roles, some examples of negotiation have been:...anywhere in the world. Whether you are serving on academic committees as a tenured professor or signing contracts...

  8. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...PhD programs, mine was geared toward careers in academia or industry. Luckily, I discovered that there ...emphasize the important roles of scientists outside of academia One recent example: Don't call me a dropout: ...

  10. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...started my Twitter account recently inspired by an academic in literature who gave a talk to the women’s research...yourself as a real person, so that students see academics as well-rounded human beings. It’s also a great...

  11. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...of the National Academy of Sciences of the United States of America. National Academy of Sciences, 112...

  12. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ... the nucleic acid. Proceedings of the National Academy of Sciences of the United States of America, 54...genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556–1560. PubMed PMID: 2304918...

  13. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...Pubmed search for “AAV” and “brain” over the past decade gives over 22,000 results - an enormous amount ...L., Muñoz, K. A., Hsu, R., Kalwani, L., Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A....

  14. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...sequencing (“Third-generation sequencing”)  In the past decade or so, the newest kid on the block in the sequencing...chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74(12), 5463–5467. https://doi.org...

  15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...Ligand-Regulated Site-Specific Recombination.” Proc. Natl. Acad. Sci. USA 92(June): 5940–44. PubMed PMID: 7597057...Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl Acad Sci U S A 92(15): 6991–95. PubMed PMID: 7624356....

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983): 4784-4788. PubMed PMID...bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996): 6059-6063. PubMed PMID...

  18. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...problems surrounding cell culture over the last few decades some pharmacologists have tended to avoid cell ...comparable studies to be performed on a cell line even decades after it is initially isolated. Regrettably this...

  19. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...spectroscopy and microscopy." Proceedings of the National Academy of Sciences 114.23 (2017): 6005-6009. PubMed PMID...luminescence imaging." Proceedings of the National Academy of Sciences (2015): 201418468. PubMed PMID: 25831507...

  20. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...mediated labeling." Proceedings of the National Academy of Sciences 106.35 (2009): 14896-14901. PubMed ...Caenorhabditis elegans." Proceedings of the National Academy of Sciences 113.8 (2016): E1074-E1081. PubMed PMID...
Showing: 101 - 120 of 271 results