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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7 results for: CAD

Showing: 1 - 7 of 7 results

  1. CRISPR Guide

    Type
    Guide
    ...enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is ...separate Cas enzymes that form a complex called Cascade (see Cas3 for more details), while type V-K systems... by Cas9 systems. Cas3 must be paired with the Cascade ( C RISPR- as sociated c omplex for a ntiviral ...de fense) complex to initiate DNA degradation. Cascade is made of five proteins: Cas8 (Cse1), Cas11 (Cse2...Overview of editing with Cas3 Browse Plasmids: Cascade-Cas3 Want more information on the wide variety ...nucleases like Cas9 and Cas12a (also known as Cpf1) Cascade C RISPR- as sociated c omplex for a ntiviral de...Lewis, J. D., & Bondy-Denomy, J. (2020). A compact Cascade–Cas3 system for targeted genome engineering. Nature...

  2. Chemogenetics Guide

    Type
    Guide
    ...potently activated by an inert ligand. Proc Natl Acad Sci USA. 104:5163–68. PMID: 17360345 (Link opens...biological light sources for opsin activation. Proc Natl Acad Sci USA. 113:E358-67. PMID: 26733686 (Link opens...specifically designed Gi-coupled receptor. Proc Natl Acad Sci USA. 95:352–57. PMID: 9419379 (Link opens in...regulation of β cell function in vivo. Proc Natl Acad Sci USA. 106:19197–202. PMID: 19858481 (Link opens...

  3. Adenovirus Guide

    Type
    Guide
    ... referred to as "gutless" or high capacity AdV (HCAdV) vectors. Here, all viral sequences are deleted,...accommodating ~36 kb of genetic cargo. Production of HCAdV vectors requires using an additional adenoviral ... adenoviral genes. The biggest challenge of the HCAdV vector production is to ensure no carryover from...recombinant adenoviruses . Proceedings of the National Academy of Sciences of the United States of America, 95...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...been widely used in the research community for decades. Wildtype gamma-retroviruses have been engineered...-expressing cells. Proceedings of the National Academy of Sciences , 94 (16), 8640–8645. https://doi.org...

  5. Modular Cloning Guide

    Type
    Guide
    ...Plasmids for reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA...

  6. Plan Your Experiment

    Type
    Guide
    ...manipulate the genome with relative ease. Over the past decade, researchers have expanded the CRISPR toolbox to...

  7. Lentiviral Vector Guide

    Type
    Guide
    ...been widely used in the research community for decades. Wild-type lentiviruses have been engineered into...
Showing: 1 - 7 of 7 results