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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 659 results for: Clu

Showing: 101 - 120 of 659 results

  1. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    .... A quick search for "CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats...few references for the double nickase system, including one from the Zhang group. Q2: Should I add the...off-target effects depends on a number of factors, including: how closely homologous the off-target sites are.... Thank you, Le! This blog post was updated to include all FAQs from Le Cong on June 24, 2025 by Alyssa...of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson Point mutations....

  2. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    .... In the case of stem cells, these factors may include cell type, the disease being treated, and other...immunological, and environmental factors. How to include sex in experimental design Recognizing the importance...Institutes of Health (NIH) now requires that sex be included as a biological variable in all NIH-funded research...differences in effects on the sexes come from, one clue or potential place to start research is by considering...

  3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...also be designed with other DNA-binding domains, including the commonly used LexA and rTetR systems such ...could be delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord....easily adaptable to neuroscience applications, including optogenetics, and they should make it much easier...Other proteins may also be utliized as scaffolds, including the set of red fluorescent proteins. The T-DOGGs...

  4. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...fat-soluble hormones, vitamins, and dietary lipids. Included in this family are receptors for endocrine steroids... to treat a multitude of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular...these key regulatory molecules, and has provided clues to their previously uncharacterized cellular roles...interface, expanded numbers of curated molecules, including 400 or so coregulators, and a greatly expanded...

  5. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...contains both fragments of split mVenus (aa 155), includes I152L in N-terminal fragment An enhanced recombinant... Dan Mulvihill mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment Constant rate of ...  When designing experiments, it’s important to include proper controls to assess the behavior of the split...Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    .... This tool-kit includes an array of FPs as well as additional useful tags, including 3xFLAG epitopes,... chromatin. A fluorescent protein can still be included for visualization purposes, but the key component...green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte, and...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ... delivery technologies have been investigated including lentiviruses, adenovirus, and inorganic delivery...additional safety benefits: they lack all viral genes, including those that are responsible for integration into...capable of infecting a wide range of cell types including but not limited to muscle, liver, and brain cells...clinical trials have been directed at diseases including but not limited to hemophilia B, LPL deficiency...

  8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...for affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman...implementation and compliance for the FLSA changes, including finding money in already tight lab budgets and...their advocacy skills. Friday’s symposium will include a discussion on effective advocacy strategies on... on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington ...

  9. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...unstained flow controls). Spectral flow cytometers include a tool called “autofluorescence extraction” which...various tissue types varies, it is critical to include an unstained sample for each tissue type you are... the expression levels of the markers you are including in your panel. Highly expressed markers should...during unmixing. Additionally, it is critical to include unstained beads or cells within your reference ...

  10. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...Over the years, Addgene’s repository has grown to include many CRISPR tools.  Distributing and depositing...this tell us? Well, it’s hard to draw any exact conclusions, but it certainly suggests that Addgene depositors...approximately 104 plasmids shipped per working day, including appropriate MTAs and customs forms. Of course,...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story that...

  11. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth repeat Nobel Prize winner...proposed as a mechanism for increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014;...promote professional success (many of my students include their in-class research experience on their resumes...experiences can make scientific research more inclusive. CBE Life Sciences Education. 13(4):602-606. Brian...

  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...proteins have replicated many of the properties once exclusive to small-molecules: red-shifted spectra, ion sensitivity... have been developed based off of this concept include: Self-labeling tags (e.g., SNAP-tag, HaloTag, ...innovative labeling strategies, several groups—including mine—have been revisiting the old chemistry of...and contain polar groups (Figure 2b), which can preclude their use inside living cells. We recently discovered...

  13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...beer. Ha! We had over 60 people join the fun - including a lot of future Addgenies as shown in the photo...employees. We now have a team of three (myself included) that plan big company events and fun things to...people you spend 40+ hours with each week. In conclusion - get out there any have some fun together! Laura...at all really! We've had plenty of events that included a simple corn hole set and a cooler. Chances are...

  14. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...episodic memory, a special type of memory which includes autobiographical events (see Jin & Maren, 2015...visualization of the morphology of individual neurons, including dendritic and axonal extensions and, most importantly...some engineering, RABV and HSV, but also others including pseudorabies virus type 1, vesicular stomatitis... Addgene's AAV guide)  Commonly used serotypes include AAV1 – 9, with most of these serotypes capable ...future developments in the field of AAV vectors. Conclusion An ideal comprehensive toolset for neuronal tracing...

  15. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...an interview next week and it includes a chalk talk. What should I include in my chalk talk?" So what exactly...proposed approach to resolve the knowledge gap? This includes the overview and the individual aims. Describe...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...

  16. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...completely understood, but some possible reasons include: Metabolic pressures - it takes cellular resources...when designing a full gene. A non-exhaustive list includes: Codon abundance relative to cognate tRNA abundance...insoluble, nonfunctional globs of protein known as inclusion bodies that will generally segregate with the ...assay to make sure your protein isn’t forming inclusion bodies and is folding properly. References 1...

  17. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...the CRISPR workflow. For a multiplex sample, ICE includes visual representations of all detected edit types...indel.   For a complete guide to ICE analysis, including detailed explanation and examples, read the paper...of the DNA-targeting region of the guide RNA, excluding the PAM sequence. PAM Sequence - The Protospacer...stack Genome Engineering Solutions, which also includes our CRISPR knockout guide design tool, guide validation...

  18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... flexibility is their broad range of targets, including proteins, peptides, amino acids, drugs, metal ...biosensors are used in a variety of applications, including detection of environmental pollutants, drug discovery...aptamers for use in many entirely new applications including aptamer based fluorescent biosensors. Aptabeacons...-based instead of protein-based fluorophores, including: Environments with higher temperatures, since...

  19. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...you start reading, there will be a temptation to include every piece of information that was ever published...figures/schematics or tables. And don’t forget to include citations so that people can go back and read the...studies that reported mutations in that protein (including how frequently a mutation was found and the size...doesn’t have to be revolutionary, but you could include a few comments on where you think the field is ...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~3.3 kb) from Streptococcus... approximately 1350 amino acids in size, which includes SpCas9, and shortern orthologs approximately 1000...in mouse liver.  Two other smaller Cas proteins include Cas12b and CasX. While there are no papers yet ...capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation. Split...
Showing: 101 - 120 of 659 results