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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 364 results for: DUR

Showing: 101 - 120 of 364 results

  1. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...different CellTag libraries at multiple timepoints during reprogramming. These library-specific identifiers...different CellTag libraries at three time points during the 28 day study: prior to reprogramming (CellTagMEF...

  3. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...mentor or anyone else available to directly help you during your visit. Reach out to your bench mentor (if ...and rights for anything that could be generated during the visit. If you don’t feel comfortable initiating...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...translation is key to cellular function, especially during development or stress. With ribosome profiling,...double labeled, indicating translational repression. During later stages, single RFP-labeled osk and Oskar ...

  6. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When... He first heard about RNAi at his MIT interview during a lecture given by Phil Sharp. After a few rotations...

  7. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...job. How can you incorporate the skills developed during graduate school into your resume? How can you stand...transferable skills? See Developing Transferable Skills During Science Training and So You Think You have Skills...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a form of oxidation (...physiologically relevant exposure in intensity and/or duration. Both of these strategies carry the risk of off-target... contain the ccdB cassette, which is not copied during the PCR amplification step, so that only vectors...

  10. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ... club of its kind, running from start to finish during the academic year and remaining affordable for ...BioBuilder textbook, a laboratory kit, and support during the academic year from remote scientist mentors...

  12. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...mismatched basepairs or small indels that occur during DNA synthesis, and protomoting or repressing protein...DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired mutation...

  14. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case...

  15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...general areas of interest and what you want to cover during the year. Suggest that each person bring 3 topics...preparation for the meeting or to be read or watched during the meeting.  Scientists do better with process...

  16. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...stranded structure produced is known as an R-loop. During transcription, RNA is synthesized from a DNA template...DNA hybrids Persistent RNA:DNA hybrids that arise during transcription are typically recognized by RNase...

  17. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...polyclonal antibodies include any antibodies produced during the immune response.   Polyclonal antibodies...If your protein might become slightly denatured during your experimental protocol, using a polyclonal ...

  18. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic protein...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...

  19. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    .... Some microenvironment cells improve tumor’s durability, while others repress it. Unfortunately, as a... a whole, microenvironments promote tumor durability instead of repressing them, making it even harder...

  20. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ..., antibiotic resistance, restriction sites used during cloning, and most importantly, the predicted full...ensuring quality, as it will catch any errors made during the cloning, sample handling, or sequence assembly...
Showing: 101 - 120 of 364 results