...increase plasmid yield. Keep in mind that you might need to use multiple techniques for a particularly stubborn...bacterial strain to see if additional steps are needed to optimize yield. You might want to include an... high levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein...optimize your growth time, temperature, and shaking speed to maximize cell density and plasmid yield as these...hours, but cultures with lower copy plasmids may need to be grown for 20 hours or more to achieve maximum...culture volume and you should ensure the shaker speed is fast enough for sufficient gas exchange in the...those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn’t work...

...What is a transferable skill? Time management. I needed it when balancing a handful of demanding courses...work, bills, (at times) a social life and rest. I need it just as much in my current role as Customer Support...to do the job. You may think this means you’re guaranteed to get the job, however, many times the hiring...your boss approaches your team with a project she needs one of you to lead. At first you might think, “I...change happens at your organization, when your boss needs someone to step up, when a new venture presents ... Say your manager approaches you with glowing feedback from a client you helped. Maybe you awkwardly ...other things you have to do. Pause! Enjoy that feedback, bask in the glow of a job well done, take some...

...get this far and realize you need to apply different filters you do not need to start a new search. Simply...can narrow your search results to exactly what you need: Better searching with filters Not every plasmid...filters lets you drill down to the exact plasmid you need. The ability to apply a wide range of filters isn... your search results to match your experimental needs. Remember, only filters with results will show up...Serotype: Choose the AAV capsid or lentiviral prep needed Tags: Identify a specific marker protein Use: Similar...website and we hope it leads you to exactly what you need with every use. If it doesn’t and you find yourself...

... thank Seeding Labs for this continued opportunity to get plasmids to the researchers who need them the...Sciences in Ghana will be receiving the third Addgene/Seeding Labs plasmid grant and plans to use the grant to...Future awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of applicants... seeding labs... seeding labs plasmid grant...

...review. If it’s a well-researched field, you may need to get specific to make sure your article doesn’...reviews to get a list of research papers that I needed to read. Once I had an idea of the themes in the...word of advice: when reading, don’t feel like you need to read every paper from start to finish. Find the... comment about writing: initially I felt that I needed a huge chunk of time to sit down and get anything...telling the story. Like any editing process, you will need time away from the article to be able to keep editing...normal and it means you’re on the right track!  Get feedback After you’re done editing, send it to some actual...actual experts in the field for their feedback on the scope and the content. Submit your article! Overall...

...understand the principles of gating, you’ll first need to learn a little bit of theoretical background ...software will allow you to adjust this setting as needed. To figure out the appropriate voltages to observe... through maximum current (H), the time the cell needed to pass through the laser (W) and the area under... current and thereby the signal strength. So, we need to adjust the voltage in the cytometer’s software...interest). Let’s double-click inside our gate and proceed to the next plot! Pro tip! If you only get very...gated on your cells of interest, you will likely need to exclude all duplicate events (two cells stuck...analyzed on their staining signals. For this, you’ll need to gate based on the proportion between H and A ...

..., making it easier to produce the constructs you need. Expression vectors within this kit contain a variety...novel and powerful pyranopyridine derivatives." Proceedings of the National Academy of Sciences 113.13 (2016...terminators in this series are just the tool you need. The plasmids in this portion of the BIOFAB Plasmid...-time observation of metabolite production." Proceedings of the National Academy of Sciences 113.9 (2016...pastoris depending upon your specific protein folding needs. The pCri system has recently been used to study...adaptive evolution of antibiotic resistance." Proceedings of the National Academy of Sciences 109.52 (2012...regulation using the PUF protein family scaffold." Proceedings of the National Academy of Sciences 108.38 (2011...

...correction (about 4%) is needed to see muscle improvement, with only 30% correction needed for normal function...multiple requirements must be met. First, CRISPR would need to be delivered to both cardiac and skeletal muscle... editing occur, the CRISPR components would only need to be expressed for a short period of time, which... mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining (NHEJ) ...therapy closer to the clinic, much work is still needed. First, CRISPR delivery must be optimized to: 1...obstacles remain for CRISPR gene editing, but given the speed at which the technology is advancing, precision ...

...redesigned our website to make it easier to find what you need and to bring it in line with current Web Content...global scientific community. As we enter 2025, your feedback continues to guide our innovation.  In response...search experience so you can find exactly what you need, faster. We’re also building a portal to easily ...landscape is evolving rapidly, and with it, the needs of researchers worldwide. Through our growing community...we're equipping every scientist with the tools they need to push the boundaries of discovery. As I look ahead... E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F., Bradbury, A. R. M., Cable...

... whose infection has failed all #antibiotics. We need lytic non-lysogenic phage URGENTLY to find suitable...help clinicians and scientists quickly find much-needed phages for patients.   A phage directory is crucial...chance that an early request for phage may not be needed if other antibiotics work. “We don’t want to cry...phage community until we have someone that really needs it, but then it might to be too late,” Sacher says...treatment occurs. In addition to providing much needed information and potential for successful phage ...phage therapy, Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires...

...waiting multiple days for a reply to an email. You need answers: the right ones, quickly, and this is why... to make our support team available whenever you need them, but sometimes that’s not enough. With your...and make a phone call or write out an email. You need help right at the moment you’re looking for it, ...Center to make sure that, whatever your support needs are, we’ve got you covered! In addition to the Help...making it easier than ever to get the support you need. If for some reason you can’t find the answer that... the support we provide based on your valuable feedback. This is Addgene after all, and making your research...

...: Validation post. The primary antibody control needs only to be run once for each primary antibody that...that will be used (Burry 2011). It does not need to be repeated for each experimental run as long as the...addition to the above-mentioned safeguards, you’ll need to test the secondary antibody in the absence of...detected, the level of noise will determine whether it needs to be addressed. In order to correctly interpret...interpret your results, the signal from your target needs to be significantly higher than the background noise...antibodies in the stain. In situations like this, you may need to find alternative primary or secondary antibodies...samples in different lanes in a western blot, you’ll need to include a loading control. A loading control ...

...them You don’t need to explain as much of your science in a weekly lab meeting as you’ll need to for an outsider...telling it. End at the end A story needs a good start point and it needs a good end point. One thematically... a Good Story Start at the start Any good story needs a solid point to kick off at. Whatever you’re working... outsider. And you won’t need to dive as deep into your research at an outreach event as you might in ...

...audience can relate to. It doesn’t need to be anything fancy; it just needs to capture your audience’s attention...competing desires or (3) the presence of obstacles that need to be overcome. Conflict, and its inherent tension...(nonfiction) story. For there to be a story, you need to have conflict. There are a lot of ways to introduce...the Conflict  Once you have your conflict, you’ll need two other things to complete your story: an introduction...listen or watch) further.  Second, the introduction needs to provide enough information so that the audience...

...101: Control Plasmids Need help thinking about the types of controls you’ll need for your next experiment...posts aim to give researchers the background they need to get started using plasmids and the revolutionary...Scientists: Why and Where Do I Find One? If you need help making a transition into a new job or maybe... CRISPR/Cas9 to make a change to the genome, you need to check whether you’ve made the right one. In this...us through some common controls and why you might need them. Happy Reading!   Resources on the Addgene...

...quickly learned that my approach needed to adapt based on individual needs. For instance, I had one colleague...shift and navigate the pitfalls? Here's what you need to know.   Figure 1: Management can seem ...steps to a lab assistant. However, I recognized the need to shift my mindset to empower my team and allow...highly self-motivated and excelled when given the freedom to explore projects autonomously. Failing to recognize...managers fail to set expectations or provide timely feedback, leading to unproductive dynamics. I experienced...

...example, I used to constantly underestimate time needed to prep for an experiment (labeling tubes never...after running a protocol a few times, you no longer need the full checklist. Great! But don’t just stop using...into one. Soon you’ll figure out what prompts you need to consistently and accurately run a protocol.   ...they use them.    Prevent Interruptions If you need to focus, a large set of headphones can act as a...wall of pipette tip boxes when she particularly needed to focus, which was extremely effective. Others...at the bench. Don't be afraid to ask for what you need to be successful! Slowing Down At the suggestion...

...system, but the available reagents do not match your needs. If YFG is thrillingly new, you might have to make... germline. To begin this process, you will first need to do some molecular cloning. Depending on whether...trying to overexpress or knockdown a gene, you might need different plasmids. Harvard’s DRSC/TRiP Functional...different fly lines. Also, if there is a chance you need to combine multiple transgenes in one fly, having...express multiple transgenes, remember that you’ll need to integrate them all at different loci. Fortunately...for use? Random insertions take longer because you need to determine in which chromosome your transgene ...

...means the mentee needs to be open to change and constructive suggestions and feedback. The best thing ... able to: Help you identify specific skills you need for your current position Understand your interpersonal...of the people you work with Give more informed feedback (for example, they may be able to hear you present...elsewhere is able to: Help identify the skills you will need to make a change Provide perspective on core values...at another company/department Give more candid feedback and be able to discuss more confidential topics...

... have its limitations or that other technologies need to be discarded but clearly we are in an era of ...focuses primarily on behavior, you don’t necessarily need to use electrophysiology to prove that your optogenetic...required. In any given optogenetics experiment you may need to inject virus into 20 or more mice and the injections... die injection prior to using the virus. You may need to adjust coordinates based on your own method of... limited by the mouse line itself. Generation, breeding, and maintenance of a mouse line is also an expensive...terminal also affects the length of time you may need to allow the virus to express. For example, if you...surgeries and viral expression, you are ready to proceed to the material science part of optogenetics. In...