We narrowed to 119 results for: Endog

1. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system." Proceedings of the National...

2. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ...light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11:198–200 . https...

3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

   Type

   Blog Post

   Published

   Oct. 27, 2015, 3 p.m.

   ...gram negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using...

4. Plasmids 101: Control Plasmids

   Type

   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...produce a similar product, but do not target an endogenous gene in your experimental cells. For example,...

5. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...requiring anaerobic conditions, proteins that bind endogenous fluorescent co-factors can be used. Examples ...

6. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...CRISPR/Cas mediated HDR) enables the tagging of endogenous genes close to native expression levels. However...

7. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

   Type

   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ...XFP can be used to amplify the signal. Imaging endogenous fluorescence in live animals only permits visualization...

8. CRISPR 101: Validating Your Genome Edit

   Type

   Blog Post

   Published

   Nov. 3, 2022, 12:15 p.m.

   ...this method, but your edit doesn’t affect any endogenous restriction enzyme sites? Never fear! You can...

9. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   Published

   Oct. 5, 2021, 1:15 p.m.

   ...Table 1) were optimized for efficient binding of endogenous BV.  When imaging NIR FPs, consider using light...

10. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...Astrocyte-selective AAV gene therapy through the endogenous GFAP promoter results in robust transduction ...

11. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...demonstrate that the antibody efficiently binds to endogenously expressed protein at physiologically relevant...

12. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ... promising strategy for linking RSM inputs to endogenous gene expression (2). Our paper (1) explains ...

13. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...genome following donor removal, which alters the endogenous DNA sequence and has been suggested to cause ...

14. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ...monomeric state is less likely to interfere with endogenous protein structure and function. By generating...

15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...2009). However, these reagents can bind numerous endogenous biomolecules in cells nonspecifically, and they...

16. CRISPR 101: Cytosine Transversion Editors

    Type

    Blog Post

    Published

    March 25, 2025, 1:15 p.m.

    .... They conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition...

17. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

18. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...into TZAP function, Li and colleagues observed endogenous TZAP localization at telomeres and found that...

19. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ...Sequestration is accomplished by knocking down your endogenous protein of interest and co-expressing its replacement...