Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 182 results for: GRN

Showing: 101 - 120 of 182 results

  1. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...target DNA sequence as directed by the guide RNA (gRNA), while the CRY2PHR-activator fusion floats freely...occurs. Bubeck et al. expressed a telomere-targeting gRNA and a dCas9 fusion protein with three red fluorescent...Photocleavable ssDNA oligonucleotides Cas9, photocaged-gRNA Gene editing Manna et al., 2019   NPPOC-caged ..., 2020   NPOM-caged nucleobases Cas9, photocaged-gRNA Gene editing For more details, check out this...

  2. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity to a 20bp target sequence...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that ...modules are comparatively small allowing multiple sgRNA constructs to be introduced simultaneously in a ...

  3. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...re-amplified our distribution stock of the Brunello Human gRNA library, and we thought it would be a good time ... the plasmids are all very similar sizes, like a gRNA library, this is less of a concern, but Addgene ...confirmed using next-generation sequencing (NGS). For gRNA libraries, you’ll design primers to create 200-300...

  4. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...site that is not a perfect match to the guide RNA (gRNA) used to target the genomic locus of interest. As...of Sniper-Cas9 to different Cas9 variants using gRNAs of different lengths (Lee et al., 2018) and found...and sensitivity across a large number of loci and gRNA lengths. Recent alternatives to Cas9 While Cas9 ...

  5. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ... a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an...it won’t tell you whether the vector contains the gRNA insert or not. A blue-white screen, however, can...and distinguish between plasmids that contain the gRNA (white) from those that do not (blue). Let’s take...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library can ...interactions between paralogs. The exon-deletion hybrid gRNA library deletes human alternative cassette exons...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in human cell...limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12, which...Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation...

  8. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...treated with a folA gRNA were more sensitive to trimethoprim when compared to control gRNA treated or untreated...promoters, antibiotic resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...Then choose which Cas enzyme to use and design a gRNA to where you would like to introduce the edit. Finally... (Yu et al., Nat Chem Biol). Mutate the PAM or sgRNA sequences Cas9 likely goes through multiple rounds....org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out our CRISPR Guide...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single gRNA expression. Although....org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check Out Our CRISPR Guide...

  11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... E and the N2 gene of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences, Cas12a activity... Cas13, upon finding complementarity between the gRNA and a given fluorescently labelled substrate, gets...Fluorescently labelled dCas9 complexes containing sgRNA that have no mismatches bind to the immobilised ...

  12. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...that Cas enzymes can continue to cleave DNA if the gRNA/PAM site remains intact. In other words, Cas9 will...resulting mutations after an HDR event, ensure the gRNA or PAM site is disrupted in the donor. Choosing...Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out our CRISPR Guide...

  13. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...many software tools predict off-target effects of sgRNAs using computational methods. They identify possible...mismatches based on the sequences of the genome and sgRNA. This is a good starting point for most researchers...position of the mismatch along the length of the gRNA given the stricter sequence requirement at the terminal...

  14. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...Addgene gave to me: tips and tricks for designing gRNAs for CRISPR editing. On the fourth day of CRISPR,... day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR blog series...

  16. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...stem cells with a lentiviral vector containing a gRNA. These transduced stem cells can be implanted in...: Validating Your Genome Edit How to Design Your gRNA for CRISPR Genome Editing CRISPR Cheat Sheat Resources...

  17. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, developed by Yiping Qi's...Shirley Liu's lab targets all mouse genes with 10 gRNAs per gene. The guides are optimized to maximize on-target...

  18. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...system identifies and binds a genomic target using a gRNA, and an affiliated TniQ protein recruits transposition...integrate large sequences into genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. ...

  19. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...including the cell line/tissue type, enzyme system, sgRNA library and other details relevant to screen result...gene-level scores provided by authors rather than sgRNA-level sequence read scores. The original supplemental...
Showing: 101 - 120 of 182 results