We narrowed to 169 results for: Library
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TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....version,” she said. “If we learn how to make the library even better, we would be very happy to share updated...
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Save Time and Money by Making Your Own Competent Cells
TypeBlog PostPublishedJan. 28, 2014, 3:10 p.m....transformation (for example, if you are working with a library or an especially low concentration of plasmid),... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m.... Your experiment involves making a large sgRNA library to thousands of genes and targeting and sequencing...clonal differences. If you are performing CRISPR library screens, the same principle holds true - the higher... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....retroviral vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1...propagation of large plasmids, ligated DNA, and libraries, nalidixic acid resistant. endA1 glnV44 recA1... -
Your Top Requested Plasmid in 2016!
TypeBlog PostPublishedJan. 11, 2017, 8:47 p.m....editors, Casilio, CombiGEM, and a variety of pooled libraries. Not all of these technologies were without controversy... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m....genes were constructed as well. They called this library the CTD2 FASMIC Plasmid Collection for “Functional... -
Editor's Choice, September 2016
TypeBlog PostPublishedOct. 7, 2016, 4 p.m....a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....control process because of its expansive feature library and useful tools. In this blog, we’ll walk you ... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....several shout-outs for making popular screening libraries like Brie and Brunello available to the scientific... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....dinner which took place at the New York Public library. Being in the presence of scientific heroines is... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....towards using synthetic single-domain antibody libraries to generate new sdAbs. There has also been some... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m.... conditions when you’re building your antibody library! References and Resources More resources on Addgene... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like to knockout a gene, replace... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m.... fragments can then be purified for subsequent library preparation and high-throughput sequencing. Improvements... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog PostPublishedJune 11, 2019, 1:16 p.m....access to through your university or your public library. If you want to use pre-drawn images in your figures... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....Browse the CRISPR Collection Find CRISPR Pooled Libraries ... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m.... ligation for next generation sequencing (NGS) library construction, and circularization of dsDNA (6)....in applications such as routine cloning or DNA library preparation. However, when a method relies on accurate... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....as effective substrates for tagmentation-based library preparation procedures. This finding eliminates... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....browse/article/6441/ Resources: An open source library of characterized aptamers and supporting publication...