...enable the ability to study life’s innovations from anywhere in the tree of life.” Accepted proposals were...hydra and hydra have asexual and sexual reproductive life cycles. One can inject plasmid DNA in the eggs to...

...within the scientific community, as they allow for simplified transformation processes, and can be used by ...Gram-negative  Alcaligenes eutrophus, Bartonella bacilliformis, Bordetella spp., Brucella spp., Caulobacter...expression in bacteria. Methods in Molecular Biology (Clifton, N.J.), 765, 327–343. https://doi.org/10.1007/978...

...multiplex immunolabeling of brain. eLife, 8, e43322. doi: 10.7554/eLife.43322. PMID: 30667360....also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

...including the development of crops with longer shelf life and the development of disease-resistant crops to...expression include the ubiquitously expressing cauliflower mosaic virus 35S promoter (CaMV 35S) or ubiquitin...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...

...though they are aware that only 14.3% of PhDs in the life sciences end up in tenure track position 5 years...academic researchers themselves(44% of postdocs in the life sciences name faculty-research as their preferred... and enjoyable path for people from all walks of life will continue having conversations like those at...

...multiplex immunolabeling of brain. eLife, 8, e43322. https://doi.org/10.7554/eLife.43322 Brewer, M., McDonough...whichever targets you like. Plus, indirect IF amplifies the signal since multiple secondary antibodies...

...make a huge difference for your future career and life. You might see a lab head as choosing you, but in...I take to get to work?”; we often make important life decisions without much logic - even choosing a training...whom Expanding your skillset Scientists are lifelong learners. I believe we won’t be happy if we aren...

...register enables the design of primer pairs that PCR amplify in some states but not others. Because samples ...not be very informative – each primer pair will amplify even if its target states are only represented ... determine the fraction of registers that are amplified by a particular primer pair. Then, by leveraging...

.... Though I had spent the first few decades of my life following a very straightforward conveyor belt from...all the career spaces I explored for my post-PhD life, the science policy community is the one that was...these activities leading to a rich day-to-day work life.  Action Items:  Join the National Science Policy...

...expression profile at multiple steps from plasmid amplification in bacteria to transgene expression in vivo....are typically employed during viral plasmid amplification (Fischer, KB. 2019). This means that all DIO...of spontaneous reversion during AAV plasmid amplification. On the left homologous pairs of scrambled cre-recombinase...

...is an assistant professor at the University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies...choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers...is an assistant professor at the University of California, Santa Cruz where his group focuses on gene regulation...

...Stephanie Hays, a researcher at the University of California Berkeley. It’s been half a year since the march...rift where our integrity is questioned and cuts to life-saving research are being proposed.  It's our responsibility...studying an evolutionary arms race at University of California, Berkeley. She is a scientist with a passion ...

.../bd-spectrum-viewer.   But what is the real-life advantage of a brighter fluorophore? Strong brightness...en-us/resources/bd-spectrum-viewer.   To make life easier, always try to use fluorophores with distant...immunity. When he's not in the lab, Paul enjoys weightlifting, sailing, and reading.   More resources on ...

...reverts back to a pluripotent state, which then proliferates and is redifferentiated to a different cell ...stage (iPSC formation) [2, 3]. The iPSCs then proliferate and redifferentiate to another cell type of choice... More, due to selection from more cycles of proliferation Less Cell types All types Less Ease of genetic...

... by cells and to the uncoating step in the viral life cycle. Using AAV for neuronal tracing AAV vectors...plasmids at Addgene). The Gradinaru Lab at the California Institute of Technology generated novel AAVs ...Together with scientists at the University of California, Berkeley, the Janelia Research Campus also developed...

...to our latest podcast all about graduate student life and how establishing a thriving graduate student...Healthcare Consulting: A Door to the Business of Life Sciences 6 Tips for Grant Writing 5 Reasons to Use...

...and talk to us and wish them the best of luck in life beyond graduate school. Ben J. Vincent is a Ph.D...fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome biology and...

... Sponsorship from Addgene and Harvard Integrated Life Sciences (HILS) made it possible to offer DayCon...attendees and getting the chance to hear more about what life is like as a student researcher. The future of DayCon...

... of mutations they identified included gene amplifications and fusions, missense mutations, and indels...cell lines and any mutations that that confer proliferation advantages will be revealed in the absence of...

...employees created and designed for the Hall of Human Life at the MOS. (If you want a fun refresher on the ...of requesting and sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey...