We narrowed to 126 results for: NUA
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TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....in vivo.* Final thoughts The ease of use and continual improvement of fluorescent proteins makes them...
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CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as HIV. By design... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....next process. Similar to our first attempt, we manually inspected the output with a print statement at... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....Academy of Sciences. In the Yang lab, we’re continually optimizing the use of CRISPR editing systems ... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....gRNA) sequences. But these approaches rely on continual expression of both dCas9 and the gRNA. This expression... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....Hispanics and Native Americans in Science’s (SACNAS) annual conference all foster mentorship in different but... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....just 2 examples of organizations that hold large, annual conferences. Gordon conferences offer more focused... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....precise but not prolonged inhibition of CA1 neurons attenuated conditioning, which suggests that other regions... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m.... I needed to be close on a subject, or I could manually control the camera by hand and zoom from a distance... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m.... Mork, Jul 27, 2020; updated by Susanna Stroik January 24, 2023. DNA damage drives genome instability ... -
Exploring Applications of the Bioluminescent HiBiT Tag
TypeBlog PostPublishedOct. 2, 2025, 1:15 p.m....Tsien R. Y. (1998). The green fluorescent protein. Annual Review of Biochemistry, 67, 509–544. https://doi.org... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 2:15 p.m....expertise! This post was originally published in January 2025 and updated in August 2025. References and... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....published by Simpson, Sacher, and Szymanski in January 2016 in Viruses (5). Please refer to the paper ... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....undergoing RNAi through the fluorescent reporter and manually extracted. This process is tedious and time consuming... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....No single person could possibly understand every nuance of how kits work at Addgene, but with the help ... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....lasts five years. Most universities and institutes annually advertise for Group Leader positions starting ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog PostPublishedMarch 21, 2024, 1:15 p.m....acceptors in their product descriptions, or technical manuals. Specific applications and matching dye/ligand... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....excluding out of focus light. However, they also continually dose the sample from top to bottom with excitation... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....The development and application of optogenetics. Annual review of neuroscience. 2011;34:389-412. doi: 10.1146...