We narrowed to 332 results for: NUC

...improve nuclear localization of fluorescent proteins, they found that adding a triple nuclear localization...protease allows a transcription factor to enter the nucleus and modulate transcription of the mCitrine gene...includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for...

...further improved, by adding a nuclear localization sequence (SV40 Large T nuclear localization sequence) and...sites. FRT sites and loxP sites differ at the nucleotide level but share an overall structure of 13 base...the fusions allowing Cre/FLP translocate to the nucleus where they exert their recombinase activity (Logie...

...fibroblasts and keratinocytes to peripheral blood mononuclear cells (PBMCs). PBMCs isolated from whole blood...behave as separate extrachromosomal elements in the nucleus of the target cell. The method also reduces biosafety...Episomal vectors are based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA-1) system where each vector... peripheral blood mononuclear cells...

...target sequence was digested by sgRNA guided Cas9 endonuclease, homology dependent repair (HR, homologous recombination...however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice....off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases." Genome research 24.1 (2014): ...Daisuke, et al. "Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9...

...can be caused by intracellular factors such as nucleases and reactive oxygen species, or external forces...: The 5’ DNA end of the break is resected by nucleases to create a ssDNA 3’ overhang. This will serve...ssDNA templates (referred to as ssODNs (oligodeoxynucleotides)) are commonly used for smaller modifications...alleles using long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (...

...came epigenome-engineering tools - zinc finger nucleases and TALENs fused to epigenetic modifiers enabled...Christopher Newgard’s Lab has fused LSD1 to the smaller nuclease-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited... expression and dCas9-based epigenome editing. Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093...CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Research 44:5615–5628 . https://doi.org/10.1093...

...CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP) complexes of Cas9 protein and the separated...alleles using long ssDNA donors and CRISPR ribonucleoproteins.” Genome Biol. 18 (2017) (1):92. DOI: 10.1186...situ germline genome engineering using CRISPR nucleases. Genome Biol. 19 (2018) (1):25. DOI: 10.1186/s13059...Homology Directed Repair Learn about Cas9 Ribonucleoprotein (RNP) Delivery Resources on Addgene.org ...

...incorporate multiple single-strand DNA (ssDNA) oligonucleotides (oligos), and thereby rapidly create desired...transient suppression of DNA repair during oligonucleotide integration, allowing MAGE in otherwise unmodified...2016. PubMed PMID: 26884157 Aronshtam, et al. Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking... the IL6ST and BACH2 promoters. Vojta A, et al. Nucl. Acids Res. 2016. PubMed PMID: 26969735 Updated...26912792 FusX-TALEN assembly system TALEs as nucleases (TALENs) are genome-editing tools that are deployed...fusion proteins in a target cell. In addition to nucleotide-based linking strategies, post translational ...

...uses the direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates...non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without...recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093...elegans by CRISPR-targeted homologous recombination. Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093...

... Cas3. Cas3 functions as both a helicase and a nuclease, unwinding and cutting up DNA after recruitment...consider to be an integral component of CRISPR — a nuclease effector. However, IV-C does in fact have a helicase...Classification and evolution of type II CRISPR-Cas systems. Nucleic Acids Research, 42(10), 6091–6105. https://doi.... and involved in competition between plasmids. Nucleic Acids Research, 48(4), 2000–2012. https://doi.org...

...enhance HDR repair using a single-stranded oligodeoxynucleotide HDR template covalently attached to the ...the Cas9-gRNA RNP complex via the fused HUH endonuclease PCV. This increases HDR efficiency up to 30-fold...meningitidis, Nme2Cas9, that recognizes a simple dinucleotide PAM (N4CC), lending to high target site density...

...experiments of Kim and colleagues using zinc finger nucleases to harness non-homologous end joining repair (...chromosomal deletions in human cells using zinc finger nucleases. Genome Res. 2010 Jan;20(1):81-89. PubMed. 2. ... interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells. J.Biol.Chem. 2014 Aug...

...its endonuclease activity to generates a ssDNA substrate which is then nicked by its endonuclease domain...of Rep78 and Rep68 contains a DNA-binding and endonuclease domains, all four Rep proteins share a common...

...activation. Multiple nuclear localization signals (NLS) increase protein import into the nucleus. In developing...-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, they found that SunTag marked...

...By mutating one of two Cas9 nuclease domains, researchers created the CRISPR nickase. Nickases create...insert an EcoRI site using a single-stranded oligonucleotide (ssODN) donor. Using a D10A nickase to insert...small insertions or tags (ssODN; IDT Ultramer® Oligonucleotides) Use 100 nt homology arms for large insertions...

...genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted to elucidate...CRISPR loci to generate RNA, which guide a Cas nuclease to the specified exogenous genetic material next...mutation in the PAM sequence, preventing the Cas nucleases from re-identifying it (3). Surprisingly, few ...

... Cas9 and a gRNA, which subsequently enter the nucleus and cleave the specified target. Figure adapted...expression is by nature transient, which may lower nuclease toxicity or the potential for off-target effects... Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22 nuclear homing an artifact? Lundberg M, Johansson M. Nat...

...of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% knockdown efficiency...CRISPR-Csm presents an attractive alternative for nuclear-RNA targeting, where siRNAs are ineffective and...expression changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted...

...editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated with...they purified HF-BE3 protein for delivery in ribonucleoprotein particles (RNPs) to both zebrafish embryos...species. This toolkit includes TALEN and CRISPR nucleases, nickases, and gene activation systems as well... the amount of time that Cas9 is active in the nucleus to reduce off-target gene editing. The bacterial...

...of anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-...anti-FLAG-FB-GFP labeling various targets with nuclear (top, 4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin... editing, while gene editing can be done via a nuclease-active CasMINI. This small yet powerful system...