We narrowed to 447 results for: PAN
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TypeBlog PostPublishedJune 27, 2024, 1:15 p.m.... are already reserved for other markers in your panel, and thus cannot be used, you may want to look at...flexibility when it comes to fluorophore selection for panel design. Find streptavidin plasmids and biotin plasmids...advantage of this system is increased flexibility for panel design. Another perk is that one primary antibody... be different from the other antibodies in your panel. Otherwise, your secondary antibody will bind to...
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Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m....been shown to transduce skeletal muscle, cardiac, pancreas, smooth muscle, CNS, kidney, and retinal cells...been shown to transduce liver, skeletal muscle, pancreatic, and retinal cells (Issa et al., 2023). AAV9 ...pUCmini-iCAP-AAV9.452sub.LUNG1 Goertsen et al., 2022 Pancreas AAV6, AAV8 Wang et al., 2006 Retina 7M8 Dalkara... J., & Xiao, X. (2006). Widespread and Stable Pancreatic Gene Transfer by Adeno-Associated Virus Vectors... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells Note: This is not an extensive...Cy dye-nitrilotriacetic acid (NTA) conjugates (Kapanidis, Ebright, & Ebright, 2001). These Cy/NTA conjugates... et al. "Designing logical codon reassignment–Expanding the chemistry in biology." Chemical Science 6.1...Science281.5374 (1998): 269-272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs N., Yon W. Ebright, and Richard H. ... -
AAV Packaged on Request is Here!
TypeBlog PostPublishedJan. 10, 2025, 1:01 p.m....AAV Packaged on Request, an expansion of our viral vectors service, has officially launched! As of today... excited for all the ways this much-requested expansion will allow us to better serve our community. If...If you’re curious about the service, read on. Expanding our viral vector service Addgene has offered in-stock... -
2022 and 2023: Reflections and Looking Forward
TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m....learning to navigate the changes brought about by the pandemic. As we adjust, we are reflecting on how to help...information sharing, and research reproducibility while expanding our suite of materials and services to support...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....COVID-19 research. With the COVID-19 pandemic, we saw the need to expand reagent sharing even broader and ...Tsang These tips were written early on in the pandemic. Later in the year, Joanne Kamens followed up ... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m....As the world has battled the COVID-19 pandemic over the past few months, the scientific community has...research. The new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not...care and public health for this current COVID-19 pandemic, and outbreaks that have yet to come,” said the... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....Expanding your network of relationships early and often is the most effective tactic a scientist in training... next steps and follow up especially in large companies. It is fine to address the cover letter “To Whom...If the organization is small, any person at the company might be able to help you. If the organization ... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....physiology, development, and disease. In order to expand their model’s impact, organoid users have recently...months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9... INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m.... Society, or maybe you think about a local food pantry or community arts organization. Many people are...level of positive revenue to build reserves so the company is sustainable. The difference is what nonprofits...-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...other disciplines, over time. The resource may also expand to include other affinity reagents, such as nanobodies...creating an array of educational materials to accompany our antibody collection. Addgene is sharing our... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....build and grow the gene therapy division of the company. Ultragenyx’s goal is to develop novel products...annotated reagent.” He’s also interested to see Addgene expand the sharing platform to other reagents in biology...grants, or setting up commercial and nonprofit companies. His lab focuses on gene therapy work for genetic... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....for light mediated neuronal control and we are expanding our viral service to make these readily available... experiment. The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes... is vital in studying neural networks. We have expanded our calcium sensor collection to include our first... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m.... life science researchers. Many biotechnology companies are looking for PhD or Master’s level scientists...career?”, "Have they sought out opportunities to expand their skill sets?", “Have they succeeded in what...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m.... in our first study, but we're planning to soon expand to other cell types, including more physiologically-relevant...we’ve all been affected in various ways by the pandemic, it is clear that we have to stick together (6...Addgene, who will be taking over the distribution to expand the network of scientists working on this even ... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as pichia). What...general research community. Phillips Petroleum Company first commercially developed K. phaffii in the ...Since then, multiple biotech and pharmaceutical companies (along with some research laboratories) have picked... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....your antibody out on the bench overnight. Do not panic! Typically, antibodies are okay if they’re left ...excitation by a laser, which is then read by a detector panel in the machine. Fluorophores are light sensitive...you’ll likely be able to create a small, usable panel. First, fluorophores vary in brightness. You don... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....situation is this more true than the current global pandemic in which scientists race to find vaccines, therapeutics...to help find a faster solution to the COVID-19 pandemic. Though we’ve confirmed the expected features... science move faster in the middle of a global pandemic. And the reach of these plasmids is indeed global... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m.... the project. I was invited to participate on a panel about “Social Media for Science Communication” at... know how I was selected to participate on this panel, but my consistent writing for the Addgene blog ...Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences, and offers... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....into a plasmid, you can send your plasmid to a company. For a few hundred dollars, they will inject the... your strategy and the services offered by the company, you might have to map into which chromosome the...can be dissected at various times throughout the lifespan of the fly to determine the implications of your...