We narrowed to 181 results for: PID
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TypeBlog PostPublishedJune 24, 2014, 3:59 p.m....reaction compared to using a high-intensity laser to rapidly excite GFP. As a result of the different mechanisms...
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Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m.... in E. coli, SLIC is a cheap, standardized, and rapid multi-part DNA assembly method. Read on to learn... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient gene delivery... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....and faster ABEs (Fig. 2). These systems allow for rapid continuous protein evolution through many rounds... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....against many types of molecules including proteins, lipids, and carbohydrates, in this blog post we’ll focus... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....of commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. Thus, we are constantly... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m....tires give a better grip in the cold, but wear out rapidly in warm weather. Psychrophilic enzymes face very... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....their field, the answers were primarily negative. Stupid, overwhelmed, discouraged, tired…words that indicated... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....inducer, like Tet systems, may be activated very rapidly. In contrast, promoter systems that require transcription... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....prokaryotes. This includes but is not limited to: rapid growth, ease of replica plating and mutant isolation... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m.... desired 15 base pair sequence in the genome in rapid fashion. The two remaining plasmids in the kit have... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs and... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m....in the nucleus and is a good option if you need rapid transgene expression, since you don’t have to wait... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient purification of native histidine-tagged... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....indicator toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....asapbio.org/preprint-info ASAPbio, Preprints and Rapid Communication of COVID-19 research: https://asapbio.org... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....Forostyan, T., Colosimo, L. K., Graham, T. E. (2015). Rapid parallel measurements of macroautophagy and mitophagy...