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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 342 results for: SEF;

Showing: 101 - 120 of 342 results

  2. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ... we’ve got you covered! Here you will find some useful tips to help you make your plasmid construction...easy-to-understand guided manner. These videos can be especially useful when designing new primers to clone your sequence...

  3. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ... alongside these antibiotics just fine. This is useful for keeping your eukaryotic cell cultures bacteria-free...resistance to selection toxins. Auxotrophic selection is useful for producing stable mammalian cell lines, but ...

  5. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...either a frameshift or 21+ bp indel. This score is a useful measure to determine how many of the contributing...either a frameshift or 21+ bp indel. This score is a useful measure for those who are interested in understanding...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...information from a variety of fields in order to provide useful solutions to complex challenges. As a cross-disciplinary...attention for their work, a video abstract may be a useful tool for turning video views into online article...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... hold large amounts of data. Although it may be useful to selectively bring particular items into an ELN... from repositories back into ELNs would also be useful, so that the data can be viewed – and conceivably...

  9. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...your work out to other scientists who will find it useful, I highly recommend doing an AMA! Before I turn...have a lot of links and content at hand in three useful places: I had posted content on many of the requested...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... to bind and cleave target RNAs, although its usefulness in bacterial cells will likely be limited by ...molecule (like dCas9) suggests that dCas13a may be useful for isolating specific RNA sequences from a population...

  11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...bind a variety of PAM sequences, which makes them useful when no suitable SpCas9 PAM sequence is present... have other characteristics that make them more useful than SpCas9. For example, Cas9 from Staphylococcus...

  12. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...something that has fallen out of fashion but is still useful and most importantly, effective. In the research... for gene silencing. RNA interference RNAi is a useful tool to investigate the roles of specific genes...

  13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...expression in non-dividing cells IDLVs can also be useful for transducing non-dividing cells, as they avoid..., 2009).  Now that you know how IDLVs could be useful in your own research, we wish you the best of luck...

  15. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... the three toxins so that at least one might be useful in a broad array of pathogenic Proteobacteria. ...: AEM-00990. PubMed PMID: 31201277. Liljeruhm, Josefine, et al. "Engineering a palette of eukaryotic chromoproteins...

  16. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...from ATP to urotensin. The MTRIA sensors will be useful for investigating neuronal signaling and for engineering...pages to find more. We hope these tools will be useful in your future research, and we’re looking forward...

  17. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...-targeting field. Mice have long been extremely useful in the lab – they are relatively small and easy...generating mouse models Although CRISPR is incredibly useful for generating mutations by NHEJ and generating...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...Accelerate research and discovery by improving access to useful research materials and information. Many of our...responds to changes in intracellular calcium and is useful for direct imaging of events in cells and brain...

  19. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...and thoroughly controlled experiments.  Broadly useful controls Positive and negative controls Most biological...experiments. We hope that this blog post will be a useful starting point for you when designing rigorous ...

  20. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...membrane and initiate an action potential - a very useful tool for neuroscientists! Since then, scientists...Gong et al., 2020). Light-gated ion channels are useful when you want to activate or inhibit specific neurons...
Showing: 101 - 120 of 342 results