...can optimize your growth time, temperature, and shaking speed to maximize cell density and plasmid yield... total culture volume and you should ensure the shaker speed is fast enough for sufficient gas exchange...culture. Generally, large cultures in flasks may be shaken around 220 RPM, but smaller cultures, especially...especially those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn...

...research, the MoClo Kit she and Sylvestre Marillonnet share with the scientific community via Addgene, the struggles... it include? Patron: Sainsbury has a mandate to share and educate and enable plant science in general....editing plant genomes. We will definitely plan to share those tools with the plant community. We’d like ... whose tweets I find really inspirational. They share experiences, articles about women in science, and...

...When Karl Barry Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, ...mentors pass on more than just technical knowledge, shaping their trainees’ very way of thinking too. Albert...interest to other researchers in their field. Indeed, sharing results with the broader scientific community represents...serviceman in the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent...

...If you're interested in sharing your science, one of the big questions you'll be faced with is "how?"...it works so well for you, of course you want to share it with others! After all, won't it work just as...for longer educational videos, and Twitter, for sharing updates, new deposits, and new blog posts!  ...  of proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the ...

...other groups. First within my department where we shared materials and equipment between labs. Later I worked...PostDoc associations and specific projects where you share methods and equipment between lab groups and departments...twitter and maintain a connection by commenting and sharing. LinkedIn or ResearchGate allow you to send personal...events, articles and newsletters I make sure to share them with a short note - this creates a thoughtful...

...than DNA substrates. In terms of structure, Cas13a shares no homology to the most commonly used CRISPR enzymes...The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a) to begin to characterize this new effector... the CRISPR field. We feel that the culture of sharing within the CRISPR community is an essential part...and as always, we appreciate the opportunity to share these invaluable reagents with the scientific community...

...advantage of the EnvA-TVA receptor-envelope system (Wickersham et al., 2007). This system requires first supplying... and jump the synapse to the previous neuron (Wichersham et al., 2007). However, once rabies dG enters...https://doi.org/10.1128/jvi.71.8.6174-6178.1997 Wickersham IR, Finke S, Conzelmann K-K, Callaway EM (2006...Methods 4:47–49 . https://doi.org/10.1038/nmeth999 Wickersham IR, Lyon DC, Barnard RJO, Mori T, Finke S, Conzelmann...

...molecular language shared by every living thing fit onto a single lecture slide. That shared language is the...Some antibiotics block important cellular pathways shared by bacteria and eukaryotes. For example, puromycin...protein synthesis across kingdoms by targeting a shared aspect of ribosomal function. A puromycin resistance...

...story. Figure 1: Addgene’s Agile Approach Sharing is caring There are many moving parts to our software...diversifying our own knowledge of the software, sharing knowledge allows for life to happen without impacting...only thing you need to make a cake. The flavor, shape, and texture of a cake depends on how you combine...and discovery. We work with transparency, gladly share our knowledge, and love to collaborate, which, incidentally...

... Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the...plasmids he wanted. Addgene makes it so much easier to share your constructs from lab to lab. Promega supports...innovative tools. Afterall, isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic ...

... says “internationalization” young hearts often shake with the fear of uncertainty. However, internationalization...exist outside their immediate frontiers. Moreover, sharing a culture/country of origin with an international... help them carry out their plans. Through their shared experiences, mentors and mentees often developed...significant. Doubts, fears, insecurities… all shrink when shared with a mentor who has experienced them before and...

...Using acrylamide instead of agarose gives much sharper bands. I compared two MOIs of gRNA transduction... fragments, run PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell...available techniques and how quick and easy it is to share information and regents, it’s exciting to think ...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...

...Throughout the party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments...you all so much for an amazing twenty years of sharing science!       ...

...Creator: Tom McFadden Using Video to Share Your Science: We Share Science Additional Resources on Addgene.org...gained along the way to ourselves so we decided to share some of our tips in our new series: Addgene Lab ...

...that can help: Antibody data repositories, which share validation data, and antibody search engines, which...antibodies from many vendors, while data repositories share validation and experimental data to help you decide...application-specific ones to anything goes. We've shared the ones we're familiar with in Table 1.  Table...

..., developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve T cells....Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested..., Hacohen, N., Doench, J. G., LaFleur, M. W., & Sharpe, A. H. (2024). Framework for in vivo T cell screens...

... depositor, but it can also be valuable data to share in grant proposals or to demonstrate impact in annual...standardized way. Since Addgene encourages the public sharing of aggregated data only, information on requesting...you find the dashboard useful when analyzing and sharing your own depositor metrics!  About the Author Joachim...

...look forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9...PMCID: PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...

...focused on our core mission to promote scientific sharing. Listen to learn more about Darrell, his research...practices and promotes open science in the lab - sharing reagents, cell lines, data, and knowledge without...Addgene board of directors - increasing open source sharing and helping us stick to our core mission. Chapter...

... scientists from around the world and help them share their lab experiences, findings, and experiments...Neurodegenerative and brain disorders are frequently overshadowed by other medical conditions, so these topics...welcome researchers from all fields of science to share their experiences and explain what they study or...