We narrowed to 184 results for: SIR

... proceeded to study orthologs from Eubacterium siraeum (EsCas13d) and Ruminococcus sp. (RspCas13d), while...

... also high off-target activity, which may be undesirable in many applications. At the time of our first...

... another way, only cells or organisms with the desired phenotype will survive and be assayed at the end...

...before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some of these synthetic...

...mutagenesis. During this process, incorporation of a desired mutation into your plasmid of interest by PCR generates...

...guide: https://www.addgene.org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion ...

...essential proteins (when knockouts aren’t viable). siRNA or RNAi are small, double stranded RNA molecules...

.... References 1. Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott, M., & Devenish, R. J. (2008)....

...Their His tags allow for further purification, if desired, but it's not required for their use. Each RANbody...

...transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both...

...thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman’s vast network...

...ideally be optimized to promote generation of the desired product while avoiding the accumulation of toxic...

...them construct “designer” channelrhodopsins with desired characteristics. The model was trained to predict...

...expression. This system could be used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown...

...using a resistance marker; however, it is often desirable to remove the marker after the initial selection...

...literature and offer educated insight into what the desirable features of future technologies should offer in...

...reverse transcriptase (RT) template including the desired edit. Figure 1: Prime Editing schematic....

...of a combination of multiple genes to achieve a desired effect. This is commonly used in plant biology ...

...the goal of ‘fixing’ mutations or introducing a desired edit, but this particular class of treatments has...

... and to work in a variety of applications. The desired specificity of your secondary antibody depends ...