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Showing: 101 - 120 of 339 results

  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...to your primary antibody. If you are trying to visualize the precise location of a protein in a tissue ...secondaries:conjugates complex. If you are trying to visualize a protein in low abundance on a Western blot, ...

  3. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...secondary antibodies may be indicated on occasion, usually after other signal amplification techniques have...on the signaling molecule solely asmeans of visualization. This means that if all the primary antibodies...

  4. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...independently from its host’s chromosome. Although we usually think of bacteria when we think of plasmids, both...manage this with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other...

  5. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...festivals. Or engage with non-scientists in an unusual setting: Discuss your latest research in a local...your cup of tea, and you are more of an audio or visual person. Why don’t you record a podcast or put some...

  6. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...systems use 3 plasmids; an envelope plasmid that usually encodes VSV-G, a packaging plasmid containing structural...gene are transfected into a packaging cell line, usually a derivative of 293T. Packaging cells are incubated...

  7. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...rabies virus (RABV) can be used in the brain to visualize how neurons are connected. Viral Vectors as ...neurotropic viruses emerged as powerful tools for visualizing neural connectivity (Nassi et al. 2015). The ...

  10. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...ubiquitous background signal that makes it hard to visualize the precise location of the protein or structure... solution because it allows for tagging and visualization of specific, individual proteins under endogenous...

  11. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses. Feng S ...Considerations There are a few special exceptions to the usual split-FP options. While the BiFC interaction is ...

  12. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... Physical versus functional titer Viral titers usually come in two flavors, physical or functional. Physical... amount of viral particles in a sample and are usually based on the presence of a viral protein, such ...

  13. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...advanced and finally, to expert.   Figure 1. Visual representation of common injection routes in mice...straightforward, as it targets large muscle groups (usually the thigh), though it is important to avoid injection...

  14. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...protein of interest is untagged and functions per the usual. In the presence of a specific protease inhibitor... initially developed as an affinity tag for visualization of proteins of interest. A fluorescent Halo ...

  15. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...the first steps in the application process and usually occurs in the few months leading up to applications...such-and-such university State how you found their lab (usually through the university website or, even better,...

  16. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...students in academia. Getting a drug to the market usually takes over a billion dollars and at least a decade...off as an industry postdoc since your funding is usually separate from a project’s or a team’s, it is possible...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...sure your cells don’t stay out too long. Cells usually have their comfort zone around 37 °C and 5% CO2...to pH. Anything between orange and light orange usually indicates that the cells have grown enough, are...

  18. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them with fluorescent... of use, it is important to note that bacteria usually cannot produce functional multi-domain mammalian...

  19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...immunofluorescence (IF) - a common method for visualizing molecules of interest within a cell or tissue...your sample type. Starting with a protocol that usually works in your system and then optimizing from there...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...are smaller antibodies.They are derived from an unusual type of IgG antibody called a heavy chain antibody...fluorophore conjugates are used, but these are usually more expensive than unconjugated primary antibodies...
Showing: 101 - 120 of 339 results