We narrowed to 351 results for: SUA

...switched R-mAbs, multiple targets could now be visualized in a single experiment, which had not been previously...especially exciting for scientists working to visualize multiprotein complexes.  Fig. 2. Multiplex...

... person.  In an interview setting, this means persuading an employer that your past work has prepared ...the world to do it.  In the lab, it might mean persuasively articulating why the results you've measured...

...with XbaI or ClaI restriction enzymes and gotten unusual or unexpected results? Or considered why DpnI will..., the figure below illustrates how sequence visualization software (we used Snapgene here) can help you...

...Agrobacterium-mediated DNA transfer, each GOI is usually expressed from a different plasmid, which may lead...protein binders can be customized for protein visualization, relocation, degradation, modification, and ...

...to your primary antibody. If you are trying to visualize the precise location of a protein in a tissue ...secondaries:conjugates complex. If you are trying to visualize a protein in low abundance on a Western blot, ...

...secondary antibodies may be indicated on occasion, usually after other signal amplification techniques have...on the signaling molecule solely asmeans of visualization. This means that if all the primary antibodies...

...festivals. Or engage with non-scientists in an unusual setting: Discuss your latest research in a local...your cup of tea, and you are more of an audio or visual person. Why don’t you record a podcast or put some...

...ubiquitous background signal that makes it hard to visualize the precise location of the protein or structure... solution because it allows for tagging and visualization of specific, individual proteins under endogenous...

...Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses. Feng S ...Considerations There are a few special exceptions to the usual split-FP options. While the BiFC interaction is ...

...independently from its host’s chromosome. Although we usually think of bacteria when we think of plasmids, both...manage this with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other...

...world to detect, purify, quantify, deplete, and visualize proteins of interest (Greenfield, 2014). Traditionally...Monoclonal antibodies produced from hybridomas are usually a single antibody recognizing one epitope. (Bottom...

...mismatches usually don’t affect the function of the plasmid.   Confirming the insert We usually confirm ...

...systems use 3 plasmids; an envelope plasmid that usually encodes VSV-G, a packaging plasmid containing structural...gene are transfected into a packaging cell line, usually a derivative of 293T. Packaging cells are incubated...

...rabies virus (RABV) can be used in the brain to visualize how neurons are connected. Viral Vectors as ...neurotropic viruses emerged as powerful tools for visualizing neural connectivity (Nassi et al. 2015). The ...

... Physical versus functional titer Viral titers usually come in two flavors, physical or functional. Physical... amount of viral particles in a sample and are usually based on the presence of a viral protein, such ...

...characterized and deposited a variety of constructs for visualizing cellular components (nucleus, endosomes, lysosome...and an epitope tag (ALFA-tag, or At) for easy visualization. A cleavage site for endogenous BACE1 protease...

...protein of interest is untagged and functions per the usual. In the presence of a specific protease inhibitor...developed by Promega as an affinity tag for visualization of proteins of interest. A fluorescent Halo ...

...advanced and finally, to expert.   Figure 1. Visual representation of common injection routes in mice...straightforward, as it targets large muscle groups (usually the thigh), though it is important to avoid injection...

...the first steps in the application process and usually occurs in the few months leading up to applications...such-and-such university State how you found their lab (usually through the university website or, even better,...

...students in academia. Getting a drug to the market usually takes over a billion dollars and at least a decade...off as an industry postdoc since your funding is usually separate from a project’s or a team’s, it is possible...