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We narrowed to 351 results for: SUA

Showing: 101 - 120 of 351 results

  1. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ... Alt text is used by screen readers to provide visually impaired visitors with information about images...words to describe the image in a way that helps visually impaired readers receive the same information ...

  3. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...with XbaI or ClaI restriction enzymes and gotten unusual or unexpected results? Or considered why DpnI will..., the figure below illustrates how sequence visualization software (we used Snapgene here) can help you...

  5. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...incorporated at the termini of the crRNA molecule (usually 1-3 of the first 3 bps are modified) for optimal...RNA in your targeting experiments allows you to visualize transfection efficiency and even track localization...

  6. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...to your primary antibody. If you are trying to visualize the precise location of a protein in a tissue ...secondaries:conjugates complex. If you are trying to visualize a protein in low abundance on a Western blot, ...

  7. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...secondary antibodies may be indicated on occasion, usually after other signal amplification techniques have...on the signaling molecule solely asmeans of visualization. This means that if all the primary antibodies...

  8. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...systems use 3 plasmids; an envelope plasmid that usually encodes VSV-G, a packaging plasmid containing structural...gene are transfected into a packaging cell line, usually a derivative of 293T. Packaging cells are incubated...

  9. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...rabies virus (RABV) can be used in the brain to visualize how neurons are connected. Viral Vectors as ...neurotropic viruses emerged as powerful tools for visualizing neural connectivity (Nassi et al. 2015). The ...

  10. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...festivals. Or engage with non-scientists in an unusual setting: Discuss your latest research in a local...your cup of tea, and you are more of an audio or visual person. Why don’t you record a podcast or put some...

  11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...ubiquitous background signal that makes it hard to visualize the precise location of the protein or structure... solution because it allows for tagging and visualization of specific, individual proteins under endogenous...

  12. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...independently from its host’s chromosome. Although we usually think of bacteria when we think of plasmids, both...manage this with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other...

  13. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses. Feng S ...Considerations There are a few special exceptions to the usual split-FP options. While the BiFC interaction is ...

  16. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... Physical versus functional titer Viral titers usually come in two flavors, physical or functional. Physical... amount of viral particles in a sample and are usually based on the presence of a viral protein, such ...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...sure your cells don’t stay out too long. Cells usually have their comfort zone around 37 °C and 5% CO2...to pH. Anything between orange and light orange usually indicates that the cells have grown enough, are...

  18. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them with fluorescent... of use, it is important to note that bacteria usually cannot produce functional multi-domain mammalian...

  19. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...advanced and finally, to expert.   Figure 1. Visual representation of common injection routes in mice...straightforward, as it targets large muscle groups (usually the thigh), though it is important to avoid injection...

  20. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...the first steps in the application process and usually occurs in the few months leading up to applications...such-and-such university State how you found their lab (usually through the university website or, even better,...
Showing: 101 - 120 of 351 results