We narrowed to 490 results for: Star;

...expression, is it necessary to add a G nucleotide to the start of my target sequence? A3: The human U6 promoter...promoter prefers a 'G' at the transcription start site to have high expression, so adding this G could help ...when they order the oligo. If your spacer sequence starts with a 'G', you naturally have one and do not need...that mutation be selected/screened? A13: Before starting your experiment, consider co-transfecting with...

...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ...We borrowed an idea from the software folks and started Hack Days. During Hack Days, teams at Addgene will...Executive Director, Joanne Kamens, responded by starting something we call “Random Lunches.” Once or twice...

... pathways just waiting to be discovered. As we started building, we thought – how can we make the connection...science video process One major challenge when we started was creating a LEGO stage that resembles a real...in a custom made, colorful laboratory. When we started, the hardest part of the process was to embed personality...

...States Postal Service picks up mail. NEB actually started this program in 1976! Millipore Sigma takes back...cost of regular waste services. Learn about how to start a glove recycling program at your institute! What...culture room for the wrappers and ship them back. Start a glove recycling program at your institute! Universities...

...AAV.  Where do you start? Plasmids of course! You just need three plasmids to start making the AAVs you...Alternative splicing and differing translational start sites generate the three VP proteins. The three ...

...stop you. Where do you start? Turns out like all good experiments, making AAV starts with some plasmids. .... You just need three plasmids to start making AAV:  the packaging plasmid which contains the structural...

...another person about their career path is a great start to a mentoring relationship, but if you don’t take...list available from this post. Use an activity to start a discussion. The Forced Choice Analysis is a fun...clarify and discuss values. The activity is just the start – it is the discussion after that will lead to learning...

... questions,” says Zhang. “Usually the moderator starts by asking the speaker the highly upvoted question...chats between sessions or waiting for a session to start. “There was not much time to network,” Zhang says...discussing at tables and the event hasn't even started yet! @neurosymp will be interesting this year. ...

...give rise to a starfish shape. You can ask me about the different arms of this starfish sometime, but... plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne Kamen's LinkedIn...

... you. 2. Gibco Cell Culture by Gibco Whenever I start to learn a new technique, I try to look for a resource...for a mass spectrometry app. I digress. When I started learning proper cell culture technique, I tried...manual. Overall, it’s a great tool for someone starting cell culture work. 3. NEB Tools by New England...

...screens to answer their own research questions. Starting at the Broad Institute For his post-doc, Doench... technology development alongside it.” Doench starting working with David Root in the then RNAi Platform...left him a bit skeptical of the technology at the start of the CRISPR craze, but he says, “In the case of...

.... 1: Open source hardware builds   Since I started my PhD, I have been convinced that most microscopy...science. Researchers around the world don't have to start from scratch every time to pursue their scientific...IPHT Jena. He is co-founder of the open-hardware startup “openUC2” and focuses on bringing cutting-edge ...

...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...represents Addgene’s largest new initiative since we started distributing plasmids in 2004. We’ve already distributed... 501(c)3 nonprofit and our plasmid service was started based on donations. That seed money was used to...

...computer at the command line, and so was really starting from the beginning. Luckily, a fellow graduate...that it is helpful to those of you just getting started. The practices suggested here may seem tedious ...code does are very useful for those that are just starting out, and helps clarify the role of the code. ...

...do so. Immediately after the injunction, as we started to hear of institutions cancelling plans to raise...across geographical regions When we originally started gathering this information, many assumed that those... receiving data from our FOIA requests, we are starting to see very interesting data on how much postdocs...

...own to share reagents. I love the way scientists starting their own labs get excited about making their ...love working with local organizations like Just-a-Start, the Northeastern co-op program, and with other ...scientists around the globe and our own staff is starting to reflect this diversity, remarkably so for a...

...as they were deposited before 2017 when Addgene started sequencing all incoming plasmids. If you are interested...Addgene’s QC team. Email [email protected] to get started! Addgene’s QC process Addgene prioritizes the sequence...replacement for the plasmid, and the full QC process is started over again after receiving the replacement plasmid...

...of the fluorescent protein (i.e. rearranging the start and end positions without changing the order of ... FRET for imaging biomolecules in live cells (Sustarsic and Kapanidis 2015), which may lead to monitoring...18479457. PubMed Central PMCID: PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the...

...Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection of plasmids for coronavirus... of cathepsin S, L, and B. This became a great starting point for SARS-CoV-2 researchers to investigate...Van Dis E, Catamura C, Yamashiro LH,  Begeman A, Stark JC, Shon DJ, Fox DM, Puschnik AS, Bertozzi CR, Carette...

... addition to sequencing plasmids, we have also started using NGS to check the quality of other services...quality data! Addgene's new in-house NGS process To start, the process is only made possible by the use of...quickly create Illumina sequencing libraries. From start to finish the library preparation process only takes...