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We narrowed to 542 results for: TIL

Showing: 101 - 120 of 542 results

  1. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...” identified seven types of difficult people: Hostile-aggressives, who are usually antagonistic and impolite...is a difficult person, the above strategies will still be helpful but you will need to be more assertive... try to intimidate you with negative comments (hostile-aggressives, complainers, know-it all experts, ...

  2. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...done the technical design of your blot, but are still finding yourself having issues, you’re in the right...blocking conditions on the spec sheet. If you’re still seeing high background after following manufacturer...yourself. But even with a validated antibody, it can still take time for you to get it to work well. Dilutions...antibody dilution. Adjust the antibody dilution until your protein dilutions are in the dynamic linear...

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...the pandemic; for more discussion on that, see Pyhtila et al 2023.)  One of our favorite observations ...CRISPR Plasmids by Addgene,” in The CRISPR Journal (Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou...publications.  References and resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. ...

  4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...frequency. Despite the progress we’ve made, there is still a need for and interest in enzymes with shorter ...editing; for known promiscuous gRNAs, xCas9 3.7 still showed a 4.2-9.4-fold improvement in off-target:...these early data are exciting, there’s a lot we still don’t know about xCas9. “I’m not 100% sure xCas9...

  5. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...after a semester while the other student stayed until he graduated. Years later I attended a formal training...team members by giving them new opportunities. I still need to check myself sometimes to make sure I am.... In many cases reports are not aware of issues until you bring them up. I wouldn’t go as far as saying...

  6. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Chemigenetic sensors take a hybrid approach and utilize both a genetic component as well as a small molecule...fluorescence all have room for improvement. These sensors still have many useful applications, though, and if you...this two-part fluorescent system, and rhodopsin still functioning as the voltage sensor. FP-rhodopsin ...

  7. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...across multiple model organisms (3) lies in the versatility of its delivery methods. Researchers can conveniently...unhappy with the new method and 65% wanted to wait until someone else optimizes an NgAgo protocol for their...complex with a 5’-P-ssDNA guide at room temperature. Until that time, those who have been lucky with NgAgo ...

  9. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ... the base of the stem. This buys you a few days until new inflorescences begin to grow. This will also...too old to transform. This transformation could still work, but the efficiency will be low. Tips: Use...plants: If you transform early, your plants will still have plenty of time to produce seeds after you expose...

  10. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...easiest way to make a knock-in cell line is to utilize the built-in repair pathway cells already have ... multiple rounds of cleavage at the target site until either the PAM site or part of the guide’s recognition...the most common editing pathway for DNA repair. To tilt the scales in your favor for an HDR edit, you can...

  11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...ll prevent binding to any target protein that is still on the inside of the cell. However, assuming you...hidden epitopes. However, keep in mind that you will still contend with many of the same drawbacks to each ...early steps don’t even involve antibodies, they are still critical to the outcome of your IF experiment and...

  12. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...available to researchers around the world. While we still offer many resources for producing vectors from ...vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023, we shared 93,491... Rego, M., Wang, C., Zhang, Y., DeMaio, K. H., Tillgren, M., Fava, A., Clouse, G., Patrick, M., Guerin...

  13. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...post:  New compact, efficient prime editors A versatile and precise system to improve on-target delivery...10.1016/j.cell.2023.07.039. PMID: 37657419.   A versatile and precise system to improve on-target delivery...lab recently made two deposits that increase the utility of this technique.  By engineering a light-sensitive...

  14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre recombinase. FLPe codon ... Ligand regulated recombinase - keeping Cre out until needed But what can be done when the recombination...Cre/ FLP versions are retained in the cytoplasm until estrogen receptor ligand Tamoxifen is added. Tamoxifen...

  15. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...receptor (AAVR). Cell lines lacking galactose were still transduced by PHP.B but only if Ly6a was expressed...with PHP.eB, an enhanced version of PHP.B, virus still reached the brain endothelial cells of these mice...capsid variants could unintentionally limit the utility of candidate capsids.  Are you interested in using...

  16. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...to recognize my transferable (or “soft”) skills until I was out of the lab and in an office environment...you your next job or promotion. Also, don’t wait until you’re out of the lab to think about these--get ...into science in the first place. Finally, if you still find the idea of not holding a pipette again upsetting...

  17. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...nucleic acids. Read on to learn more about this versatile technique.  Immunoprecipitation Overview An immunoprecipitation...typically carried out in one of two ways. Both methods utilize the same basic strategy: immobilize the antibody... One of the most common immobilization methods utilizes bacterial cell wall proteins, Protein A and Protein...

  18. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...technique is still used today to test for HeLa contamination. HeLa contamination is still a problem today...You would never thaw any of the Master cell vials until all of the working vials have been used up. This...

  19. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...generation of base modifiers available. This system utilizes an RNA template containing the desired edit adjoined...like with base editing, alternative Cas9s can be utilized allowing for PAM flexibility. Fig. 2: Prime...Don’t want to permanently modify the genome but still want to affect gene expression? dCas9 can be used...

  20. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...many expression plasmids utilize inducible promoters, which are 'inactive' until an inducer such as IPTG...Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression F- ompT lon hsdSB (rB...
Showing: 101 - 120 of 542 results