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We narrowed to 340 results for: abo.4

Showing: 101 - 120 of 340 results

  1. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...see whether and to what extent they overlap. Figure 4 compares the spectra of enhanced GFP (eGFP) and FITC...be used within the same panel.     Figure 4: Excitation and emission spectra for eGFP and FITC...channel to use to detect eGFP. As you saw in Figure 4, eGFP strongly overlaps with FITC, hence reading out...spectrum is located in a range of wavelengths of about 380–700 nm. Photons of lower wavelengths are higher... 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were to use a laser that excites at...optimal excitation happens at higher wavelengths of about 750 nm. Source: https://www.bdbiosciences.com/en-us... we start building your first panel, we'll talk about one last principle of color emission. As you can...

  2. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...microenvironment produce CTL associated protein 4 (CTLA-4), a receptor that turns off T cell function upon...immunology 3.11 (2002): 991-998. PubMed PMID: 12407406. 4. Weiner, George J. "Building better monoclonal antibody-based...potential beneficiaries. Collaborative therapy For the most efficient outcomes, collaborative therapies must be... of the tolerance-producing antigens enunciated above. Finally, signals released from the microenvironment...are in their clinical trial phase. In all of the above therapeutics, techniques like genome engineering...are beginning to see the fruits of many years of labor with up and coming immunotherapy treatments. As ...clinic, physicians and researchers are learning much about how treatment regimens can be improved and how the...

  3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth review of...CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka, and John...the context of the cell. However, much knowledge about functionality can be gained by understanding the...subcellular marker proteins, it’s possible to get an idea about the subcellular structures your protein resides ...with. These “colocalization” studies give insights about the proximity of two proteins within the same subcellular...Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging in Human Cells...

  4. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    Published
    Oct. 5, 2017, 1:13 p.m.
    ... conversation with your manager early on. Chapter 4: Crystal's approach to management and the importance...therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight ...Chapter 2: What's a lab group in industry? The collaborative nature of working in industry and how delegating...metrics for success. The importance of thinking about the big picture and not just the next experiment...resources in our Mentoring for Scientists Series Learn about Turning Your PhD into a Career with Joanne Kamens...

  5. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the 3’ end of the target sequence... Find plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah...a cell culture model. What we still don't know about Cpf1 In their original paper characterizing Cpf1...Addgene Blog Catch Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac...

  6. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...capable of recruiting additional effector domains (4). Taken together, these minor gRNA alterations can...PMID: 26344044. PubMed Central PMCID: PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human genome engineering...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Listen to Our Interview with Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators...

  7. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...top ten countries for each group (Figure 4).    Figure 4: Addgene deposits and distribution by country...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....

  8. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...phosphorylated DNA termini to 3 ́-OH DNA termini (1-4). In vitro, ligases (notably T4 DNA ligase) are critical...PMID: 18518823. PubMed Central PMCID: PMC2933818. 4. Shuman, Stewart. "DNA ligases: progress and prospects... And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks in ...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...Cardiovascular Research, 1(4), Article 4. https://doi.org/10.1038/s44161-022-00046-4 Kumar, S. R., Miles, T... fold change in RNA and DNA levels (~25-fold and ~4-fold respectively) relative to AAV9 in the pigtail...nervous system. Current Research in Neurobiology, 4, 100086. https://doi.org/10.1016/j.crneur.2023.100086...106–115. https://doi.org/10.1038/s41593-021-00969-4 Goertsen, D., Goeden, N., Flytzanis, N. C., & Gradinaru...enhanced transgene expression in the macaque CNS. Med, 4(1), 31-50.e8. https://doi.org/10.1016/j.medj.2022.11.002... for their use. Then, we’ll provide information about commonly used and emerging capsids to help you decide...our Caltech Systemic Capsids page to learn more about that collection. Table 1: Systemic capsid plasmids...

  10. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...spread (metastasize) and turned into Stage 3 or Stage 4 cancer. These later stages of a cancer diagnosis indicate... is large in size and may have spread while Stage 4 indicates that the cancer has spread from where it...common cancers like lung (about 224,000 diagnosed this year) or breast cancer (about 246,000 diagnosed this...Mesothelioma Cancer Alliance. We have learned much about the causes of cancer and the different avenues that... asbestos exposure to the time of diagnosis) is about 35 years, but it usually ranges from 20-50 years... an orphan cancer; it is rare disease with only about 3,000 new mesothelioma patients diagnosed every ...from mesothelioma several years ago – recently collaborated with Senator Barbara Boxer to introduce the ...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...38 kb Cells HEK-293T Sf9 Time to collection 2–4 days 5–10 days Purification Ultracentrifugation ...frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077.... widely available, so you’ll only have to worry about cloning your transfer plasmid. The transfer plasmids...typical insect cell preparations, which we talk about below. Processing The viral supernatant is typically...available, so the amount of cloning required is about the same between insect and mammalian systems. Baculovirus...dead cells and cellular debris. While adding time (about three to four days), this second round provides ... can skip the first round of production, saving about a week of work. An important note is that this is...

  12. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ... ultimately getting interpretable results: Figure 4: Expression Level of Gene X   The design and selection...it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central PMCID: PMC3321166...optimization within your experiment. Read on to learn more about the various controls that can be used for plasmid-based...: Expression Level of Gene X The results shown above are from a single experiment wherein Plasmid A (...be compared. The Empty Vector Control (mentioned above) could also serve as an important negative control...experimental cells. For example, in the experiment above, Plasmid A contains an shRNA that targets human ... times and on different days. Using our example above, we would test different preparations of Plasmid...

  13. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...library consists of 77,441 sgRNAs with an average of 4 sgRNAs per gene and 1,000 non-coding control sgRNAs...PMID: 25307932. PubMed Central PMCID: MC4253859.  4. Konermann, Silvana, et al. "Genome-scale transcriptional...Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled...large-scale functional screens Read this blog post about genome-wide screening using CRISPR Resources on...libraries for the mouse genome! Other Resources Read about CRISPRi and CRISPRa from the NIH E-newsletter ...

  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...PMID: 25531807. PubMed Central PMCID: PMC4273606. 4. Improved vectors and genome-wide libraries for CRISPR...Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID: PMC4486245...gene therapy applications. Read on to learn more about the current (and future) applications of lentiviral...do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1, well-known to...Libraries Enable Genome-Scale, Knockout Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional...Addgene.org Check out Our Lentivirus Guide Learn about Virus Biosafety Find Lentiviral Vectors for Your...

  15. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...approach using a single crRNA array to express up to 4 crRNAs. Zetsche et al. also suggest that Cpf1 may ...Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the 3’ end of the target sequence...genome editing than Cas9 - read on to learn more about Cpf1 and check out our CRISPR guide for a refresher...mediated cleavage are 42-44 nucleotides in length, about the same size as Cas9’s, but with the direct repeat... Cpf1 was first identified, we didn't know much about its on-target and off-target editing efficiency....CRISPR system proved we had a lot more to learn about CRISPR biology. Later work, like the adaptation ...

  16. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...people have regular schedules. Work 8 to 5 or 7 to 4 or maybe 7 to 6 and 8 to 10 or whatever works for ...carefully and with attention to those around you #4 Thou shalt not bear the burden alone You, alone, are...on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work...worry less about how they will handle an emergency and this will make you much more confident about their ...

  17. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...specific amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon...PubMed PMID: 2265611. PubMed Central PMCID: PMC552224. 4. Gustafsson, Claes, Sridhar Govindarajan, and Jeremy...that lead to these preferences and what can we do about them? Read on to find out!   Why do organisms have...understood, but some possible reasons include: Metabolic pressures - it takes cellular resources to produce...amino acid I’d like to encode,” but, as described above, not every region of a protein should necessarily...Effects on transcription (remember, it’s not all about translation - e.g. codon choice may interrupt transcription...

  18. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...to indels (Anzalone et al., 2019). Figure 4: Editing the PAM prevents the prime editor from re-engaging... 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F... primer binding site, starting with a length of about 13 nt (Anzalone et al., 2019). Primer binding sites...of reverse transcriptase template, starting with about 10–16 nucleotides. For longer templates, it is even.... Test multiple nick sites, starting with sites about 50 bp upstream and downstream from the prime editing...

  19. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...harvested and purified. In total, this process takes 4-7 days, not counting the time needed to expand the...completed in one long day, or the virus can be stored at 4 °C and buffer exchanged the next day. Refer to the...a workspace with plasmid, not that I would know about this… A no template control (NTC) should always ... variability. Check out this post to learn more about different ways to titer AAV. Do you have any AAV...at Fred Hutchinson Cancer Center for teaching me about AAV production and purification! References 1. Aurnhammer...

  20. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...designed to detect resulting in false positives [2, 4]. Solutions to the polymer problem There are some ..., 1999. 17(10): p. 969-73. PubMed PMID: 10504696. 4. Ohashi, T., et al., An experimental study of GFP-...sold by Clontech and is in a freezer box in just about every lab in the world, is not inert. In all seriousness....11 mM [2]. Following the simple logic outlined above, you might think EGFP is unlikely to form dimers...affinity assays used in the papers describing the FPs above including molecular sizing columns and native gels...
Showing: 101 - 120 of 340 results