We narrowed to 523 results for: ato

...technology (Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods...tagging of proteins with mAID and other tags (Yesbolatova et al., 2019) using CRISPR. This toolkit makes...neurons. Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven...

...immunosurveillance. The presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from ...self-limiting effects kick in trying to resolve the inflammatory state by inducing immunosuppressive measures...Chimeric Antigen Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy is ...a researcher to control immune signaling and inflammatory responses remotely with spatiotemporal precision...

...heterologous envelope or a post-transcriptional regulatory element can be used for either type of retrovirus...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,...population. Some adenoviruses are known to cause respiratory disease across species, as well as cancer in ...strains have been engineered to induce a lower inflammatory response within hosts. Relative to the other...

...activation of keratinocytes, which release inflammatory mediators that trigger the activation of dendritic...Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect 0.27 to 4.6...IL-36 cytokines are a potent inducer of an autoinflammatory loop: keratinocytes both respond to and release...

...CRISPR for that, too! CRISPR activators (CRISPRa) are transcriptional activators fused to a dead Cas9. The...increase expression. There are several flavors of activators which can be used with this approach - read more...them on our CRISPRa blog! Fig. 1 CRISPR activator and repressor mechanisms Want to transiently...

...sword – balancing antiviral benefits with the inflammatory response associated with the introduction of...threshold and thus do not typically activate an inflammatory response. However, if dsRNA sensors are mutated...its way outside of the mitochondria, the same inflammatory response can activate. These types of cellular... disorders, including some forms of lupus and rheumatoid arthritis. These types of immune responses are...

...available to counteract the toxin. If, however, an investigator were to successfully clone an insert into the...insertion will completely replace ccdB with the investigator’s insert of interest. Hence correct clones are... behind the “swapping out” of ccdB with the investigator’s gene of interest.   CcdB resistant E. ...for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995 Mar;15(6):1031...

...used red fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus, T-DDOGs can...expression robustly activated the reporter gene TdTomato, whose expression was absent without electroporated...T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells with GFP, at a high activation...frequency of 56-93%. In the converse test, 98% of TdTomato expressing cells were GFP+, indicating a robust...

... infections along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality...understood.  In line with this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG...discovery led them to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential... 1939 for the treatment of botulism and Lambert-Eaton myasthenic syndrome (LEMS) because, at high concentrations...

...bone marrow (BM) cells, primary human hepatocytes, and hematopoietic stem cells (HSCs) using Nanoblades....deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the ...from GFP transgenic mouse  GFP  ~60-65% Human hepatocytes  Myd88  ~50% Human HSCs  Myd88  ~50% Table...

..., 50ms “off”.  A pulse generator such as the A.M.P.I Master-9 Pulse Stimulator, which can control output...cable is threaded through—and fixed to—an optical insulator called a ferrule (below). The fiber optic cable...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...

... bank account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab...business. We ended up applying to a business accelerator freely stating that we had an idea, knew how ...rough day, and we have great partners in this accelerator program who want us to succeed. We’re excited...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside...

...with Addgene by year. (C) Cumulative number of laboratories that have deposited CRISPR plasmids by year....nuclease into three functional groups: nucleases, modulators, and chimeric effectors. Nucleases generate a... a classic double-stranded break, while modulators regulate transcription and chimeric efforts make a ...deposited and distributed CRISPR plasmids. But modulators and chimeric effectors, made available in 2013...

...libraries to analyze pre-existing heterogeneity of hematopoietic stem cells and track clonal dynamics during ..., liver, CNS (limited penetration), retina, hematopoietic cells, and immune cells. B) AAV11 is reported...binds to AMPA receptor subunit GluA1 found at excitatory synapses (Rivera & Huang, et al., 2025). AF was...determined cells. This version of ATLAS works only at excitatory synapses; however, its modular design allows ...

...common use of intradermal injections is studying inflammatory responses, as this method is useful for analyzing... correct location requires a lot of skill and anatomical knowledge. Intrathecal injections are used for...Routes of administration. In H. Hedrich (Ed.), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, ...M. A. (2011). Administration of substances to laboratory animals: routes of administration and factors...

... J. (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https...insertion likely precipitated domestication of sweet potatoes (Kyndt et al., 2015)! It sounds simple, and it.... et al. (2015) ‘The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes...

...up with poor droplet quality. Using a droplet generator, the sample and the droplet generation oil are...Pipette slowly If you are using a manual droplet generator, you will have to be careful about transferring...transferring your droplets from the droplet generator to the PCR plate. Maintaining a high droplet count is important...sample. Even if you are using an automated droplet generator, you should avoid pipetting too quickly when making...

...m a father of three, a scientist and science communicator, and I strive to be a volunteer and contribute...scientist. When our elementary school needed a new coordinator for the annual Math & Science Night, I took the...contacting our school district’s science curriculum coordinator with minimal luck, but eventually the 8th grade...great feedback from teachers and other science communicators.  What can CRISPR do? I spent more time on...

...is the Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations...through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database...operations, she also works on improving laboratory efficiency through automation.       More Resource Avaliable...

...amount of grant funds available and the increasing regulatory process for grants has fostered the demand for...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...worked for a health department and then for the regulatory services department as our healthy homes program...