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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 137 results for: ckb

Showing: 101 - 120 of 137 results

  1. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... make your own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed...

  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples of potential...

  5. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... based on the copy number of the empty plasmid backbone or a researcher’s personal observations. If you...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...sequence, check the “Align two or more sequences” checkbox and paste your reference sequence into the Subject...

  8. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...immediate co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the...

  16. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...Resources on Addgene.org Collection of empty plasmid backbones ...
Showing: 101 - 120 of 137 results