We narrowed to 317 results for: cript

...backbones for constructing fluorescent fusions. Transcription Reporter: Placing GFP under the control of a...promoter in a given cell type. This type of transcription reporting was among the earliest uses of GFP... gene of interest, or expressed from the same transcript as the gene of interest, but after an internal...

...we probably exchanged dozens of drafts of the manuscript. This is the time to transform the document into...Resources on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing ...Organizing Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out...

...developed a separate script for manually inputting known innocuous differences. This script compares the sequence...Any sequences that are a perfect match from this script can then continue in the auto-align process. This...

...the library sample, and any other information or scripts provided by the depositing lab for data analysis...References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening - An excellent protocol...Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc. 2017 Apr;...

... with tRNAs, which are cleaved out of the RNA transcript by endogenous processing enzymes (Figure 1). ...processing only occurs on the RNA polymerase III transcript, the sgRNAs were encoded on the lentiviral minus...including modifications to the Cas9 or reverse transcriptase enzymes, inhibition of host cell proteins, and...

...maturation (Tillotson & Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have...have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...groove of AT-rich DNA sequences to regulate transcription (Baker et al., 2013). Mutations that affect ...

...protease allows a transcription factor to enter the nucleus and modulate transcription of the mCitrine ...activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed ...

...specialized strains are available to confer greater transcriptional control, assist with proper protein folding...Lacks functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm... the expression plasmid in order to repress transcription from a hybrid promoter. Although the DE3/T7 ...

...policy and recently announced that of the 16,000 manuscripts that had been processed since the declaration...access of this data is to be made at the time of manuscript submission. The rationale includes increasing...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

...strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to reduce transcriptional readthrough...worked too. Rhamnose induction relies on the transcriptional activator rhaS. To allow manipulation of bacteria...

...methyltransferase — that can drive lasting transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration...versions using dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen...Weissman, J. S. (2021). Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell...

... editor or adenine base editor) and reverse transcriptase-mediated prime editing technologies have been...Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation. Plant Physiol 169:971–985 . https...CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 ....

...amplified. RPA reactions also contain reverse transcriptase to produce cDNA. Isothermal amplification is...thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the amplified...First, RNA is extracted and subject to reverse transcription. This is then amplified by PCR using Taq Polymerase...

...pathological condensates, including oncogenic transcription, proliferation of leukemia cells, and assembly...easily visible chromoproteins in a cell-free transcription-translation system (Figure 6). This approach...CRISPRkit uses a cell-free system to carry out transcription and translation of chromoprotein and gRNA plasmids...

...Antibody-independent methods may include analysis of transcriptomic or proteomic data from several different tissues... feasible and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want...tissues. You know that there is a wealth of transcriptomic data from this sample type, so you use your...

... few prior publications to go on, a submitted manuscript could only have been viewed as a significant ...require working knowledge of the command line or scripting. Input flexibility: Software tools require some...machine. On the offline front, there is a Python script called SSFinder, but I find its implementation ...

... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding...can even be used to detect a single copy of a transcript. But this sensitivity can sometimes cause as ...

...Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to...to an Mxi1 repressor domain repressed transcription of HSP90 an essential C. albicans gene. Read the ...

...ability to direct enzymes – from nucleases to transcription factors – to specific sequences of DNA has become...of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014...

...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...