...the library sample, and any other information or scripts provided by the depositing lab for data analysis...References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening - An excellent protocol...Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc. 2017 Apr;...

...protease allows a transcription factor to enter the nucleus and modulate transcription of the mCitrine ...activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed ...

...we probably exchanged dozens of drafts of the manuscript. This is the time to transform the document into...Resources on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing ...Organizing Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out...

...maturation (Tillotson & Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have...have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...groove of AT-rich DNA sequences to regulate transcription (Baker et al., 2013). Mutations that affect ...

...specialized strains are available to confer greater transcriptional control, assist with proper protein folding...Lacks functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm... the expression plasmid in order to repress transcription from a hybrid promoter. Although the DE3/T7 ...

...policy and recently announced that of the 16,000 manuscripts that had been processed since the declaration...access of this data is to be made at the time of manuscript submission. The rationale includes increasing...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

...methyltransferase — that can drive lasting transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration...versions using dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen...Weissman, J. S. (2021). Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell...

...amplified. RPA reactions also contain reverse transcriptase to produce cDNA. Isothermal amplification is...thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the amplified...First, RNA is extracted and subject to reverse transcription. This is then amplified by PCR using Taq Polymerase...

... editor or adenine base editor) and reverse transcriptase-mediated prime editing technologies have been...Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation. Plant Physiol 169:971–985 . https...CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 ....

...strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to reduce transcriptional readthrough...worked too. Rhamnose induction relies on the transcriptional activator rhaS. To allow manipulation of bacteria...

...Antibody-independent methods may include analysis of transcriptomic or proteomic data from several different tissues... feasible and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want...tissues. You know that there is a wealth of transcriptomic data from this sample type, so you use your...

... few prior publications to go on, a submitted manuscript could only have been viewed as a significant ...require working knowledge of the command line or scripting. Input flexibility: Software tools require some...machine. On the offline front, there is a Python script called SSFinder, but I find its implementation ...

... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding...can even be used to detect a single copy of a transcript. But this sensitivity can sometimes cause as ...

...Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, they found that fusing dCas9 to...to an Mxi1 repressor domain repressed transcription of HSP90 an essential C. albicans gene. Read the ...

...ability to direct enzymes – from nucleases to transcription factors – to specific sequences of DNA has become...of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014...

...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...

...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...

... resource.  This is a labor-intensive project; scripts can be used in some cases, but much of this work...information about a cell line you’ve used? Submit your manuscript or data to Cellosaurus via [email protected]...

... that everyone can access data and drafts of manuscripts and grant applications in real-time so they can...figure out are timelines for experiments and manuscripts, who is responsible for which parts of the project...

...elements described below.   Vector Element Description Origin of Replication (ORI) DNA sequence which...for further study. Promoter Region Drives transcription of the target gene. Vital component for expression...