We narrowed to 372 results for: ena
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TypeBlog PostPublishedJuly 6, 2023, 1 p.m....simply – they are systems that measure biological phenomena by generating an output which is directly proportionate...directly measure a cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension...
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Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....the AAV retrograde serotype (AAVrg) and how it enables scientists to access neurons, and study their connections... across broader physical areas of the brain and enable access to neurons that are physically blocked, ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m.... biosensor - IISER Bhopal is back in the SynBio arena this year with a fresh team of twenty excited undergrads...expression under low temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....Bradner and Gray labs, we synthesize compounds that enable selective removal of proteins-of-interest from ...that are available from Addgene. These plasmids enable lentiviral expression of N- or C-terminally FKBP12F36V-tagged... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m.... Cas9 (FIRE–Cas9), combines these approaches to enable rapid and reversible recruitment of chromatin complexes...El-Samad lab predicts that this level of control will enable future researchers to better simulate physiological... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....molecule in the brain. “We hope that iGluSnFR3 will enable significant steps forward in our understanding ...may help us to create high-performance sensors to enable multiplexed imaging of neurotransmitter, calcium... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....properties when paired with PHP.eB’s systemic delivery enables minimally-invasive gene delivery and optogenetic...Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics. Nat Methods 16... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....stable gene expression? Constant gene expression can enable more confidence in gene expression readouts from...and Christopher A. Voigt. "Engineered promoters enable constant gene expression at any copy number in ... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m....Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial genetics." ...al. "Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....Other information may also be critical to the maintenance and use of certain plasmids, such as special ...for the inserts or the full plasmids. This will enable you to quickly BLAST your sequencing result against... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....integrate permanently into the host genome and thereby enable long-term stable gene expression. Gamma-retroviral.... This empty MuLV vector from the Weinberg lab enables researchers to express siRNAs of their choice in... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m.... identify new therapeutic targets/solutions and enable scientists to discover if known drugs can be used...rapid test for the functional activity of VUSs will enable more accurate diagnosis of NFIX-related syndromes... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....that blood flows through the heart and lungs and oxygenates the body. However, in neuroscience, this architecture... (e.g., optogenetic or chemogenetic receptors) enables complex control of specifically defined neuronal... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....with their recent plasmid deposit. These plasmids enable two main types of experiments: Whole mount in ...specific gene expression. The 49 deposited plasmids enable students to study gene expression and functions... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....transfect the cells, these large regions of homology enable us to effectively utilize the host cell’s inherent...tagging with fluorescent proteins as described here enables live cell imaging to study cellular structures ... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m..../10.1021/cn500325v Goutaudier, R., Coizet, V., Carcenac, C., & Carnicella, S. (2019). DREADDs: The Power...Olanzapine: A potent agonist at the hM4D(Gi) DREADD amenable to clinical Additional resources on the Addgene... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....toxic substances like heavy metals or pesticides, enabling their hosts to survive where others can’t. This...diversity of natural plasmids and their functions has enabled researchers to develop and deposit the many plasmid... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....what MoClo is and what types of experiments it enables. What is modular cloning? Modular cloning, or MoClo...gene, and terminator. Fig 1. MoClo enables individual parts to be assembled into larger functional... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....sites. Step aside, transcriptionally active dioxygenase When I started to work on building a CRISPR/... TET proteins are not demethylases; they are dioxygenases that oxidize 5-methylcytosine to create rare... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....Allen Brain Atlas. The comparison with two atlases enables us to see the reproducibility and accuracy while...from antibodies that we screen. This atlas will enable scientists to have an intuitive guide for checking...