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Showing: 101 - 120 of 212 results

  1. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...antibody is ideal as it will help to amplify the signal. Some applications use either direct (primary-conjugated..., remember that a secondary should be used when signal amplification is needed.  Species  Next, check ...

  3. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...Gutless Ad maintain only the ITRs and the packaging signal (Ψ), both of which are essential for assembly of...sequences and retain only the ITRs and packaging signal in cis. Therefore, co-infection with a helper adenovirus...

  6. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because of its...pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators...

  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...light to detect, measure, and control molecular signals, cells, and groups of cells in order to understand... into distinct populations based on fluorescent signal. Thus, FACS can be used to separate cells expressing...

  9. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat an ELISA plate...during incubation steps in order to enhance the OD signals and then complain about high background. In many...

  11. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...-expressing E. coli. The GV-specific ultrasound signal inside the tumors was overlaid with the anatomical... in situ expression. The GV-specific ultrasound signal is in hot and the anatomical B-mode images are ...

  12. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...including the nervous system, development, cell signaling, aging, and mechanotransduction to name a few....Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid that...

  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...binding sites found on avidin are also ideal for signal amplification during these experiments. During ...interest leading to amplification of the reporter signal and easier detection. This strategy is referred...

  14. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...SunTag scaffold, the intensity of the fluorescent signal is boosted and enables single molecule tracking... T cell receptor portion of the CAR facilitates signal transduction and release of cytotoxic granules ...

  16. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...particle (the packaging signal Psi). Because the packaging plasmids lacked the psi signal, the genes encoding...

  19. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...already been used for includes work probing mTOR signaling in cancer cells, deciphering the mechanism of ...
Showing: 101 - 120 of 212 results