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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 322 results for: nans

Showing: 101 - 120 of 322 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans DNA editing with STITCHR...11012), and counterstained with DAPI. New recombinant antibodies targeting Glypicans By Ashley Waldron...) recently released a set of highly-specific recombinant antibodies against three of the six human glypican...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... Central PMCID: PMC393301. Da Silva, Nancy A. & Sneha Srikrishnan. “Introduction and expression of genes...expression and largely defines the amount of recombinant protein that will be produced. Many commonly-...

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...components can be electroporated into the oviduct of pregnant females, a technique called Genome editing via...injection into either an oocyte or into the oviduct of pregnant females (Yoon et al., 2018, Mizuno et al., 2018...in vivo genome editing in mouse embryos using recombinant adeno-associated viruses. Nat Commun 9: . https...

  4. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...and should not be confined by birth country or financial means. Instead, talent should be realized through...globalized environment re-emerging from international financial struggle, our new generations feel thrown, to ...twitter @zcastan or on linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard...

  5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...largest source among those mentioned above. The contaminants can spread through dirty clothing, lab wears...should be made available for cell culturing and maintenance of the cell lines. Providing such an environment...for the lab to avoid bringing in environmental contaminants.  Figure 2: An Addgenie works in the...

  6. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...2004): 473. PubMed PMID: 15565146. 24. Nandakumar, Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "...protocols, including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor...

  7. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...-limiting step for efficient transduction by recombinant adeno-associated virus vectors." Journal of virology...10. Wu, Jianqing, et al. "Self-complementary recombinant adeno-associated viral vectors: packaging capacity...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...imaged with stimulated emission depletion (STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with ...& Bewersdorf, J. (2016). Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications... R. Y. (1998). Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells. Science,...

  9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...light (~400 nm), the chromophore photoconverts predominantly to the anionic form, causing increased fluorescence...multilabel imaging and dual color fluorescence nanoscopy." Nature biotechnology 26.9 (2008): 1035-1040....fluorescent proteins for far-field fluorescence nanoscopy." Biophysical journal 95.6 (2008): 2989-2997. ...

  10. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...EcoFlex a great option for those interested in recombinant protein purification and metabolic engineering...expression, the system includes a toolkit to express recombinant proteins within the mitochondrial matrix.   ...localization and transport studies in HEK cells, recombinant protein purification in bacterial cells, and ...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...expresses the λ Red recombinase enzymes, as well as a dominant-negative mutator allele of the E. coli MMR protein...and SnoopTag/SnoopCatcher The development of recombinant DNA technology has allowed molecular biologists...wine, fuels, pharmaceutical ingredients, and recombinant proteins. In order to keep production yields ...containing long homology arms and both auxotrophic and dominant selection markers, allowing for use with both ...

  12. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of integrin...sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access to IPI...

  13. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...manufacturer. You can also consider using monoclonal or recombinant antibodies, which have much lower lot-to-lot ...point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal...enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly...

  14. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We ...Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions...

  15. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...

  16. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...Croteau, J., Thakur, N., Jonigk, D., Davis, J. L., Hernandez, J. M., & Germain, R. N. (2022). IBEX: An iterative...Beuschel, R. T., Kabat, J., Croteau, J., Davis, J., Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile...

  17. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...reporters, such as Renilla luciferase (RLuc) or NanoLuc, require injection of substrate to produce bioluminescence...References 1 Bourdeau, R.W., Lee-Gosselin, A., Lakshmanan, A., Farhadi, A., Kumar, S.R., Nety, S.P., & ...

  19. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...

  20. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...One function that rose to the challenge was our Finance Team. With Addgene closed for almost two weeks ...for unknown amounts of time, we were facing a financial crisis. As a nonprofit organization, Addgene charges...
Showing: 101 - 120 of 322 results