...largest source among those mentioned above. The contaminants can spread through dirty clothing, lab wears...should be made available for cell culturing and maintenance of the cell lines. Providing such an environment...for the lab to avoid bringing in environmental contaminants.  Figure 2: An Addgenie works in the...

...imaged with stimulated emission depletion (STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with ...& Bewersdorf, J. (2016). Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications... R. Y. (1998). Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells. Science,...

...2004): 473. PubMed PMID: 15565146. 24. Nandakumar, Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "...protocols, including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor...

...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...-limiting step for efficient transduction by recombinant adeno-associated virus vectors." Journal of virology...10. Wu, Jianqing, et al. "Self-complementary recombinant adeno-associated viral vectors: packaging capacity...

...EcoFlex a great option for those interested in recombinant protein purification and metabolic engineering...expression, the system includes a toolkit to express recombinant proteins within the mitochondrial matrix.   ...localization and transport studies in HEK cells, recombinant protein purification in bacterial cells, and ...

...light (~400 nm), the chromophore photoconverts predominantly to the anionic form, causing increased fluorescence...multilabel imaging and dual color fluorescence nanoscopy." Nature biotechnology 26.9 (2008): 1035-1040....fluorescent proteins for far-field fluorescence nanoscopy." Biophysical journal 95.6 (2008): 2989-2997. ...

...expresses the λ Red recombinase enzymes, as well as a dominant-negative mutator allele of the E. coli MMR protein...and SnoopTag/SnoopCatcher The development of recombinant DNA technology has allowed molecular biologists...wine, fuels, pharmaceutical ingredients, and recombinant proteins. In order to keep production yields ...containing long homology arms and both auxotrophic and dominant selection markers, allowing for use with both ...

...collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of integrin...sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access to IPI...

...manufacturer. You can also consider using monoclonal or recombinant antibodies, which have much lower lot-to-lot ...point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal...enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly...

... their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We ...Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions...

...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...

...Croteau, J., Thakur, N., Jonigk, D., Davis, J. L., Hernandez, J. M., & Germain, R. N. (2022). IBEX: An iterative...Beuschel, R. T., Kabat, J., Croteau, J., Davis, J., Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile...

...reporters, such as Renilla luciferase (RLuc) or NanoLuc, require injection of substrate to produce bioluminescence...References 1 Bourdeau, R.W., Lee-Gosselin, A., Lakshmanan, A., Farhadi, A., Kumar, S.R., Nety, S.P., & ...

...LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins will then be screened for toxicity...oxidase. The goal is to determine potential water contaminants and to subsequently develop technologies to ...

... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...

...fragment, single-chain variable fragment (scFv), or a nanobody is one potential remedy for this problem within... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

...One function that rose to the challenge was our Finance Team. With Addgene closed for almost two weeks ...for unknown amounts of time, we were facing a financial crisis. As a nonprofit organization, Addgene charges...

...a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...

...how punctuation can be wildly funny as well as financially critical. Those who work on the publishing side...the right audiences and how open access affects financial models in information publication. Take a look...

...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...-5247(20)30004-5 McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M, Manzel L, Shi L, Netland J, Jia HP...