Skip to main content

We narrowed to 326 results for: nans

Showing: 101 - 120 of 326 results
  1. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ..., Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose or magnetic...their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was developed, which can...on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...
  2. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...implemented and from that, develop better software.  Maintenance  We’re not just about developing new features...: we take time during each sprint to do some maintenance work on our code. One way to look at this is ...continuing to add new features.  Another area of maintenance is agreeing to the names of the things in our...
  3. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...to the daily news briefing, to quick notes, to finances, to flights & ordering food - everything is covered...significant barrier to ELN adoption. This includes financial outlay, staff hours, troubleshooting, and the ... long-term use is likely to require on-going maintenance and support.” Besides that, academic institutions...
  4. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... Central PMCID: PMC393301. Da Silva, Nancy A. & Sneha Srikrishnan. “Introduction and expression of genes...expression and largely defines the amount of recombinant protein that will be produced. Many commonly-...
  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans DNA editing with STITCHR...11012), and counterstained with DAPI. New recombinant antibodies targeting Glypicans By Ashley Waldron...) recently released a set of highly-specific recombinant antibodies against three of the six human glypican...
  6. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...components can be electroporated into the oviduct of pregnant females, a technique called Genome editing via...injection into either an oocyte or into the oviduct of pregnant females (Yoon et al., 2018, Mizuno et al., 2018...in vivo genome editing in mouse embryos using recombinant adeno-associated viruses. Nat Commun 9: . https...
  7. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...and should not be confined by birth country or financial means. Instead, talent should be realized through...globalized environment re-emerging from international financial struggle, our new generations feel thrown, to ...twitter @zcastan or on linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard...
  8. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...2004): 473. PubMed PMID: 15565146. 24. Nandakumar, Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "...protocols, including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor...
  9. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...-limiting step for efficient transduction by recombinant adeno-associated virus vectors." Journal of virology...10. Wu, Jianqing, et al. "Self-complementary recombinant adeno-associated viral vectors: packaging capacity...
  10. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...largest source among those mentioned above. The contaminants can spread through dirty clothing, lab wears...should be made available for cell culturing and maintenance of the cell lines. Providing such an environment...for the lab to avoid bringing in environmental contaminants.  Figure 2: An Addgenie works in the...
  11. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...light (~400 nm), the chromophore photoconverts predominantly to the anionic form, causing increased fluorescence...multilabel imaging and dual color fluorescence nanoscopy." Nature biotechnology 26.9 (2008): 1035-1040....fluorescent proteins for far-field fluorescence nanoscopy." Biophysical journal 95.6 (2008): 2989-2997. ...
  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...EcoFlex a great option for those interested in recombinant protein purification and metabolic engineering...expression, the system includes a toolkit to express recombinant proteins within the mitochondrial matrix.   ...localization and transport studies in HEK cells, recombinant protein purification in bacterial cells, and ...
  13. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...imaged with stimulated emission depletion (STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with ...& Bewersdorf, J. (2016). Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications... R. Y. (1998). Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells. Science,...
  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...expresses the λ Red recombinase enzymes, as well as a dominant-negative mutator allele of the E. coli MMR protein...and SnoopTag/SnoopCatcher The development of recombinant DNA technology has allowed molecular biologists...wine, fuels, pharmaceutical ingredients, and recombinant proteins. In order to keep production yields ...containing long homology arms and both auxotrophic and dominant selection markers, allowing for use with both ...
  15. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of integrin...sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access to IPI...
  16. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...manufacturer. You can also consider using monoclonal or recombinant antibodies, which have much lower lot-to-lot ...point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal...enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly...
  17. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We ...Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions...
  18. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...
  19. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...Croteau, J., Thakur, N., Jonigk, D., Davis, J. L., Hernandez, J. M., & Germain, R. N. (2022). IBEX: An iterative...Beuschel, R. T., Kabat, J., Croteau, J., Davis, J., Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile...
  20. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...reporters, such as Renilla luciferase (RLuc) or NanoLuc, require injection of substrate to produce bioluminescence...References 1 Bourdeau, R.W., Lee-Gosselin, A., Lakshmanan, A., Farhadi, A., Kumar, S.R., Nety, S.P., & ...
Showing: 101 - 120 of 326 results