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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 230 results for: pET

Showing: 101 - 120 of 230 results

  1. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...your primers should be free of palindromic and repetitive sequences, but if present, a minor extension ...template should be isolated from a methylation competent bacterial strain (e.g. DH5α which is dam+). Methylation...

  2. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...equipment, categorize all of your minor tools, like pipets, or mini-centrifuges, etc. Be sure to capture as...phones.  As for reagents and consumables (such as pipet tips, glassware, etc), get a simple list from your...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005). Although this...Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 ...

  4. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation...

  6. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...hypotheses that they are involved in plasmid competition or can hijack machinery from other CRISPR systems...CRISPR–Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids Research, 48(...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Unnatural amino acid incorporation in mammalian cells Peter Schultz and colleagues at the Scripps Research Institute...Giannaks et al 2003, Bert et al 2000). In 2000, Peter Cockerill and his lab developed an improved luciferase...available, as well as 5S RNA F30-2xdBroccoli fusion and pET28c-F30-Broccoli controls. Don’t just eat more broccoli...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...et al. Cell Systems. 2016. PubMed PMID: 28189581 Peters J, et al. Cell. 2016. PubMed PMID: 27238023 Listen...

  11. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...master fundamental technical lab skills, like pipetting! We’ll release a new tip every two weeks during...

  12. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...submission.    Our Cultured Warrior (made by Nate using pipette boxes, wire, cable ties) received a festive makeover...

  14. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates – it’s time to turn ...

  15. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ... In the future, you’ll be able to access these snippets here or from their respective articles on the ...

  16. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...organization we thrive on 3 things: fun, teamwork, and competition. And our Halloween celebration this Friday will...

  20. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...accompanying Nature News & Views Andreas Möglich and Peter Hegemann described several advantages of the new...
Showing: 101 - 120 of 230 results