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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 226 results for: poly

Showing: 101 - 120 of 226 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...sample is first amplified using RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-...as well as health-related single nucleotide polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...inosine), which is treated as ‘G’ by cellular polymerases. Subsequently, an original genomic A-T base pair...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

  3. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... challenges of science becoming increasingly politicized. It would be nearly impossible for a career scientist...science in a “fake news and post-truth era,” the politicization of science (from climate change deniers to ...

  4. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

  5. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org ...

  7. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ... in Biology and Biotechnology from Worcester Polytechnic Institute. What is RNAi? The loss-of-function... in Biology and Biotechnology from Worcester Polytechnic Institute where he investigated how plants achieve...

  8. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ... you will find will recommend using a polycation, like polybrene (protocol) or DEAE dextran (protocol)...

  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...rXRCC1, or the DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion... proteins whose fusion improved the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...of assembling these multi-protein fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher...sequential additions have been made, the final polyproteam can be eluted using maltose. This procedure allows...

  16. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ... Gut to BrainResearchers: Atharva Sahasrabudhe; Polina Aniveeka   A big thank you to all who have contributed...

  18. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, ...
Showing: 101 - 120 of 226 results