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...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

...VSV-G is also toxic at high concentrations as described above and is inactivated by the complement system...proteins that can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al...

...If you’re new to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role...MECP2 in Rett Syndrome Rett Syndrome was first described in 1966 by Austrian physician, Andreas Rett, who...

...their BioRxiv post, the authors generated and described several improved genetically encoded fluorescent...Takayuki Iwaki, Kimiko Hara, and Kazuo Umemura have described a simple method which alleviates the inclusion...

...Cas13a as a diagnostic tool, as discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   ... the collateral RNA cleavage response is well described in bacterial cells, LwaCas13a does not display...

...positive control, the internal control vector, was described earlier. Once you are sure your conditions are...come from multiple, independent sources. We'll describe these a bit more below, but the papers cited in...

...addition of IPTG. T7 RNAP is then available to transcribe the gene of interest from a T7 promoter on the...don't see protein overexpression? The strains described above should generate sufficient expression levels...

...same fluorescent markers, Brainbow 3.1 and 3.2 (described in the same publication) are unique because by...cell type. The Confetti mouse was first used to describe the self-renewal of cells in the intestinal crypt...

..., and ultraviolet light. In this post, we will describe the mechanism of homology directed repair, one...(Quadros et al., 2017). Easi-CRISPR in vitro transcribes RNA encoding your repair template, then uses ...

...R26R-Confetti described in Hugo J. Snippert, et al. (2010) and the MAGIC Marker strategy described in Karine...

...Dr. Theodor Escherich, the scientist who first described them in 1885. E. coli are mainly found in the ...Expression Systems. Check out our companion post describing protein expression strains, where we go over ...

...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse ...'d use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene plasmids...

...confirmations like linear and nicked plasmids, as described in this article.   Long-read sequencing Addgene...bacterial colonies using one of the detection methods described above can help ensure the isolation of the monomeric...

...for and how can you do it well? Below, I briefly describe some tips for a successful postdoc fellowship.... there a tractable question or are you simply describing a phenomenon? Exploration can be important, but...

...brightness of individual fluorophores. Table 1 describes the brightness of a few commonly used fluorophores...panel! Designing a Panel In this example, I will describe a basic panel (Table 2, Figure 5) for a hypothetical...

...see: Uhlen et al., 2016). In this post, we will describe the approaches that can be used to determine selectivity...recognized by either antibody was not shared. Tables describe that the antibodies both recognized the target...

...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong et al. (2013) Science ...co-transfect in HR donor. Alternatively, a new system described recently is the double nickase system, with PX335...

...citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines recombineering...plasmid tools that can be used in this way. As described in the above CRISPRi section, the Marraffini Lab...

...different antibody isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful ...