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We narrowed to 286 results for: tac

Showing: 101 - 120 of 286 results

  1. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...are small plastic containers that can easily be attached with a hose to a nitrogen gas tank with a pressure...encoded by the plasmids in the library will remain attached to the membrane at the same location that their...

  2. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...unedited animals struck me as a “needle in the haystack” type of problem and led to me to explore alternate...lethal mutation should remove much of the unedited “haystack”, facilitating recovery of edited animals. ...

  3. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... to mechanism. Do the necessary tools exist to tackle the problem and if so, do you have ways to access... you gave a presentation to a specific group and tack on a few new pieces of data, your talk will probably...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...dsDNA for proper base pairing through minor grove contacts, and thus do not read specific base sequences,... ensure fidelity by interrogating minor groove contacts." Nucleic acids research 32.15 (2004): 4503-4511...

  5. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...What’s my angle on attacking this problem?”, try thinking “Who can I assemble to attack this problem from...

  6. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ... analysis, you'll need to use fluorophores that attach to the target. When you design a flow experiment...cells with fluorophore-conjugated antibodies that attach to a specific target. By using a mix of different...

  7. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest....9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ... of DNA bases and leave the core ring structure intact, allowing them to make 4 out of the possible 12...for our next post about base editing, where we’ll tackle transversion editors for the three other DNA bases...

  10. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...a double-membrane transfer with a psi membrane stacked behind the p membrane. If your protein runs through...., & Chen, Y. (2011). Prolonged Incubation and Stacked Film Exposure Improve Sensitivity in Western Blotting...

  11. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...Andrew, the head of our scientific support team, to tackle any experimental questions you may have. Our Director...

  13. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...beyond the basics - diving into the ins and outs, tackling complex questions, and giving tips for consistently...

  15. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...Looger New serotype pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik New viral vector ...

  16. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...fill out our Guest Blogger Form and we'll be in contact with you ASAP. Happy Reading! Tyler ...

  19. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...their boxes with shipping labels and paperwork. Our stacks of Addgene boxes are now ready to be sent around...
Showing: 101 - 120 of 286 results