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...RNA-seq clustering of cells transduced with the TF pooled library. Louvain community clusters of individual... system. TF screens can be designed to identify custom TF programs to acutely initiate disease onset, ...reference if you are looking to understand a pathway, or just looking for the best astrocyte differentiation protocol...s something you’d like that’s not yet available, just ask an Addgene scientist at [email protected]! References...

...dimensional, as you can see by this post’s chromatin illustrations from Leah Bury of Microscopic Art. I started... genes, and blog posts are not comprehensive, so just because you do not see a name or gene on this list...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...consider searching directly on our website. In-exhaustive Chromatin-related genes with plasmids on Addgene.org...articles in our collection; the following articles are just a small cross-section spanning 20 years of research...

...design a custom primer. Because Sanger sequencing can usually sequence just 1 kb of DNA, custom primers...the plasmid doesn’t contain the insert, you will just see the vector backbone band. Or, you can choose...see two bands but if the plasmid doesn’t, you will just see one band. These are only a few examples of how...

... blogger Kate Palozola Traditional lab notebooks just won't cut it for bioinformatics. All kinds of biologists...me, and I hope that it is helpful to those of you just getting started. The practices suggested here may...directory is a file system in the computer and is just another name for a folder. For instance, your Desktop...section about the edit that was temporarily used. Just be sure that the original code remains in tact! ... of code does are very useful for those that are just starting out, and helps clarify the role of the ...

...recently published paper, Yinong Yang’s group did just that as part of their CRISPR work in rice plants...molecules in a cell, this system is clearly also robust enough to ensure high levels of gRNA cleavage.   .... This highly efficient multiplexing further illustrates the potential of PTGs. Applications of PTGs The...advantage of the PTG system is its universality; robust and efficient tRNA processing is common to prokaryotes...examine their function. Bacteria and viruses have illustrated the power of polycistronic gene organization,...

...you can be incredibly frustrating. Clarifying the words —> decreasing frustration —> increasing the happiness...context yourself. Simple words are not so simple Just as our lab used jargon that required context to ...service,” did he mean it the same way as Bryan, our customer support manager? When Gizela, the aforementioned...for their field or technology in a consistent way. Just creating a lexicon was not enough. We also had to...

...post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo Colombi..., there are few general parameters that you can adjust: temperature and time of incubation, and amount... antibiotic has been used. Don’t worry too much, just pick the big colony and you are safe! Colony screening...University. Oskar Laur, PhD runs Emory University DNA Custom Cloning Service since 2009. He has  developed a...he has used for cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds...

...cognitive load you are asking your audience to take on, just to understand you at the most basic of levels.  ...summarize the concepts you’re covering. Don't show frustration if they're incorrect; take it instead as valuable...your audience and when things aren't working out, adjust the ask.  I used to do a lot of outreach to student...describing something verbally. Sometimes a group was just really tired and needed a slower, simpler presentation...information than normal. I had to learn to observe and adjust on the fly.  Once you can think about everything...

...was in the job description, but this skill is not just about reacting to technical issues. This skill also...Perhaps the Scientific Method? Imagine your problem is just a question you have about the world. You might do...training.  Developing strong problem solving skills is just a matter of applying that framework to the myriad...experiment you had to troubleshoot; you probably did not just automatically come to a solution. Consider the thoughts...strong problem solving skills alone can take you - just because I can resolve issues effectively does not...

...Public speaking is not just one of the most common phobias–it’s a key part of being a scientist and an...re-oriented, continued presenting. The whole process took just under a minute. I can't say the journal club was... You’re muted on Zoom again.  These things don’t just happen to you or me (though, let's be clear, they...the incidents above–and the dumpster noises were just last week!  Nerves It’s completely normal to be ...informative presentation, speak at conferences, or even just provide quick updates during a company meeting increases...

...transcriptional activation The project began with a frustration many researchers have experienced: wanting to...expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted to control...construct into the mouse retina. GFP expression robustly activated the reporter gene TdTomato, whose expression...TdTomato expressing cells were GFP+, indicating a robust, but specific system. T-DDOGs also successfully...seen with T-DDOGs. Like T-DDOGs, Cre-DOG is both robust and specific. When tested in retinal electroporation...

...sites such that custom TAL arrays could be constructed quickly and efficiently in just a few steps. More...part of the recognition sequence, they can be customized to direct assembly of DNA fragments. When designed...avoid undesired digestion, the Type IIS site used must not be present within the fragments you seek to ...Nov 5. PubMed. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...

... you to relevant primary research articles. Then just start reading and following citations through the...background in your field, but sometimes papers are also just poorly written. By nature, scientific literature... what it means. The introduction should give you just enough information to allow you to understand the...article for the first time. The first time through, just get an idea of the techniques used in the research...solve your literature organization issues – F1000 just announced the launch of their F1000Workspace.  Give...

...stories and plenty of people to vouch for them. Trust your gut – only work with someone you connect with...tagline is Bridging Science and Business. Maybe you just need help getting through your PhD? Some coaches... of transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist...We Turn Academics into Confident and Successful Industry Professionals”. This larger shop might appeal ...excellent in coaching for communication skills. Industry transition is also an area of specialty for Michelle...

...is a more emphasis today about non-academia and industry jobs. Since there are a variety of different career...Technician. “I was so tired of being in school so I just wanted to work,” she said. “School will always be...excited to see where my life will take me. I’m also just learning. I have a huge interest in data science... knows that although her focus is finance, it is just as important to bring love and fun to the office...much less fearful of making mistakes, because it is just part of the process. Additional resources on the...

...occur several constraints must be met. The donor and acceptor fluorophores must be compatible, such that...to sufficient spectral overlap, the fluorophores must be located within 1-10nm of each other and be oriented... FRET for imaging biomolecules in live cells (Sustarsic and Kapanidis 2015), which may lead to monitoring...18479457. PubMed Central PMCID: PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the...

...practice, and honing of your skills from the start. Just like with any sport, hours of practice are required...findings from our own or other groups, but your work must be original (otherwise why would you be interested...can provide different insight into an area - not just working within the established network of your peers...at first (and second) you won’t succeed, but you must try, try again. Now, this does not mean that you...should apply for everything that’s out there, it just means that you shouldn’t give up when your applications...

... 53 really is right there, at the end of helix 3…just like we said it was”.   Seeing is believing. And...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...conformational flexibility that the Cas9 protein must have in order to scan along the double-stranded ...endonuclease active sites. Fortunately, there is never just one model of a protein. We now have another model... the conformational gymnastics that this protein must undergo as it interrogates your genome. If you are...

...fact if a multicopy variant of a gene exists, there must be assays that researchers can use to quantify said...number assays for years. qPCR works like regular PCR, just with signaling molecules added to the mastermix ...assays, samples, standard curves, and normal controls must be run in triplicate.  The nice thing about qPCR...needed Do you need to call exact copy numbers or just general trends? How confident do you need to be ... Aziz, A. T., Shueb, R. H., Zaid, M., Sim, B., Mustafa, M., Nik Yusof, N. K., Lee, C. K., Abu Bakar, S...

...cause bad reactions in mice. Customization AAV offers a level of customization that is unmatched by other...over the past decade. CRISPR screens are easily customizable and enable the targeting of hundreds to tens...lentivirus but offer an improved safety profile, customization, and enhanced expression in many mouse tissues...combination of serotypes and psuedotypes means you can customize your CRISPR screen to a high degree. This is particularly...