We narrowed to 904 results for: CAN

...process of starting a forum where non-scientists can directly approach scientists and doctors with their...visual representation of science to our audience. How can researchers work with you? We welcome researchers...contributing to The Addictive Brain. A contribution can either be an article, a short post, a video, or a...

... we have to change to ensure that all scientists can have successful careers doing science in some form...the upcoming "Future of Research" conference. You can register for the conference here. The Future of Research...programs or peer mentoring groups. But for now, you can click here to register and join your postdoc colleagues...

...DNA sequencing troubleshooting and analysis 1. You can use any of the following programs to view your .ab1...all inclusive service some companies offer), you can purify the product either via the Sodium Acetate/...strongly recommend using a silica spin column. They can be pricey, but well worth it. 6. Edit your DNA ...

...tools.  Distributing and depositing In Figure 1, you can see cumulative numbers of CRISPR plasmid distribution...categorized by function, from 2014 to 2023. As you can see, nucleases, the original CRISPR tool, remain ...the most popular with our requestors. However, we can again see quick adaptation and steady use of all ...categorized by “age” or year they were deposited, you can see that from 2013 and on, popular CRISPR plasmids...ordering the plasmids is the easy part; using them can be a bit more challenging. Addgene’s free educational...other countries, from Japan to Australia, are significant depositors and requestors. CRISPR tools are developed...

...cell genome can be packaged inside the vectors during production. These impurities cannot be removed during...capabilities and Seqwell’s plexWell technology, we can now perform NGS at Addgene to sequence all the DNA.... In most cases, the source of the contamination can be identified, and we will discard any AAV prep we...developed custom open-access Python scripts that can perform a deeper analysis of the data. Our serotype...Next-generation sequencing (NGS) is a powerful tool that can be used to identify DNA contaminants in AAV preparations...

...were first designed in 2005. The DNA-binding domain can be engineered to target specific DNA sequences while...then induces a double strand break. These breaks can then be repaired by homologous recombination with...triplet and when several ZFNs are strung together, it can read many base pairs simultaneously to increase specificity...geneticist named Zobrist who designs a virus that can potentially kill one-third of the human beings (just...rely on the cellular DNA repair machinery, which can lead to high frequency of indel formation in the ...called pegRNA (prime editing RNA), the prime editor can install all possible point mutations, delete short... understanding of the adenine base editors which can help accelerate the engineering of novel base editor...

...variants are more permissive: SpG can target NGN PAMs, while SpRY can target both NAN and NGN PAMs effectively...base editing doesn’t necessarily mean researchers can fix any problematic C or A mutations in the genome...retained a small amount of RNA-editing activity, which can cause off-target effects in a variety of RNA transcripts...right PAM Expanding PAM preferences Base editors can only access a short sequence, between four and eight...preference for specific PAMs. Though near-PAMless Cas9s can broadly target many sites in the genome, they also...double-stranded breaks in the DNA backbone, making them significantly less prone to indel mutations that plagued CRISPR...scientist who uses Addgene to share their tools. We can’t wait to see what you create next!   References...

...critical control in biological experiments. Replicates can be technical or biological and are included to improve...such as bovine serum albumin. Secondary antibodies can also react with endogenous antibodies naturally present...in mouse tissue. Such species-on-species staining can be partially addressed with stringent staining protocols...way to reduce, if not eliminate, the noise. This can be done either by modifying the blocking conditions...autofluorescence, also interferes with data interpretation and can be addressed with a label control. In a label control...allows you to detect dead or dying cells so that you can base your analysis on living cells only (for example...results, the signal from your target needs to be significantly higher than the background noise (Waters 2009...

... to specific subcellular regions, laser ablation can damage other cellular structures, and genetic mutations...Restricting the effect to a specific time or location can be critical when, say, the gene is essential during...with dozens of family members and many functions it can be difficult to unravel their specific roles. Zebrafish...using directed evolution (Tei et al., 2023).  PLD can hydrolyze phosphatidylcholine (PC), the predominant... membrane lipid, to phosphatidic acid (PA) or it can swap phosphatidylchonine’s choline head group for...to plasma membrane or lysosomes and its activity can be monitored by phosphatidic acid probe GFP-PASS....

.... ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune...scFv that binds cancer-related antigens, binding of the scFv part of the receptor can activate T cells...are the smallest unit of an antibody molecule that can bind antigen. While they may seem unfamiliar, scFvs...both VL-linker-VH and VH-linker-VL configurations can generate functional scFvs; however, some individual...scFvs are easier and cheaper to make because they can be expressed in bacteria, while mAbs generally require... Power., 2019). Their rapid clearance from blood can be a drawback for therapeutic applications where ...cells to kill cancer cells. scFv’s small size also allows for their delivery by viral vectors like AAV, which...