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We narrowed to 892 results for: CAN

Showing: 591 - 600 of 892 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    .... ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune...scFv that binds cancer-related antigens, binding of the scFv part of the receptor can activate T cells...are the smallest unit of an antibody molecule that can bind antigen. While they may seem unfamiliar, scFvs...both VL-linker-VH and VH-linker-VL configurations can generate functional scFvs; however, some individual...scFvs are easier and cheaper to make because they can be expressed in bacteria, while mAbs generally require... Power., 2019). Their rapid clearance from blood can be a drawback for therapeutic applications where ...cells to kill cancer cells. scFv’s small size also allows for their delivery by viral vectors like AAV, which...

  2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that ...from infected cells. Read on to learn how CRISPR can take on HIV-1 in vitro, and what obstacles must be...healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life ...promoter-driven GFP expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused...our reach. Similar studies have shown that CRISPR can be used to combat other viral infections, notably...learned in pursuing one technological application can benefit many others and accelerate the pace of research...inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune response to destroy these...

  3. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...Without Cre recombination, RFP is expressed. Cre can mediate one of two deletions to allow YFP or M-CFP...using 2 loxP variants (only identical loxP sites can mediate recombination.) The deletions are mutually...deletion and inversion. The Brainbow-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-... two color possibilities. The remaining cassette can invert as long as Cre remains expressed; once Cre...includes a mutant YFP that does not fluoresce, but can be detected via immunostaining. This feature facilitates...placed under different promoters, Brainbow and Flpbow can be used to label distinct cell populations. To decrease...three recombinations, and subsequent recombinations cannot occur. While the Brainbow-1 system employs Cre-...

  4. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... approaches everything; we asked ourselves, “How can we do this in a way that most helps scientists and...about virus production protocols and quality control can be found on Addgene’s Viral Production webpage). ...partnerships to expand the service and figure out what we can afford to do and when. Addgene’s Legal Team acquired...Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in Your Research Using Virus...

  5. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ... promoters, has limited the number of gRNAs that can be packaged into viral vectors for potential in vivo...the Gao Lab demonstrated that the tRNA-gRNA array can drive the expression of itself, without using any...gene editing with DAP array   Use DAP arrays can enable efficient multiplex gene editing, such as ...different tRNAs with optimized 5’ leader sequences can be used together in DAP array. To construct DAP array...their initial testing. It outperformed other tRNA candidates (either from human or plant sources) in both ...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...a variety of roles in synaptic communication and can trigger other forms of neuronal signaling and regulation... The yellow (v82) and lime green (v857) versions can be summarized quickly as: iGluSnFR3 v82: increased...being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski Lab...provided a brief breakdown of the promoter systems you can use to express the iGluSnFR3 variants: pRSET: Bacterial...

  7. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...space per user, but, if you need more space, you can sign up for a plan. Best of all, by not using paper...also a very helpful aspect to this app; the user can search by sequence, category of enzyme, or name to..., and structures into one convenient resource. I can safely say that I still have the single and triple...codon list for each amino acid, but, overall AMINO can be beneficial for new researchers or students beginning...your notebook. The files uploaded are saved and cannot be deleted, so there is no worry about misplacing...find an MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet ...quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS book, this is a great resource...

  8. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...foods, and fibers. And because working with plants can be challenging, there are a lot of unexplored areas...not food, not medicine, and not mandatory – people can decide for themselves if they want this beautiful...body of knowledge. Organizations like Open Plant can then build on this research and effectively carry... pursuing scientific or business enterprises, he can be found climbing mountains or playing with his two...plant biology is a significant technical obstacle to plant synthetic biology. We cannot build what we do...now synonymous with GM to the point that people cannot imagine any use for GM technology other than to...sales. Flowers that change color throughout the day cannot be developed through conventional breeding; synthetic...

  9. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...manuscript needs to be revised before it can be published, this can delay publication and manuscripts may...that the manuscript requires revisions, the authors can publish a new version of the article, while the first...effort) and the scientific community, as the reports can be very informative as to how a paper is received...journals and their scientific communities aim to significantly improve the reproducibility and reliability ...

  10. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    Published
    March 16, 2016, 5:30 p.m.
    ...suggestions. Can’t find a plasmid you’re looking for in the repository? Let us know! You can notify us to...plasmid and a publication where we can find the plasmid. While we cannot guarantee that everyone we reach...
Showing: 591 - 600 of 892 results